Comparative population genetics of the German shepherd dog in South Africa

Modern breeding practices strive to achieve distinctive phenotypic uniformity in breeds of dogs, but these strategies are associated with the inevitable loss of genetic diversity. Thus, in parallel with the morphological variation displayed by breeds, purebred dogs commonly express genetic defects as a result of the inbreeding associated with artificial selection and the reduction of selection against disease phenotypes. Microsatellite marker analyses of 15 polymorphic canine loci were used to investigate measures of genetic diversity and population differentiation within and between German-bred and South African-bred German shepherd dogs. These data were quantified by comparison with typically outbred mongrel or crossbred dogs. Both the imported and locally-bred German shepherd dogs exhibited similar levels of genetic diversity. The breed is characterised by only a moderate loss of genetic diversity relative to outbred dogs, despite originating from a single founding sire and experiencing extensive levels of inbreeding throughout the history of the breed. Non-significant population differentiation between the ancestral German and derived South African populations indicates sufficient contemporary gene flow between these populations, suggesting that migration resulting from the importation of breeding stock has mitigated the effects of random genetic drift and a population bottleneck caused by the original founder event in South Africa. Significant differentiation between the combined German shepherd dog population and the outbred dogs illustrates the effects of selection and genetic drift on the breed since its establishment just over 100 years ago

Fabrication and performance analysis of 4-sq cm indium tin oxide/InP photovoltaic solar cells

Large-area photovoltaic solar cells based on direct current magnetron sputter deposition of indium tin oxide (ITO) into single-crystal p-InP substrates demonstrated both the radiation hardness and high performance necessary for extraterrestrial applications. A small-scale production project was initiated in which approximately 50 ITO/InP cells are being produced. The procedures used in this small-scale production of 4-sq cm ITO/InP cells are presented and discussed. The discussion includes analyses of performance range of all available production cells, and device performance data of the best cells thus far produced. Additionally, processing experience gained from the production of these cells is discussed, indicating other issues that may be encountered when large-scale productions are begun

Using session-RPE to monitor training load in swimmers

The ability to measure and control the internal training load (TL) of athletes is important to optimize athletic performance. However, at present, there are no methods available for evaluating internal tl during swimming. The session-rpe method is a practical, non-invasive system used to quantify the internal tl placed on athletes. This article discusses how the session-rpe method may be used to monitor swim training and ultimately improve the training process of swimmers. Copyright © National Strength and Conditioning Association

Effects of ramadan intermittent fasting on sports performance and training: A review

The month-long diurnal Ramadan fast imposes a major challenge to Islamic athletes. Sporting events are programmed throughout the year, with the result that training and competition are often scheduled during Ramadan. The small numbers of well-controlled studies that have examined the effects of Ramadan on athletic performance suggest that few aspects of physical fitness are negatively affected, and only modest decrements are observed. Whereas subjective feelings of fatigue and other mood indicators are often cited as implying additional stress on the athlete throughout Ramadan, most studies show these measures may not be reflected in decreases in performance. The development and early implementation of sensible eating and sleeping strategies can greatly alleviate the disruptions to training and competitiveness, thus allowing the athlete to perform at a high level while undertaking the religious intermittent fast. Nevertheless, further research is required to understand the mechanisms and energy pathways that allow athletes to maintain their performance capacities during Ramadan, and which factors are responsible for the observed decrements in performance of some individuals. © Human Kinetics, Inc

Rugby sevens match demands and measurement of performance: A review

© 2018, University of Zagreb - Faculty of Kinesiology. All rights reserved. The purpose of this review is to summarize the research that has examined the match demands of elite-level, men’s rugby sevens, and provide enhanced understanding of the elements contributing to successful physical and technical performance. Forty-one studies were sourced from the electronic database of PubMed, Google Scholar and SPORTDiscus. From these, twelve original investigations were included in this review. Positive match outcomes are the result of an interplay of successful physical, technical, and tactical performances. The physical performance of players (activity profile measurement from GPS) includes high relative total distance and high-speed distance values in comparison to other team sports. The technical performance of players (skill involvement measurement from match statistics) involves the execution of a range of specific offensive and defensive skills to score points or prevent the opponent from scoring. The factors influencing change in these performance constructs has not been investigated in rugby sevens. There is a paucity in the literature surrounding the situational and individual factors affecting physical and skill performance in elite rugby sevens competition. Future studies should investigate the factors likely to have the strongest influence on player performance in rugby sevens. This should include larger sample sizes and account for repeated measures within players. This will allow coaches and scientists to improve their interpretation of activity and skill profile data, and make more informed decisions on players’ athletic preparation program

The Intrinsic Alignment of Dark Halo Substructures

We investigate the intrinsic alignments of dark halo substructures with their host halo major-axis orientations both analytically and numerically. Analytically, we derive the probability density distribution of the angles between the minor axes of the substructures and the major axes of their host halos from the physical principles, under the assumption that the substructure alignment on galaxy scale is a consequence of the tidal fields of the host halo gravitational potential. Numerically, we use a sample of four cluster-scale halos and their galaxy-scale substructures from recent high-resolution N-body simulations to measure the probability density distribution. We compare the numerical distribution with the analytic prediction, and find that the two results agree with each other very well. We conclude that our analytic model provides a quantitative physical explanation for the intrinsic alignment of dark halo substructures. We also discuss the possibility of discriminating our model from the anisotropic infall scenario by testing it against very large N-body simulations in the future.Comment: accepted version, ApJL in press, minor revision, 12 pages, 2 figure

Heat-acclimatization and pre-cooling: a further boost for endurance performance?

© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd To determine if pre-cooling (PC) following heat-acclimatization (HA) can further improve self-paced endurance performance in the heat, 13 male triathletes performed two 20-km cycling time-trials (TT) at 35 °C, 50% relative humidity, before and after an 8-day training camp, each time with (PC) or without (control) ice vest PC. Pacing strategies, physiological and perceptual responses were assessed during each TT. PC and HA induced moderate (+10 ± 18 W; effect size [ES] 4.4 ± 4.6%) and very large (+28 ± 19 W; ES 11.7 ± 4.1%) increases in power output (PO), respectively. The overall PC effect became unclear after HA (+4 ± 14 W; ES 1.4 ± 3.0%). However, pacing analysis revealed that PC remained transiently beneficial post-HA, i.e., during the first half of the TT. Both HA and PC pre-HA were characterized by an enhanced PO without increased cardio-thermoregulatory or perceptual disturbances, while post-HA PC only improved thermal comfort. PC improved 20-km TT performance in unacclimatized athletes, but an 8-day HA period attenuated the magnitude of this effect. The respective converging physiological responses to HA and PC may explain the blunting of PC effectiveness. However, perceptual benefits from PC can still account for the small alterations to pacing noted post-HA

Heart rate variability and direct current measurement characteristics in professional mixed martial arts athletes

This study’s purpose was to examine heart rate variability (HRV) and direct current potential (DC) measures’ sensitivity and correlations between changes in the acute recovery and stress scale (ARSS) and the previous day’s training load. Training load, HRV, DC and ARSS data were collected from fourteen professional mixed martial arts athletes (32.6 ± 5.3 years, 174.8 ± 8.8 cm, 79.2 ± 17.5 kg) the following morning after hard, easy and rest days. Sensitivity was expressed as a signal-to-noise ratio (SNR, inter-day typical error (TE) or coefficient of variation (%CV) divided by intra-day TE or %CV). Correlations between HRV, DC and ARSS with training load were also examined. The SNRs for the various HRV and DC measures were acceptable to good (1.02–2.85). There was a 23.1% CV average increase between measures taken between different locations versus the same location. Training load changes were not correlated with HRV/DC but were correlated with ARSS stress variables. Practitioners should be aware of HRV/DC variability; however the daily training signal was greater than the test-retest error in this investigation. Upon awakening, HRV/DC measures appear superior for standardization and planning. HRV and DC measures were less sensitive to the previous day’s training load than ARSS measures

Identification of genetic changes associated with drug resistance by reverse in situ hybridization.

The molecular cytogenetic techniques of comparative genomic hybridization (CGH) and reverse in situ hybridization (REVISH) allow the entire genomes of tumours to be screened for genetic changes without the requirement for specific probes or markers. In order to define the ability of REVISH to detect and map regions of amplification associated with drug resistance, we investigated a panel of cell lines selected for resistance to doxorubicin and intrinsic sensitivity to topoisomerase II-inhibitory drugs. We have defined a modified REVISH protocol, which involves double hybridizations with genomic DNA from the test cell lines and chromosome-specific whole chromosome paints to identify the chromosomes to which the amplicons localize. Sites of amplification are then mapped by fractional length measurements (Flpter), using published genome databases. Our findings show that amplification of the topoisomerase II alpha gene is readily detected and mapped, as is amplification of the MDR and MRP loci. Interestingly, REVISH detected a new amplicon in the doxorubicin-resistant lung cancer cell line, GLC4-ADR, which mapped to chromosome 1q. REVISH is therefore ideally suited to characterize genetic changes specific for drug resistance within a background of genetic anomalies associated with tumour progression