Influence of metabolism and microbiology on organic micropollutants biotransformation in anoxic heterotrophic reactors

There is scarce information about the biotransformation of organic micropollutants (OMPs) under anoxic conditions. In this study, a heterotrophic denitrifying bioreactor was set up to study the fate of several OMPs from metabolic and microbiological points of view. Primary metabolic activity was increased by adding progressively higher nitrogen loading rates during the operation (from 0.075 to 0.4 g N-NO3- L−1 d−1), which resulted in an important shift in the microbial population from a specialized biomass to a more diverse community. Such a change provoked a significant increase in the removal efficiency of erythromycin (ERY), roxithromycin (ROX) and bisphenol-A (BPA), and some bacterial taxa, such as Rhodoplanes, were identified as possible indicators related to the biodegradation of these compounds. The increasing primary metabolic activity in the reactor did not enhance the OMP-specific removal rates, suggesting that the bacterial composition is more influential than cometabolismThis research was supported by the Spanish Research State Agency (AEI) through ANTARES (PID2019–110346RB-C21) project. M. Martinez-Quintela would also like to express his gratitude to the same agency for awarding a research scholarship (BES-2017–080503). All authors belong to the Galician Competitive Research Groups (GRC)_ ED431C-2021/37S