Immunological properties of gene vaccines delivered by different routes

Gene vaccines represent a new and promising approach to control infectious diseases, inducing a protective immune response in the appropriate host. Several routes and methods of genetic immunization have been shown to induce antibody production as well as T helper (Th) cell and cytotoxic T lymphocyte activation. However, few studies have compared the nature of the immune responses generated by different gene vaccination delivery systems. In the present study we reviewed some aspects of immunity induced by gene immunization and compared the immune responses produced by intramuscular (im) DNA injection to gene gun-mediated DNA transfer into the skin of BALB/c mice. Using a reporter gene coding for ß-galactosidase, we have demonstrated that im injection raised a predominantly Th1 response with mostly IgG2a anti-ßgal produced, while gene gun immunization induced a mixed Th1/Th2 profile with a balanced production of IgG2a and IgG1 subclasses. Distinct types of immune responses were generated by different methods of gene delivery. These findings have important implications for genetic vaccine design. Firstly, a combination between these two systems may create optimal conditions for the induction of a broad-based immune response. Alternatively, a particular gene vaccine delivery method might be used according to the immune response required for host protection. Here, we describe the characteristics of the immune response induced by gene vaccination and the properties of DNA involved in this process

Tendências no desenvolvimento de gases de proteção utilizados na soldagem MIG/MAG Trends in the development of protective gases used in GMAW welding

Este trabalho tem como objetivo apresentar uma revisão do estado da arte sobre os gases de proteção usados nos processos de soldagem a arco elétrico, com ênfase na soldagem MIG/MAG do aço. São apresentados os principais conceitos da física do arco e as propriedades relacionadas aos gases de proteção. O modelo de atmosfera ligeiramente oxidante para os gases de proteção é discutido, sendo mostradas as formulações dos gases estudados em artigos científicos e em patentes depositadas, bem como as reivindicações (vantagens) requeridas. Também são mencionados alguns poucos estudos usando adições de gases não convencionais. Foi observado que no desenvolvimento de gases de proteção para soldagem MIG/MAG do aço predomina o modelo da formação de atmosfera ligeiramente oxidante. A maioria das formulações propostas envolve o uso de misturas binárias, ternárias e até quaternárias dos gases Ar, He, CO2 e O2. Em aplicações específicas, adições de N2 e H2 foram usadas. Neste sentido, pouca inovação foi identificada, sendo atribuídas possíveis razões a este fato. A tendência observada para o desenvolvimento de gases de proteção parece ser o uso de misturas ricas em gases inertes, com adições calibradas de elementos oxidantes, sempre atendendo a aplicações específicas.<br>This paper has as objective to present a state of the art review on the shielding gases used in the electric arc welding processes, with emphasis on GMAW welding of steel. There are shown the main concepts of arc physics and properties related to shielding gases. The model of slightly oxidizing atmosphere for shielding gases is discussed, and the formulations of the gases studied in scientific articles and in deposited patents, as well as the claims (benefits) required. It also mentions some few studies using additions of non-conventional gases. It was observed that for the development of shielding gases for the GMAW steel welding the model of slightly oxidizing atmosphere is predominant. Most of proposed formulations involve the use of binary, ternary or even quaternary mixtures of the gases Ar, He, CO2 and O2. For specific aplications, additions of N2 e H2 were used. In this meaning, few innovations were identified, and possible reasons were attributed. The trend observed for the development of shielding gases seems to be the use of mixtures rich in inert gases, with calibrated additions of oxidizing elements, always looking for specific applications