16 research outputs found
TOTAL POLYPHENOLS, FLAVONOIDS CONTENTS AND ANTIOXIDANT ACTIVITY OF ROSA SP. GENOTIPES FROM DIFFERENT ALTITUDE OF ROMANIAN REGIONS
Eight wild rose hip genotypes from different altitudes varying from 3m to 902m were analyzed in order to evaluate the total polyphenols, flavonoids content and the antioxidant activity. The Rosa spp.rosehips collected from the Northeastern and the Southeasternof Romanianwere, as follows: Rosa canina, R. caesia, R. corymbifera, R. micrantha, R. nitidula, R. rubiginosa, R. subcanina, and R. vosagiaca. In some genotypes, the level of flavonoids and polyphenol content increased with the increasing altitude while in other it was observed a decrease. Polyphenol content reached a maximum of 144.36 mg GAE/g DW in R. rubiginosa whereas the lowest content of 61.72 mg GAE/g DW was recorded in R. caesia. The highest polyphenol content was reached at altitude of 860 m. The amount of flavonoids content ranged between 7.32 mg CE/g DW and 19.45 CE/g DW in R. caesia and in R. nitidula, respectively. The radical scavenging capacities of Rosa genotypes extracts were not positively correlated with altitude, except the R. corymbifera extractswhere the antioxidant activity increased with the increase of altitude
TEXTILE DYE BIOREMEDIATION POTENTIAL OF SOME RHIZOBIAL STRAINS AND THEIR HEAVY-METAL AND HIGH SALINITY TOLERANCE
The discharge of untreated textile dye effluents enriched with toxic pollutants including dyes, heavy metals and other hazardous materials may cause negative impacts on the entire ecosystem. The proposed work aimed to isolate, molecularly identify and characterize the native rhizobial strains with textile dye biodegradation potential in relation with their tolerance to high salinity and heavy metals (usually meet in high concentrations in the textile dye effluents). Native rhizobial strains were isolated from various terrestrial ecosystems originated in Danube – Delta Biosphere Reserve. Most of the strains tolerated ≥ 2.0% NaCl. Our data showed that 3 strains (Agrobacterium sp.CR-B19; Rhizobium giardinii CR- B22 and Ensifer sp.CR-B26) were able to tolerate 15 ppm concentration of cadmium (Cd2+), whereas all strains identified as Rhizobium sp. (except R. leguminosarum CR-B10), and Agrobacterium sp. could tolerate 70 ppm of chromium (Cr6+).. Moreover, 3 indigenous strains (Rhizobium giardinii CR-B13; Rhizobium sp.CR-B15 and Agrobacterium sp. CR-B19) tolerated a concentration of 200 ppm of lead (Pb2+). In regard to azo-dye degrading potential, only Rhizobium leguminosarum CR-B10 was able to degrade the Reactive Orange 16 dye (90.18% decolorization) in stationary conditions, at 30°C. Comparatively, Agrobacterium sp. CR - B19 strain removed Reactive Orange 16 (sulphonic azo-dye) (78.92 % decolorization) and Reactive Blue 4 (antraquinonic dye) (12 % decolorization) by adsorbtion. Based on their bioremediation potential, the newly isolated rhizobial strains could be further used (in pure culture or in consortia) to develop a new environmental friendly and cost–effective biotechnology in order to reduce the toxicity of textile dyes effluents
TEXTILE DYE BIOREMEDIATION POTENTIAL OF SOME RHIZOBIAL STRAINS AND THEIR HEAVY-METAL AND HIGH SALINITY TOLERANCE
The discharge of untreated textile dye effluents enriched with toxic pollutants including dyes, heavy metals and other hazardous materials may cause negative impacts on the entire ecosystem. The proposed work aimed to isolate, molecularly identify and characterize the native rhizobial strains with textile dye biodegradation potential in relation with their tolerance to high salinity and heavy metals (usually meet in high concentrations in the textile dye effluents). Native rhizobial strains were isolated from various terrestrial ecosystems originated in Danube – Delta Biosphere Reserve. Most of the strains tolerated ≥ 2.0% NaCl. Our data showed that 3 strains (Agrobacterium sp.CR-B19; Rhizobium giardinii CR- B22 and Ensifer sp.CR-B26) were able to tolerate 15 ppm concentration of cadmium (Cd2+), whereas all strains identified as Rhizobium sp. (except R. leguminosarum CR-B10), and Agrobacterium sp. could tolerate 70 ppm of chromium (Cr6+).. Moreover, 3 indigenous strains (Rhizobium giardinii CR-B13; Rhizobium sp.CR-B15 and Agrobacterium sp. CR-B19) tolerated a concentration of 200 ppm of lead (Pb2+). In regard to azo-dye degrading potential, only Rhizobium leguminosarum CR-B10 was able to degrade the Reactive Orange 16 dye (90.18% decolorization) in stationary conditions, at 30°C. Comparatively, Agrobacterium sp. CR - B19 strain removed Reactive Orange 16 (sulphonic azo-dye) (78.92 % decolorization) and Reactive Blue 4 (antraquinonic dye) (12 % decolorization) by adsorbtion. Based on their bioremediation potential, the newly isolated rhizobial strains could be further used (in pure culture or in consortia) to develop a new environmental friendly and cost–effective biotechnology in order to reduce the toxicity of textile dyes effluents
EVALUATION OF THE AMPLITUDE OF CYTOGENETIC DAMAGE INDUCED BY TWO FOOD ADDITIVES IN WHEAT
Sulfites and metabisulfites, nitrates and nitrites are among the most utilized food additives. They can determine
negative reactions in human body, but some of them showed genotoxic and carcinogenic action in various test-systems. In
this study, we analyzed the amplitude of the cytogenetic changes induced by the exposure to different concentrations
(0.1%, 0.5%, and 1.0%) of sodium nitrate (E251; SN) and sodium metabisulfite (E223; SMB) in wheat root tips
meristems, by scoring the mitotic index and the frequency of division phases, the rates of ana-telophase chromosome
aberrations, and of metaphase disturbances. Also, the influence on plantlet length was analyzed in young plantlets. The
two chemicals increased the amount of chromosome aberrations, depending on concentration and time exposure
– Changes induced by two chromium-containing compounds in antioxidative response, soluble protein level and amylase activity in barley seedlings
Chromium-induced changes in superoxide dismutase, catalase, peroxidase, amylase activity and in solubleprotein level have been analyzed in seedlings of Madalin barley cultivar after short exposure to 10, 100, 250, and 500 μM K2Cr2O7 and CrCl3.6H2O. Patterns of SOD and CAT are generally similar; in 5-days old seedlings the average values aresmaller than control, but in 9-days old seedlings the mechanisms of antioxidative defence are activated and the twoscavenging enzymes show values higher than control (up to 66 % in 500μM CrCl3). POD activity was inhibited - except10 μM concentration - independently of plantlet age. The level of soluble protein was lower than control at first determination, while in 9-days old plantlets the protein synthesis was inhibited (excepting 10 and 100 M K2Cr2O7, and 250 M CrCl3). Amylase was generally higher than control in 5d old seedlings, but decreased in the older ones</p
Evaluation of roundup-induced toxicity on genetic material and on length growth of barley seedlings
The study was performed in order to evaluate Roundup-induced genotoxic effects in Hordeum vulgare L.
cv. Madalin root meristems and to analyze herbicide impact on length growth of barley seedlings.
Caryopses were treated for 3 hours and 6 hours with 0.1%, 0.5%, 1.0% and 2.0% Roundup solutions
(v/v), containing 0.36 mg ml–1, 1.8 mg ml–1, 3.6 mg ml–1 and 7.2 mg ml–1 glyphosate active ingredient.
Mitotic index decreased in both exposure times with concentration increase. In 3-h treatment, its average
values decreased from 4.73±0.31% to 1.51±0.43%, whereas in 6-h treatment this parameter declined
from 3.86±0.92% to 0.62±0.15%. The highest ana-telophase aberration rates were noted in 3-h treatments
(8.91%, 9.19%, 9.47%, 11.25%, comparatively to control – 5.99%). Roundup enhanced the number
of metaphase disturbances proving its noxious effect on normal functioning of mitotic spindle. Seedling
growth was negatively influenced at all tested concentrations in both exposure times. The length
decreased as concentration increased, so that the average length is 7.5–9 times smaller than in control at
the maximum concentration, in both exposures
Reproducibility and dose dependency of the antitumoral pharmacodynamic effect of some autochthonous polysaccharidic or polyphenolic biopreparations of fungal and vegetal origin
The testing of the yeast Candida inconspicua in the decolorization process of waste coloured water, resulted
from the dye process of the textile fiber, revealed the presence of two possible mechanisms by azoic - dyes are removing
from effluents, as follow: adsorbtion, correlated with a low azoreductase activity, in the case of Acid Blue 113 and
biodegradation, as a result of reductive activity of azoreductase towards diazo = bonds, in the case of Basic Blue 41. The
experiment revealed also, the dependence of enzymatic activity by temperature