Three-dimensional pantograph for use in hazardous environments

Material measurement device is used with radioactive probes which can be approached only to distance of 3 feet. Tracer-following unit is capable of precisely controlled movement in X-Y-Z planes. Pantograph is usable in industrial processes involving chemical corrosives, poisons, and bacteriological hazards, as well as nuclear applications

Electronic flaw simulator for eddy current probe calibration

Electronic flaw simulator cycled into the eddy current system eliminates errors in probe calibration. A discrimination level reference established in the probe permits recognition of those flaws in materials with an equivalent volume equal to or greater than the reference

Synthesis of proteins by automated flow chemistry

Ribosomes can produce proteins in minutes and are largely constrained to proteinogenic amino acids. Here, we report highly efficient chemistry matched with an automated fast-flow instrument for the direct manufacturing of peptide chains up to 164 amino acids long over 327 consecutive reactions. The machine is rapid: Peptide chain elongation is complete in hours. We demonstrate the utility of this approach by the chemical synthesis of nine different protein chains that represent enzymes, structural units, and regulatory factors. After purification and folding, the synthetic materials display biophysical and enzymatic properties comparable to the biologically expressed proteins. High-fidelity automated flow chemistry is an alternative for producing single-domain proteins without the ribosome.National Science Foundation (Grant 1122374