2 research outputs found
Las buenas prácticas metodológicas en gestión de proyectos aplicadas a una compañía de seguridad informática
From an exploratory approach, it is intended to inform the reader from the concepts of project management, the problems presented in a company in terms of project management, where the adaptation of a methodological guide that articulates a tool for Measurement of the maturity of the PMI adapted to an IT Security SME, which aligns the strategic business objectives and the organizational culture according to their needs.Desde un acercamiento exploratorio, se pretende dar a conocer al lector a partir de los conceptos de gestión de proyectos, la problemática presentada en una empresa en cuanto a la administración de proyectos, donde se plantea la adaptación de una guía metodológica que articule una herramienta de medición de la madurez del PMI adaptada a una Pyme de Seguridad Informática, que alinee los objetivos estratégicos empresariales y la cultura organizacional de acuerdo con sus necesidades
Role of heterotrimeric G protein and calcium in cardiomyocyte hypertrophy induced by IGF-1
In the heart, insulin‐like growth factor‐1 (IGF‐1) is a peptide with pro‐hypertrophic and anti‐apoptotic actions. The pro‐hypertrophic properties
of IGF‐1 have been attributed to the extracellular regulated kinase (ERK) pathway. Recently, we reported that IGF‐1 also increases intracellular
Ca2þ levels through a pertussis toxin (PTX)‐sensitive G protein. Here we investigate whether this Ca2þ signal is involved in IGF‐1‐induced
cardiomyocyte hypertrophy. Our results show that the IGF‐1‐induced increase in Ca2þ level is abolished by the IGF‐1 receptor tyrosine kinase
inhibitor AG538, PTX and the peptide inhibitor of Gbg signaling, bARKct. Increases in the activities of Ca2þ‐dependent enzymes calcineurin,
calmodulin kinase II (CaMKII), and protein kinase Ca (PKCa) were observed at 5 min after IGF‐1 exposure. AG538, PTX, bARKct, and the
dominant negative PKCa prevented the IGF‐1‐dependent phosphorylation of ERK1/2. Participation of calcineurin and CaMKII in ERK
phosphorylation was discounted. IGF‐1‐induced cardiomyocyte hypertrophy, determined by cell size and b‐myosin heavy chain (b‐MHC), was
prevented by AG538, PTX, bARKct, dominant negative PKCa, and the MEK1/2 inhibitor PD98059. Inhibition of calcineurin with CAIN did not
abolish IGF‐1‐induced cardiac hypertrophy. We conclude that IGF‐1 induces hypertrophy in cultured cardiomyocytes by activation of the
receptor tyrosine kinase activity/bg‐subunits of a PTX‐sensitive G protein/Ca2þ/PKCa/ERK pathway without the participation of calcineurin