Mamoeiro: amostragem para diagnose foliar.

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Fertirrigação em fruteiras tropicais.

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Lixiviação de nutrientes em uma área cultivada com meloeiro irrigado.

O presente trabalho teve como objetivo avaliar o efeito da fertirrigação na cultura do meloeiro, irrigado de forma localizada em um solo arenoso, sobre a lixiviação de nutrientes no perfil do solo. As profundidades de amostragem: solo foram: 0 a 20; 20 a 40 e 40 a 60 cm. As amostras foram coletadas em quatro períodos durante o desenvolvimento da cultura, sempre antes da fertirrigação e 1, 2 e 3 dias após a fertirrigação. Determinou-se pH, P, K, Ca, Mg, Na, acidez potencial e soma de bases, capacidade de troca de cations e saturação por bases. Os resultados mostraram aumentos nos teores de P, Ca e Mg nas camadas abaixo de 20 cm. Os teores de pH, K, H+Al, e V pouco variaram ao longo das camadas estudadas. Tendo sido observados efeitos da fertirrigação no aumento dos teores de K. Os resultados mostram uma tendência de melhoria da fertilidade nas camadas abaixo dos 20 cm em função do uso da fertirrigação

Chronic hemolytic anemia is associated with a new glucose-6-phosphate dehydrogenase in-frame deletion in an older woman

Glucose-6-phosphate dehydrogenase (G6PD) deficiency, an X-linked disorder, is usually observed in hemizygote males and very rarely in females. The G6PD class 1 variants, very uncommon, are associated with chronic hemolytic anemia. Here we report a Portuguese woman who suffered in her sixties from a chronic hemolytic anemia due to G6PD deficiency. Molecular studies revealed heterozygosity for an in-frame 18-bp deletion, mapping to exon 10 leading to a deletion of 6 residues, 362-367 (LNERKA), which is a novel G6PD class 1 variant, G6PD Tondela. Two of her three daughters, asymptomatic, with G6PD activity within the normal range, are heterozygous for the same deletion. The patient's leukocyte and reticulocyte mRNA studies revealed an almost exclusive expression of the mutant allele, explaining the chronic hemolytic anemia. Patient whole blood genomic DNA HUMARA assay showed a balanced pattern of X chromosome inactivation (XCI), but granulocyte DNA showed extensive skewing, harboring the mutated allele, implying that in whole blood, lymphocyte DNA, with a very long lifetime, may cover up the current high XCI skewing. This observation indicates that HUMARA assay in women should be assessed in granulocytes and not in total leukocytes

Synergistic Antifungal Study of PEGylated Graphene Oxides and Copper Nanoparticles against Candida albicans

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).The coupling reactions of polyethylene glycol (PEG) with two different nano-carbonaceous materials, graphene oxide (GO) and expanded graphene oxide (EGO), were achieved by amide bond formations. These reactions yielded PEGylated graphene oxides, GO-PEG and EGO-PEG. Whilst presence of the newly formed amide links (NH-CO) were confirmed by FTIR stretches observed at 1732 cm−1 and 1712 cm−1, the associated Raman D- and G-bands resonated at 1311/1318 cm−1 and 1584/1595 cm−1 had shown the carbonaceous structures in both PEGylated products remain unchanged. Whilst SEM images revealed the nano-sheet structures in all the GO derivatives (GO/EGO and GO-PEG/EGO-PEG), TEM images clearly showed the nano-structures of both GO-PEG and EGO-PEG had undergone significant morphological changes from their starting materials after the PEGylated processes. The successful PEGylations were also indicated by the change of pH values measured in the starting GO/EGO (pH 2.6–3.3) and the PEGylated GO-PEG/EGO-PEG (pH 6.6–6.9) products. Initial antifungal activities of selective metallic nanomaterials (ZnO and Cu) and the four GO derivatives were screened against Candida albicans using the in vitro cut-well method. Whilst the haemocytometer count indicated GO-PEG and copper nanoparticles (CuNPs) exhibited the best antifungal effects, the corresponding SEM images showed C. albicans had, respectively, undergone extensive shrinkage and porosity deformations. Synergistic antifungal effects all GO derivatives in various ratio of CuNPs combinations were determined by assessing C. albicans viabilities using broth dilution assays. The best synergistic effects were observed when a 30:70 ratio of GO/GO-PEG combined with CuNPs, where MIC50 185–225 μm/mL were recorded. Moreover, the decreased antifungal activities observed in EGO and EGO-PEG may be explained by their poor colloidal stability with increasing nanoparticle concentrations.Peer reviewe

A switch from high-fat to normal diet does not restore sperm quality but prevents metabolic syndrome

In recent decades, the prevalence of metabolic diseases has concomitantly increased with a decline on fertility rates and sperm quality. High-fat diets (HFD) are seldom considered part of the problem, but the molecular mechanisms underlying its effects on male fertility remain poorly understood. Herein we postulated that HFD alter sperm quality. We evaluated the effects of switching from a HFD to a normal diet in early adulthood on metabolic disease onset, testicular metabolism and sperm quality. Thirty-six male C57BL6/J mice were divided in: a control group fed with standard chow; a group fed with HFD for 200 days; and a group fed with HFD for 60 days and then with standard chow (HFDt). Biometric data and whole-body metabolism were assessed. Epididymal sperm was studied for concentration, motility, viability and morphology. 1H-NMR metabolomics approach was performed on testicular extracts to trace the metabolic changes. Diet switch reduced body weight and fat mass, preventing metabolic syndrome onset. However, sperm viability, motility and morphology were deteriorated by HFD consumption and not restored by diet switch. HFD induced irreversible changes in pyruvate and glutamate metabolism, ethanol degradation and ammonia recycling in testis. Furthermore, HFDt changed purine and cysteine metabolism, urea cycle, and glutathione content. Overall, HFD caused irreversible changes in testicular metabolism even after switching to normal diet. HFD feeding until early adulthood decreases sperm quality, which cannot be restored by diet switch or weight loss, even when development of metabolic syndrome is avoided

Uso de efluentes da carcinicultura de águas interiores na irrigação de arroz e melão.

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Custo comparativo de três fontes de matéria orgânica e uma poliacrilamida utilizadas como condicionadores de solo na produção do meloeiro.

Custo comparativo de três fontes de matéria orgânica e uma poliacrilamida utilizadas como condicionadores de solo na produção do meloeiro.bitstream/CNPAT-2010/5297/1/Ct-015.pd

Consequências do plantio de sementes oriundas de plantas enxertadas ("clones") ou de plantas de "pé-franco" de cajueiro.

Heterogeneidade da planta, castanha e amêndoa. Redução do potencial produtivo, dificuldades de manejo e ineficiência da produção. O que o produtor pode fazer para solucionar tais problemas.bitstream/CNPAT-2010/11945/1/Cj-006.pd

Avaliação do efeito da água de irrigação na germinação de sementes de cajueiro, gravioleira e aceroleira.

O objetivo deste trabalho é avaliar o desenvolvimento do cajueiro, da gravioleira e da aceroleira em condicões de irrigacão com vários tipos de água com teores diferentes de sais.bitstream/CNPAT-2010/11936/1/Pa-041.pd