High-speed Countercurrent Chromatography As A Tool To Isolate Nerolidol From The Baccharis Dracunculifolia Volatile Oil

Baccharis dracunculifolia is a shrub native to Brazil and an important botanical source for propolis production. The essential oil from the leaves of B. dracunculifolia DC (Asteraceae), popularly known as broom oil, presents pharmacological activities and is a raw material in the perfumery and cosmetics industries. The isolation of its major constituent, nerolidol, to a high purity (92.5-93.7%) was possible through the countercurrent chromatography technique. The countercurrent chromatography biphasic solvent system chosen was a mixture of hexane:methanol:water (5:4:1, v/v/v). Isocratic elution was conducted in a tail-to-head manner. © 2014 Taylor & Francis.265334337Queiroga, C.L., Fukai, A., Marsaioli, A.J., Three new oxygenated cadinanes from Baccharis species (1990) J. Braz. Chem. Soc., 1, pp. 105-109Pereira, C.A., Da Costa, A.C.B.P., Machado, A.K.S., Beltrame Junior, M., Zöllner, M.S.A.C., Junqueira, J.C., Enzymatic activity sensitivity to antifungal drugs and Baccharis dracunculifolia essential oil by Candida strains isolated from the oral cavities of breastfeeding infants (2011) Mycopathologia, 171, pp. 103-109Massignani, J.J., Lemos, M., Maistro, E.L., Schafhauser, H.P., Jorge, R.F., Sousa, J.P.B., Bastos, J.K., De Andrade, S.F., Antiulcerogenic activity of the essential oil of Baccharis dracunculifolia on differen t experimental models in rats (2009) Phytother. Res., 23, pp. 1355-1360Parreira, N.A., Magalhães, L.G., Moraes, D.R., Caixeta, S.C., De Sousa, J.P.B., Bastos, J.K., Cunha, W.R., Da Silva Filho, A.A., Antiprotozoal schistosomicidal, and antimicrobial activities of the essential oil from the leaves of Baccharis dracunculifolia (2007) Chem. Biodivers., 7, pp. 993-1001Queiroga, C.L., Bastos, J.K., De Souza, J.P.B., De Magalhães, P.M., Comparison of the chemical c omposition of the essential oil and the water soluble oil of Baccharis dracunculifolia DC. (Asteraceae) (2008) J. Essent. Oil Res., 20, pp. 111-114Maróstica Júnior, M.R., Daugsch, A., Moraes, C.S., Queiroga, C.L., Pastore, G.M., Park, Y.K., Comparison of volatile and polyphenolic compounds in Brazilian green propolis and its botanical origin Baccharis dracunculifolia (2008) Ciěncia e Tecnologia de Alimentos, 28, pp. 178-181Martinez-Correa, H.A., Cabral, F.A., Magalhães, P.M., Queiroga, C.L., Godoy, A.T., Sánchez-Camargo, A.P., Paviani, L.C., Extracts from the leaves of Baccharis dracunculifolia obtained by a combination of ex traction processes with supercritical CO2, ethanol and water (2012) J. Supercrit. Fluids, 63, pp. 31-39Bauer, K., Garbe, D., Surburg, H., (1990) Common Fragrance and Flavor Materials Prepa Ration, Properties, and Uses, , VCH Publishers, New York, NYMcGinty, D., Letizia, C.S., Api, A.M., Review-Addendum to Fragrance material review on nerolidol (isomer unspecified) (2010) Food Chem. Toxicol., 48, pp. S43-S45Xie, J., Wang, S., Sun, B., Yto, I., Isolation and purification of nootkatone from the essential oil of fruits of Alpinia oxyphylla Miquel by high-speed counter-current chromatography (2009) Food Chem., 117, pp. 375-380Adams, R.P., (1995) Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, , Allured Publishing Corp Carol Stream, I