6,842 research outputs found
Resolving environmental drivers of microbial community structure in Antarctic soils
Antarctic soils are extremely cold, dry, and oligotrophic, yet harbour surprisingly high bacterial diversity. The severity of environmental conditions has constrained the development of multi-trophic communities, and species richness and distribution is thought to be driven primarily by abiotic factors. Sites in northern and southern Victoria Land were sampled for bacterial community structure and soil physicochemical properties in conjunction with the US and New Zealand Latitudinal Gradient Project. Bacterial community structure was determined using a high-resolution molecular fingerprinting method for 80 soil samples from Taylor Valley and Cape Hallett sites which are separated by five degrees of latitude and have distinct soil chemistry. Taylor Valley is part of the McMurdo Dry Valleys, while Cape Hallett is the site of a penguin rookery and contains ornithogenic soils. The influence of soil moisture, pH, conductivity, ammonia, nitrate, total nitrogen and organic carbon on community structure was revealed using Spearman rank correlation, Mantel test, and principal components analysis. High spatial variability was detected in bacterial communities and community structure was correlated with soil moisture and pH. Both unique and shared bacterial community members were detected at Taylor Valley and Cape Hallett despite the considerable distance between the sites
Impaired tumor growth and angiogenesis in mice heterozygous for Vegfr2 (Flk1)
Abstract VEGF signaling through its tyrosine kinase receptor, VEGFR2 (FLK1), is critical for tumor angiogenesis. Previous studies have identified a critical gene dosage effect of VegfA in embryonic development and vessel homeostasis, neovascularization, and tumor growth, and potent inhibitors of VEGFR2 have been used to treat a variety of cancers. Inhibition of FGFR signaling has also been considered as an antiangiogenic approach to treat a variety of cancers. Inhibition of VEGFR2 with neutralizing antibodies or with pharmacological inhibitors of the VEGFR tyrosine kinase domain has at least short-term efficacy with some cancers; however, also affects vessel homeostasis, leading to adverse complications. We investigate gene dosage effects of Vegfr2, Fgfr1, and Fgfr2 in three independent mouse models of tumorigenesis: two-stage skin chemical carcinogenesis, and sub-cutaneous transplantation of B16F0 melanoma and Lewis Lung Carcinoma (LLC). Mice heterozygous for Vegfr2 display profound defects in supporting tumor growth and angiogenesis. Unexpectedly, additional deletion of endothelial Fgfr1 and Fgfr2 in Vegfr2 heterozygous mice shows similar tumor growth and angiogenesis as the Vegfr2 heterozygous mice. Notably, hematopoietic deletion of two alleles of Vegfr2 had minimal impact on tumor growth, with little effect on angiogenesis, reinforcing the importance of endothelial Vegfr2 heterozygosity. These studies reveal previously unrecognized Vegfr2 gene dosage effects in tumor angiogenesis and a lack of synergy between VEGFR2 and endothelial FGFR1/2 signaling during tumor growth
An Extraordinary Scattered Broad Emission Line in a Type 2 QSO
An infrared-selected, narrow-line QSO has been found to exhibit an
extraordinarily broad Halpha emission line in polarized light. Both the extreme
width (35,000 km/sec full-width at zero intensity) and 3,000 km/sec redshift of
the line centroid with respect to the systemic velocity suggest emission in a
deep gravitational potential. An extremely red polarized continuum and partial
scattering of the narrow lines at a position angle common to the broad-line
emission imply extensive obscuration, with few unimpeded lines of sight to the
nucleus.Comment: 4 pages, 1 figure, to appear in the Astrophysical Journal Letter
Barcoding of the cytochrome oxidase I (COI) indicates a recent introduction of Ciona savignyi into New Zealand and provides a rapid method for Ciona species discrimination
Mitochondrial cytochrome oxidase I (COI) gene sequencing (DNA barcoding) of Ciona specimens from New Zealand (NZ) led to the first record of the solitary ascidian Ciona savignyi in the Southern Hemisphere. We sought to quantify C. savignyi COI genetic diversity around the NZ archipelago and to compare this with diversity within C. savignyi's native range in the north-west Pacific. Ciona savignyi specimens were collected from two NZ sites and from three sites around Japan. COI sequences (595 bp) were amplified and measures of genetic diversity were calculated. Based on differences between their COI sequences we developed a PCR-based assay to distinguish C. savignyi from the morphologically similar C. intestinalis. A total of 12 C. savignyi COI haplotypes were recovered from the 76 samples. Of the four haplotypes observed in NZ, two were unique. From the 10 haplotypes observed in the Japan samples, eight were unique. The C. savignyi populations in Japan were found to contain higher haplotype diversity when compared with those in NZ. The NZ samples contained only a small subset of the haplotype variation of the Japan samples, however, NZ samples did harbor two haplotypes not observed in the Japan samples. A PCR-based assay developed from the COI sequences was able to reliably discriminate the two Ciona species. The low COI genetic diversity within the two NZ C. savignyi populations sampled is consistent with a founder effect associated loss of genetic diversity. The robust PCR-based assay for distinguishing C. savignyi and C. intestinalis may find application in ecological and taxonomic studies and can be applied to both archival materials and live animals
Importance of Biologically Active Aurora-like Ultraviolet Emission: Stochastic Irradiation of Earth and Mars by Flares and Explosions
(Abridged) We show that sizeable fractions of incident ionizing radiation
from stochastic astrophysical sources can be redistributed to biologically and
chemically important UV wavelengths, a significant fraction of which can reach
the surface. This redistribution is mediated by secondary electrons, resulting
from Compton scattering and X-ray photoabsorption, with energies low enough to
excite atmospheric molecules and atoms, resulting in a rich aurora-like
spectrum. We calculate the fraction of energy redistributed into biologically
and chemically important wavelength regions for spectra characteristic of
stellar flares and supernovae using a Monte-Carlo transport code written for
this problem and then estimate the fraction of this energy that is transmitted
from the atmospheric altitudes of redistribution to the surface for a few
illustrative cases. Redistributed fractions are found to be of order 1%, even
in the presence of an ozone shield. This result implies that planetary
organisms will be subject to mutationally significant, if intermittent,
fluences of UV-B and harder radiation even in the presence of a narrow-band UV
shield like ozone. We also calculate the surficial transmitted fraction of
ionizing radiation and redistributed ultraviolet radiation for two illustrative
evolving Mars atmospheres whose initial surface pressures were 1 bar. Our
results suggest that coding organisms on planets orbiting low-mass stars (and
on the early Earth) may evolve very differently than on contemporary Earth,
with diversity and evolutionary rate controlled by a stochastically varying
mutation rate and frequent hypermutation episodes.Comment: 21 pages, 2 figures, accepted for publication in Origins of Life and
Evolution of the Biospher
Transport of Ionizing Radiation in Terrestrial-like Exoplanet Atmospheres
(Abridged) The propagation of ionizing radiation through model atmospheres of
terrestrial-like exoplanets is studied for a large range of column densities
and incident photon energies using a Monte Carlo code we have developed to
treat Compton scattering and photoabsorption. Incident spectra from parent star
flares, supernovae, and gamma-ray bursts are modeled and compared to energetic
particles in importance. We find that terrestrial-like exoplanets with
atmospheres thinner than about 100 g cm^-2 transmit and reprocess a significant
fraction of incident gamma-rays, producing a characteristic, flat surficial
spectrum. Thick atmospheres (>~ 100 g cm^-2) efficiently block even gamma-rays,
but nearly all incident energy is redistributed into diffuse UV and visible
aurora-like emission, increasing the effective atmospheric transmission by many
orders of magnitude. Depending on the presence of molecular UV absorbers and
atmospheric thickness, up to 10% of the incident energy can reach the surface
as UV reemission. For the Earth, between 2 x 10^-3 and 4 x 10^-2 of the
incident flux reaches the ground in the biologically effective 200--320 nm
range, depending on O_2/O_3 shielding. Finally, we suggest that transient
atmospheric ionization layers can be frequently created at low altitudes. We
conclude that these events can produce frequent fluctuations in atmospheric
ionization levels and surficial UV fluxes on terrestrial-like planets.Comment: 59 pages, 15 figures; in press in Icarus; minor edits, no results
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Addressing the mental health needs of the rural underserved: Findings from a multiple case study of a behavioral telehealth project
Behavioral telehealth is a reasonable solution to the accessibility to mental health care problem that exists in many rural communities. This paper reports the results of a multiple case study of a behavioral telehealth program administered through a marriage and family therapy training program. The results suggest that mental health services can be effectively delivered using existing distance education technology to underserved rural populations. Rural communities have unique barriers to accessing mental health care, some of which can be overcome through the distance delivery of services and some of which cannot. In order to effectively deliver treatment, accommodations to the technology must be made by both therapist and client
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