598 research outputs found
Using PUF disk passive samplers to simultaneously measure air concentrations of persistent organic pollutants (POPs) across the Tuscany Region, Italy
Abstract Passive air samplers consisting of polyurethane foam (PUF) disks were deployed simultaneously over 2â4 month periods from April to July 2008 at 19 locations throughout the Tuscany Region at urban (n = 9), rural (n = 7) and agricultural sites (n = 3). The purpose of the study was to assess the gasâphase air concentrations of PCBs, OCPs, PBDEs and PAHs on a local and regional scale. Derived air concentrations (pg m â3 ) ranged from below detection limit (BDL) to 300 for ÎŁ 6 PCBs and from BDL to 30 for ÎŁ 3 PBDEs. As expected, the highest PCB concentrations (pg m â3 ) were observed at urban sites, peaking in Piombino (300). PBDEs, however, exhibited a "reverse gradient" (i.e. rural>agricultural>urban) with highest air concentration at the rural site, Arcidoso (30). Levels of OCPs showed less distinct differences among the three site types. The most frequently detected OCPs were Endosulfan I, ÎłâHCH and αâHCH. Endosulfan I ranged from BDL to 2 200 pg m â3 , αâHCH from BDL to 610, and ÎłâHCH from BDL to 1 100. The high air concentrations of HCHs were surprising and possibly due to continued and illegal use â especially at agricultural and urban sites that exhibited the highest levels in air. PAHs showed highest concentrations (pg m â3 ) at agricultural and urban sites, ranging from 290 to 4 700 and were generally consistent with other data sets from Europe. PAHs were dominated by the 3âring PAHs (76 ± 6)%, with phenanthrene alone accounting for (45 ± 11%) of the total PAHs. This study demonstrates the feasibility of using PUF disks as timeâintegrating passive air samplers for assessing local and regional scale concentrations of a wide range of POPs in central Italy. Given their low cost and simplicity, passive air samplers are the preferred option for conducting concurrent sampling at multiple sites for monitoring and research purposes
Using PUF disk passive samplers to simultaneously measure air concentrations of persistent organic pollutants (POPs) across the Tuscany Region, Italy
AbstractPassive air samplers consisting of polyurethane foam (PUF) disks were deployed simultaneously over 2â4 month periods from April to July 2008 at 19 locations throughout the Tuscany Region at urban (n=9), rural (n=7) and agricultural sites (n=3). The purpose of the study was to assess the gasâphase air concentrations of PCBs, OCPs, PBDEs and PAHs on a local and regional scale. Derived air concentrations (pg mâ3) ranged from below detection limit (BDL) to 300 for ÎŁ6PCBs and from BDL to 30 for ÎŁ3PBDEs. As expected, the highest PCB concentrations (pg mâ3) were observed at urban sites, peaking in Piombino (300). PBDEs, however, exhibited a âreverse gradientâ (i.e. rural>agricultural>urban) with highest air concentration at the rural site, Arcidoso (30). Levels of OCPs showed less distinct differences among the three site types. The most frequently detected OCPs were Endosulfan I, ÎłâHCH and αâHCH. Endosulfan I ranged from BDL to 2 200pg mâ3, αâHCH from BDL to 610, and ÎłâHCH from BDL to 1 100. The high air concentrations of HCHs were surprising and possibly due to continued and illegal use â especially at agricultural and urban sites that exhibited the highest levels in air. PAHs showed highest concentrations (pg mâ3) at agricultural and urban sites, ranging from 290 to 4 700 and were generally consistent with other data sets from Europe. PAHs were dominated by the 3âring PAHs (76±6)%, with phenanthrene alone accounting for (45±11%) of the total PAHs. This study demonstrates the feasibility of using PUF disks as timeâintegrating passive air samplers for assessing local and regional scale concentrations of a wide range of POPs in central Italy. Given their low cost and simplicity, passive air samplers are the preferred option for conducting concurrent sampling at multiple sites for monitoring and research purposes
Monitoring persistent organic chemicals in Antarctica in support of global chemical policy: a horizon scan of priority actions and challenges
Global production and emission of chemicals exceeds societal capacities for assessment and monitoring. This situation calls for improved chemical regulatory policy frameworks and increased support for expedited decision making within existing frameworks. The polar regions of the Earth represent unique sentinel areas for the study of global chemical behaviour, and data arising from these areas can strengthen existing policy frameworks. However, chemical pollution research and monitoring in the Antarctic is underdeveloped, with geopolitical complexities and the absence of legal recognition of international chemical policy serving to neutralise progress made in other global regions. This Personal View represents a horizon scan by the action group Input Pathways of Persistent Organic Pollutants to Antarctica, of the Scientific Committee for Antarctic Research. Four priority research and research facilitation gaps are outlined, with recommendations for Antarctica Treaty parties for strategic action against these priorities
The effect of four mutations on the expression of iduronate-2-sulfatase in mucopolysaccharidosis type II
AbstractMucopolysaccharidosis type II (Hunter syndrome; OMIM 309900) is a rare X-linked recessive lysosomal storage disorder caused by the deficiency of the enzyme iduronate-2-sulfatase (IDS; EC 3.1.6.13). Different alterations at the IDS locus, mostly missense mutations, have been demonstrated, by expression study, as deleterious, causing significant consequences on the enzyme function or stability. In the present study we report on the results of the transient expression of the novel K347T, 533delTT, N265I and the already described 473delTCC (previously named ÎS117) mutations in the COS 7 cells proving their functional consequence on IDS activity. This type of information is potentially useful for genotypeâphenotype correlation, prognosis and possible therapeutic intervention
Lipid and fatty acid composition, and persistent organic pollutant levels in tissues of migrating Atlantic bluefin tuna (Thunnus thynnus, L.) broodstock
Lipid class, fatty acid and POP levels were measured in migrating Atlantic bluefin tuna (ABT) tissues caught off the Barbate coast, Spain. Tissue lipids were largely characterized by triacylglycerol, reflecting large energy reserves accumulated prior to reproductive migration. Fatty acid compositions of muscle, liver and adipose exhibited similar profiles, whereas gonads showed a higher affinity for docosahex- aenoic acid. Tissue POP concentrations correlated positively with percentage triacylglycerol and nega- tively with polar lipids. Highest POP concentrations were in adipose and lowest in gonads, reflecting lipid content. DL-PCBs contributed most to total PCDD/F þ DL-PCB levels, with mono-ortho concentrations higher in tissues, whereas non-ortho PCBs contributed greater WHO-TEQs due to differences in TEFs. PBDE47 was the most prominent BDE congener in tissues, probably through biotransformation of BDE99 and other higher brominated congeners. The perceived POP risk from ABT consumption should be balanced by the well-established beneficial effects on human health of omega-3 fatty acids
A multidrug approach to modulate the mitochondrial metabolism impairment and relative oxidative stress in fanconi anemia complementation group a
Fanconi Anemia (FA) is a rare recessive genetic disorder characterized by aplastic anemia due to a defective DNA repair system. In addition, dysfunctional energy metabolism, lipid droplets accumulation, and unbalanced oxidative stress are involved in FA pathogenesis. Thus, to modulate the altered metabolism, Fanc-A lymphoblast cell lines were treated with quercetin, a flavonoid compound, C75 (4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylic acid), a fatty acid synthesis inhibitor, and rapamycin, an mTOR inhibitor, alone or in combination. As a control, isogenic FA cell lines corrected with the functional Fanc-A gene were used. Results showed that: (i) quercetin recovered the energy metabolism efficiency, reducing oxidative stress; (ii) C75 caused the lipid accumulation decrement and a slight oxidative stress reduction, without improving the energy metabolism; (iii) rapamycin reduced the aerobic metabolism and the oxidative stress, without increasing the energy status. In addition, all molecules reduce the accumulation of DNA double-strand breaks. Two-by-two combinations of the three drugs showed an additive effect compared with the action of the single molecule. Specifically, the quercetin/C75 combination appeared the most efficient in the mitochondrial and lipid metabolism improvement and in oxidative stress production reduction, while the quercetin/rapamycin combination seemed the most efficient in the DNA breaks decrement. Thus, data reported herein suggest that FA is a complex and multifactorial disease, and a multidrug strategy is necessary to correct the metabolic alterations
Further genotype-phenotype correlation emerging from two families with PLP1 exon 4 skipping
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