11 research outputs found
In-vitro evaluation of the PALL Leukotrap Affinity Prion Reduction Filter as a secondary device following primary leucoreduction
Improved leucoreduction of red blood cell units prepared after a 24-h hold with the platelet-rich plasma method using newly developed filters
Recombinant human immunoglobulin (Ig)A1 and IgA2 anti-D used for detection of IgA deficiency and anti-IgA
Prolonged post-thaw shelf life of red cells frozen without prefreeze removal of excess glycerol
Improved flow cytometric method to enumerate residual cells: Minimal linear detection limits for platelets, erythrocytes, and leukocytes
Thrombin generation and coagulation factor content of thawed plasma and platelet concentrates
Complement activation products in liquid stored plasma and C3a kinetics after transfusion of autologous plasma.
Background and Objectives Keeping a small stock of liquid plasma readily available for transfusion is common practise in Sweden. We report data on complement activation markers in plasma components during storage in the liquid state and the kinetics of C3a-(desArg) after transfusion of autologous plasma with high content of C3a-(desArg) . Material and Methods Plasma components were prepared by apheresis or from whole blood. C3 fragments (C3a-(desArg) , C3d,g, iC3), and soluble terminal complement complex (sC5b-9) were investigated. C3a-(desArg) kinetics was investigated in regular apheresis donors. Results Apheresis plasma prepared by membrane centrifugation had significantly higher level of C3a-(desArg) , C3d,g and sC5b-9 from day 0 and low iC3, than plasma prepared by other methods. By storage day 7, C3a-(desArg) -levels were above the reference value in 88% of all components. After re-infusion of autologous plasma with high C3a-(desArg) content, there were rapid a(1) and a(2) -distribution followed by a slower b-elimination phase. Conclusion Plasma components prepared by different methods and stored in the liquid phase differ significantly in the amount and timing of complement activation. C3a-(desArg) present in plasma is rapidly eliminated after transfusion. Autologous plasma could be used to study complement kinetics in different clinical situations