7 research outputs found
Venezuelan Equine Encephalitis Virus in Iquitos, Peru: Urban Transmission of a Sylvatic Strain
Enzootic strains of Venezuelan equine encephalitis virus (VEEV) have been isolated from febrile patients in the Peruvian Amazon Basin at low but consistent levels since the early 1990s. Through a clinic-based febrile surveillance program, we detected an outbreak of VEEV infections in Iquitos, Peru, in the first half of 2006. The majority of these patients resided within urban areas of Iquitos, with no report of recent travel outside the city. To characterize the risk factors for VEEV infection within the city, an antibody prevalence study was carried out in a geographically stratified sample of urban areas of Iquitos. Additionally, entomological surveys were conducted to determine if previously incriminated vectors of enzootic VEEV were present within the city. We found that greater than 23% of Iquitos residents carried neutralizing antibodies against VEEV, with significant associations between increased antibody prevalence and age, occupation, mosquito net use, and overnight travel. Furthermore, potential vector mosquitoes were widely distributed across the city. Our results suggest that while VEEV infection is more common in rural areas, transmission also occurs within urban areas of Iquitos, and that further studies are warranted to identify the precise vectors and reservoirs involved in urban VEEV transmission
Zika virus infection in Nicaraguan households
<div><p>Zika virus (ZIKV) infection recently caused major epidemics in the Americas and is linked to congenital birth defects and Guillain-Barré Syndrome. A pilot study of ZIKV infection in Nicaraguan households was conducted from August 31 to October 21, 2016, in Managua, Nicaragua. We enrolled 33 laboratory-confirmed Zika index cases and their household members (109 contacts) and followed them on days 3–4, 6–7, 9–10, and 21, collecting serum/plasma, urine, and saliva specimens along with clinical, demographic, and socio-economic status information. Collected samples were processed by rRT-PCR to determine viral load (VL) and duration of detectable ZIKV RNA in human bodily fluids. At enrollment, 11 (10%) contacts were ZIKV rRT-PCR-positive and 23 (21%) were positive by IgM antibodies; 3 incident cases were detected during the study period. Twenty of 33 (61%) index households had contacts with ZIKV infection, with an average of 1.9 (range 1–6) positive contacts per household, and in 60% of these households, ≥50% of the members were positive for ZIKV infection. Analysis of clinical information allowed us to estimate the symptomatic to asymptomatic (S:A) ratio of 14:23 (1:1.6) among the contacts, finding 62% of the infections to be asymptomatic. The maximum number of days during which ZIKV RNA was detected was 7 days post-symptom onset in saliva and serum/plasma and 22 days in urine. Overall, VL levels in serum/plasma, saliva, and urine specimens were comparable, with means of 5.6, 5.3 and 4.5 log<sub>10</sub> copies/ml respectively, with serum attaining the highest VL peak at 8.1 log<sub>10</sub> copies/ml. Detecting ZIKV RNA in saliva over a similar time-period and level as in serum/plasma indicates that saliva could potentially serve as a more accessible diagnostic sample. Finding the majority of infections to be asymptomatic emphasizes the importance of silent ZIKV transmission and helps inform public health interventions in the region and globally.</p></div
Risk factor analysis for ZIKV infection among contacts (n = 109).
<p>Risk factor analysis for ZIKV infection among contacts (n = 109).</p
ZIKV viral load kinetics over time in bodily fluid samples (serum/plasma, saliva and urine) by day post-symptom onset, collected in enrolled households in Managua, August to October, 2016.
<p>Mean and standard deviation of viral load in bodily fluids plotted according to day post-symptom onset.</p
Demographic characteristics, ZIKV infection and symptomatic Zika status among study participants in Managua, Nicaragua, August to October, 2016.
<p>Demographic characteristics, ZIKV infection and symptomatic Zika status among study participants in Managua, Nicaragua, August to October, 2016.</p
Flow chart of study design and enrollment for index cluster study of ZIKV infection in Managua, Nicaragua, 2016.
<p>*One enrolled contact did not answer the individual questionnaire but fulfilled the other study requirements such as symptom questionnaire and sample donation.</p
ZIKV infection positivity and percent symptomatic contacts among enrolled households in Managua, Nicaragua, August to October, 2016.
<p>ZIKV infection positivity and percent symptomatic contacts among enrolled households in Managua, Nicaragua, August to October, 2016.</p