Dendrogram of intact alleles of <i>inlA</i>, and associated information.

<p>Alleles labeled with “1” and letters indicate sequences that were found in more than one isolate. Alleles labeled with “RM” and a number are unique alleles. Also listed are the Lineage information and MLVA types in which the alleles are found, watershed information, sample dates, and the number of isolates of each allele. Watershed information: A, Alisal; C, Carr Lake, G, Gabilan; S, Salinas River; T, Tembladero; X1 –X3, sites not associated with watersheds.</p

This cartoon summarizes the Penner types association into complexes commonly found in literature.

<p>The variable CPS biosynthesis loci of type strains belonging to the same complex present a high degree of homology [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144349#pone.0144349.ref031" target="_blank">31</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144349#pone.0144349.ref032" target="_blank">32</a>].</p

Illustration of PCR amplicons expected when using the updated <i>C</i>. <i>jejuni</i> CPS multiplex PCR.

<p>Lane 1, 100-bp NEB DNA standard; lane 2, mixture of PCR products obtained with all the templates from the alpha mix; lane 3, 100-bp NEB DNA standard; lane 4, mixture of PCR products obtained with all the templates from the beta mix; lane 5, 100-bp NEB DNA standard; lane 6, mixture of PCR products with all the templates from the gamma mix; lane 7, 100-bp NEB DNA standard; lane 8, mixture of PCR products with all the templates from the gamma mix.</p

Capsule multiplex PCR results among all <i>C</i>. <i>jejuni</i> isolates from different population group from South and South-East Asia.

<p>In bold are the five most prevalent CPS types in their respective group. Number in parentheses represent the number of strain in the category.</p

Bacterial strains used for analysis and validation of the capsule multiplex PCR.

<p>Bacterial strains used for analysis and validation of the capsule multiplex PCR.</p

Summary of the primer sequences included in the <i>C</i>. <i>jejuni</i> capsule multiplex typing scheme.

<p>^<i>a</i> Genbank accession number of the DNA sequence used to design the primers</p><p>^<i>b</i> Parkhill et al., 2000 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144349#pone.0144349.ref010" target="_blank">10</a>]</p><p>^<i>c</i> Poly et al., 2011 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144349#pone.0144349.ref032" target="_blank">32</a>]</p><p>^<i>d</i> HS4A represent HS4, HS13, HS16, HS43, HS50, HS62, HS64 and HS65</p><p>^<i>e</i> Pearson et al., 2007 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144349#pone.0144349.ref019" target="_blank">19</a>]</p><p>^<i>f</i> Karlyshev et al., 2005 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144349#pone.0144349.ref031" target="_blank">31</a>]</p><p>^<i>g</i> Fouts et al., 2005 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144349#pone.0144349.ref015" target="_blank">15</a>]</p><p>^<i>h</i> this study</p><p>^<i>i</i> HS4B includes CG8486, HS16 & HS64</p><p>^<i>j</i> Poly et al., 2007 [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144349#pone.0144349.ref014" target="_blank">14</a>]</p><p>Summary of the primer sequences included in the <i>C</i>. <i>jejuni</i> capsule multiplex typing scheme.</p