Influence of Fe³⁺ on the activity of Na,K-ATPase.

<p>(A) Activity of Na,K-ATPase in erythrocyte membranes of individuals with or without iron overload: n = 24 (case) and n = 12 (control), p<0.0001 (Mann Whitney). (B) Activity of Na,K-ATPase in erythrocyte membranes of subjects with or without iron overload, stratified by age group: n = 12 (<50 years), n = 06 (control <50 years), n = 12 (> = 50 years), n = 06 (Control > = 50 years). p = 0.002 (case x control <50 years), p = 0.005 (case x control > = 50 years), Student's t test. All experiments were performed in triplicates and the line in bars represented the mean and the error bar denotes SEM.</p

Main clinical manifestations reported by patients with hyperferritinemia grouped by age.

<p>* X²</p><p>^<b>†</b> n = 10</p><p>^<b>‡</b> n = 11</p><p>Main clinical manifestations reported by patients with hyperferritinemia grouped by age.</p

Iron overload effect on oxidative stress.

<p>(A) Content of Fe³⁺ in the residual plasma of individuals with or without iron overload: n = 24 (case) and n = 14 (control), p = 0.087 (Mann Whitney). (B) Lipid peroxidation in subjects with or without iron overload: n = 14 (case) and n = 14 (control), p<0.0001 (Student's t test). All experiments were performed in triplicate and the line in bars represented the mean and the error bar denotes SEM.</p

Influence of Fe³⁺ on the activity of Na,K-ATPase.

<p>(A) Activity of Na,K-ATPase in erythrocyte membranes of individuals with or without iron overload: n = 24 (case) and n = 12 (control), p<0.0001 (Mann Whitney). (B) Activity of Na,K-ATPase in erythrocyte membranes of subjects with or without iron overload, stratified by age group: n = 12 (<50 years), n = 06 (control <50 years), n = 12 (> = 50 years), n = 06 (Control > = 50 years). p = 0.002 (case x control <50 years), p = 0.005 (case x control > = 50 years), Student's t test. All experiments were performed in triplicates and the line in bars represented the mean and the error bar denotes SEM.</p

Influence of iron overload on lipid profile of the erythrocyte plasma membrane.

<p>(A) Total phospholipids: n = 23 (case) and n = 14 (control), p = 0.013 (Student's t test). (B) Total phospholipids of patients with iron overload, stratified by age: n = 11 (<50 years) and n = 12 (> = 50 years), p = 0.554 (Student's t test). (C) Total membrane cholesterol: n = 23 (case) and n = 14 (control), p<0.001 (Student's t test). (D) Total membrane cholesterol of patients with iron overload, stratified by age: n = 11 (<50 years) and n = 12 (> = 50 years), p = 0.241 (Student's t test). All experiments were performed in triplicate and the line in bars represented the mean and the error bar denotes SEM.</p

H₂O₂ and iron effect on red blood cell.

<p>Red blood cell from health donators were incubated with 0.8 mM H₂O₂ or 1 μM FeCl₃ for 24h at 4°C. This sample was used to obtained: (A) plasma TBARS levels, (B) the Na,K-ATPase activity of membrane preparation and the total levels of (C) cholesterol and (D) phospholipids. All experiments were performed in triplicates and the line in bars represented the mean and the error bar denotes SEM, *p <0.05; results are significantly different from controls, as evaluated by ANOVA test.</p