9 research outputs found
Characteristics of 151 Brazilian Sporothrix schenckii Isolates from 5 Different Geographic Regions of Brazil: A Forgotten and Re-Emergent Pathogen
Abstract: We used RAPD (random amplification of polymorphic DNA) to analyze the phenotypic and genotypic characteristics of Sporothrix schenckii isolated from five geographic regions of Brazil, from clinical, animal, and environmental sources. Our results yielded a significant difference (P<0.01) in the mean conidial area of S. schenckii animal isolates (2.96 ± 1.07) compared with those of clinical isolates (fixed form, 2.33 ± 0.53; lymphocutaneous form, 2.37 ± 0.43). There was no association among S. schenckii clinical isolates and geographic region. Isolates from the Northeast region exhibited the lowest thermotolerance (% growth inhibition) at 35ÂșC ( x = 49.23% ± 17.25) and at 37ÂșC (70.43% ± 10.93%). Northern isolates exhibited the highest thermotolerance at 35ÂșC (12.82% ± 5.73%) and at 37ÂșC (23.81% ± 8.27%). RAPD with a 10-mer primer OPD-18 generated 67 PCR fingerprint patterns. The 151 S. schenckii isolates fell into seven major clusters with such great genetic diversity that an association of isolates with clinical forms or geographic areas could not be determined, even with investigations focused on more restricted geographic areas. The main physiological characteristics of Brazilian S. schenckii isolates were also characterized, including osmophilia, halophilia, pH tolerance, urease activity, casein hydrolysis, and gelatinase, proteinase, and DNAase production
Effect adjuvant of synthetic oligonucleotides(CpG-ODN) from P. brasiliensis gp43 gene in the Th2-Th1 immunomodulation of experimental murine paracoccidiodomycosis
BV UNIFESP: Teses e dissertaçÔe
AnĂĄlise ProteĂŽmica Comparativa De Isolados De P. Brasiliensis E Sua Relação Com A VirulĂȘncia FĂșngica
Paracoccidioidomycosis (Pcm) Is A Systemic Mycosis Commonly Found In Latin America That Is Caused By Two Distinct Species Of Paracoccidioides Genus, Paracoccidioides Brasiliensis Complex (S1, Ps2, Ps3 E Ps4) And Paracoccidioides Lutzii. Its Pathobiology Has Been Recently Explored By Different Approaches To Clarify The Mechanisms Of Host-Pathogen Interactions Underpinning Pcm. The Diversity Of Clinical Forms Of This Disease Has Been Attributed To Both Host- And Fungus-Related Factors. For Better Understanding Of The Molecular Underpinnings Of Host-Fungus Interactions, We Evaluated In Vivo Virulence Of Nine Paracoccidoides Brasiliensis Isolates And Correlated It To Protein Expression Profiles Obtained By Two-Dimensional Gel Electrophoresis. Based On The Recovery Of Viable Fungi From Mouse Organs, The Isolates Were Classified As Those Having Low, Moderate, Or High Virulence. Highly Virulent Isolates Overexpressed Proteins Related To Adhesion Process And Stress Response, Probably Indicating Important Roles Of ThoA Paracoccidioidomicose (Pcm) Ă Uma Micose SistĂȘmica EndĂȘmica Na AmĂ©rica Latina, Causada Por EspĂ©cies Do GĂȘnero Paracoccidioides, Paracoccidioides Brasiliensis E Paracoccidioides Lutzii. A Diversidade De Formas ClĂnicas Da Pcm Tem Sido AtribuĂda Tanto A Fatores Relacionados Ă Resposta Imune Do Hospedeiro Quanto A Fatores IntrĂnsecos Ao Fungo. Desta Forma, Para Desenvolver Ferramentas DiagnĂłsticas E TerapĂȘuticas Ă Importante Elucidar Como O Fungo Interage Com O Hospedeiro E Ainda Se A ExpressĂŁo De Determinadas ProteĂnas Se Relaciona Com A VirulĂȘncia FĂșngica. Assim, NĂłs Avaliamos O Perfil De VirulĂȘncia De Nove Isolados De P. Brasiliensis E Relacionamos A VirulĂȘncia FĂșngica A ExpressĂŁo Diferencial De ProteĂnas Por Meio Da AnĂĄlise ProteĂŽmica. Os Isolados De P. Brasiliensis Foram Classificados Em Diferentes Graus De VirulĂȘncia, Alto, MĂ©dio E Baixo, Com Base Na Contagem De Fungos ViĂĄveis Nos ĂrgĂŁos De Camundongos Balb/ E B10. Isolados Apresentando Alta VirulĂȘncia Induziram A ExpressĂŁo De ProteĂnas Relacionadas Ă AdDados abertos - Sucupira - Teses e dissertaçÔes (2018
Proteomic analysis of Paracoccidioides brasiliensis complex isolates: Correlation of the levels of differentially expressed proteins with in vivo virulence.
BACKGROUND:Paracoccidioidomycosis (PCM) is a systemic mycosis commonly found in Latin America that is caused by distinct species of Paracoccidioides genus: Paracoccidioides brasiliensis complex (S1, PS2, PS3 and PS4) and Paracoccidioides lutzii. Its pathobiology has been recently explored by different approaches to clarify the mechanisms of host-pathogen interactions underpinning PCM. The diversity of clinical forms of this disease has been attributed to both host- and fungus-related factors. METHODOLOGY/PRINCIPAL FINDINGS:For better understanding of the molecular underpinnings of host-fungus interactions, we evaluated in vivo virulence of nine Paracoccidioides brasiliensis complex isolates and correlated it to protein expression profiles obtained by two-dimensional gel electrophoresis. Based on the recovery of viable fungi from mouse organs, the isolates were classified as those having low, moderate, or high virulence. Highly virulent isolates overexpressed proteins related to adhesion process and stress response, probably indicating important roles of those fungal proteins in regulating the colonization capacity, survival, and ability to escape host immune system reaction. Moreover, highly virulent isolates exhibited enhanced expression of glycolytic pathway enzymes concomitantly with repressed expression of succinyl-CoA ligase beta chain, a protein related to the tricarboxylic acid cycle. CONCLUSIONS/SIGNIFICANCE:Our findings may point to the mechanisms used by highly virulent P. brasiliensis isolates to withstand host immune reactions and to adapt to transient iron availability as strategies to survive and overcome stress conditions inside the host