Variation in Lepidopteran Occurrence in Hemlock-Dominated and Deciduous-Dominated Forests of Central Appalachia

Eastern hemlock, (Tsuga canadensis Carrière, Pinaceae), is threatened with extirpation by an exotic invasive herbivore, the hemlock woolly adelgid, (Adelges tsugae Annand, Homoptera: Adelgidae). Given this threat, a broader and more detailed knowledge of the community associated with eastern hem- lock is merited. As Lepidoptera are important members of forest communities, this study was initiated to determine the relative occurrence of Lepidoptera in hemlock-dominated and deciduous-dominated habitats by evaluating abundance, species richness, temporal variation, and composition overlap. Lepidoptera were surveyed using blacklight traps from May – August 2010 at two collection sites in the Appalachian region of eastern Kentucky. The first collection site was within a forest stand dominated by mixed deciduous species, the second site possessed an overstory of eastern hemlock. Lepidoptera ≥ 20 mm in wingspan were identified and enumerated, yielding a total of 1,020 individuals of ≥ 137 species and 18 families. The total number of Lepidoptera captured in May and June was fewer than in July and August (P ≤ 0.05). The composition of the assemblage varied between collection sites as well as seasonally; 85 species were identified at the deciduous site and 107 species were identified at the hemlock site. While 27 species were recorded only at the deciduous site, 49 species were unique to the hemlock site. Of those unique to the hemlock site, five species were either detritivores or conifer specialists. These data demonstrate the importance of both deciduous and hemlock-dominated forest habitats for many species of Lepidoptera in Appalachia. Our study forms a foundation for understanding species richness patterns of Lepidoptera in hemlock forests in North America and is a useful baseline for comparisons of richness and diversity post invasion by the hemlock woolly adelgid

Do Tactile Activities During Good Citizenship Lessons Minimize Misbehavior More Than Activities Using Traditional Strategies?

https://scholarworks.moreheadstate.edu/student_scholarship_posters/1195/thumbnail.jp

Variation in Lepidopteran Occurrence in Hemlock-Dominated and Deciduous-Dominated Forests of Central Appalachia

Eastern hemlock, (Tsuga canadensis Carrière, Pinaceae), is threatened with extirpation by an exotic invasive herbivore, the hemlock woolly adelgid, (Adelges tsugae Annand, Homoptera: Adelgidae). Given this threat, a broader and more detailed knowledge of the community associated with eastern hem- lock is merited. As Lepidoptera are important members of forest communities, this study was initiated to determine the relative occurrence of Lepidoptera in hemlock-dominated and deciduous-dominated habitats by evaluating abundance, species richness, temporal variation, and composition overlap. Lepidoptera were surveyed using blacklight traps from May – August 2010 at two collection sites in the Appalachian region of eastern Kentucky. The first collection site was within a forest stand dominated by mixed deciduous species, the second site possessed an overstory of eastern hemlock. Lepidoptera ≥ 20 mm in wingspan were identified and enumerated, yielding a total of 1,020 individuals of ≥ 137 species and 18 families. The total number of Lepidoptera captured in May and June was fewer than in July and August (P ≤ 0.05). The composition of the assemblage varied between collection sites as well as seasonally; 85 species were identified at the deciduous site and 107 species were identified at the hemlock site. While 27 species were recorded only at the deciduous site, 49 species were unique to the hemlock site. Of those unique to the hemlock site, five species were either detritivores or conifer specialists. These data demonstrate the importance of both deciduous and hemlock-dominated forest habitats for many species of Lepidoptera in Appalachia. Our study forms a foundation for understanding species richness patterns of Lepidoptera in hemlock forests in North America and is a useful baseline for comparisons of richness and diversity post invasion by the hemlock woolly adelgid

Discovery of Strong Radiative Recombination Continua from The Supernova Remnant IC 443 with Suzaku

We present the Suzaku spectroscopic study of the Galactic middle-aged supernova remnant (SNR) IC 443. The X-ray spectrum in the 1.75-6.0 keV band is described by an optically-thin thermal plasma with the electron temperature of 0.6 keV and several additional Lyman lines. We robustly detect, for the first time, strong radiative recombination continua (RRC) of H-like Si and S around at 2.7 and 3.5 keV. The ionization temperatures of Si and S determined from the intensity ratios of the RRC to He-like K-alpha line are 1.0 keV and 1.2 keV, respectively. We thus find firm evidence for an extremely-overionized (recombining) plasma. As the origin of the overionization, a thermal conduction scenario argued in previous work is not favored in our new results. We propose that the highly-ionized gas were made at the initial phase of the SNR evolution in dense regions around a massive progenitor, and the low electron temperature is due to a rapid cooling by an adiabatic expansion.Comment: 5 pages, 5 figures, accepted by ApJ Lette

XMM-Newton observations of the supernova remnant IC443: I. soft X-ray emission from shocked interstellar medium

The shocked interstellar medium around IC443 produces strong X-ray emission in the soft energy band (E<1.5 keV). We present an analysis of such emission as observed with the EPIC MOS cameras on board the XMM-Newotn observatory, with the purpose to find clear signatures of the interactions with the interstellar medium (ISM) in the X-ray band, which may complement results obtained in other wavelenghts. We found that the giant molecular cloud mapped in CO emission is located in the foreground and gives an evident signature in the absorption of X-rays. This cloud may have a torus shape and the part of torus interacting with the IC443 shock gives rise to 2MASS-K emission in the southeast. The measured density of emitting X-ray shocked plasma increases toward the northeastern limb, where the remnant is interacting with an atomic cloud. We found an excellent correlation between emission in the 0.3-0.5 keV band and bright optical/radio filament on large spatial scales. The partial shell structure seen in this band therefore traces the encounter with the atomic cloud.Comment: 10 pages, 10 figures, accepted for publication in ApJ (20 September 2006, v649). For hi-res figures, see http://www.astropa.unipa.it/Library/OAPA_preprints/ic443ele1.ps.g

piggyBac Transposon Somatic Mutagenesis with an Activated Reporter and Tracker (PB-SMART) for Genetic Screens in Mice

Somatic forward genetic screens have the power to interrogate thousands of genes in a single animal. Retroviral and transposon mutagenesis systems in mice have been designed and deployed in somatic tissues for surveying hematopoietic and solid tumor formation. In the context of cancer, the ability to visually mark mutant cells would present tremendous advantages for identifying tumor formation, monitoring tumor growth over time, and tracking tumor infiltrations and metastases into wild-type tissues. Furthermore, locating mutant clones is a prerequisite for screening and analyzing most other somatic phenotypes. For this purpose, we developed a system using the piggyBac (PB) transposon for somatic mutagenesis with an activated reporter and tracker, called PB-SMART. The PB-SMART mouse genetic screening system can simultaneously induce somatic mutations and mark mutated cells using bioluminescence or fluorescence. The marking of mutant cells enable analyses that are not possible with current somatic mutagenesis systems, such as tracking cell proliferation and tumor growth, detecting tumor cell infiltrations, and reporting tissue mutagenesis levels by a simple ex vivo visual readout. We demonstrate that PB-SMART is highly mutagenic, capable of tumor induction with low copy transposons, which facilitates the mapping and identification of causative insertions. We further integrated a conditional transposase with the PB-SMART system, permitting tissue-specific mutagenesis with a single cross to any available Cre line. Targeting the germline, the system could also be used to conduct F1 screens. With these features, PB-SMART provides an integrated platform for individual investigators to harness the power of somatic mutagenesis and phenotypic screens to decipher the genetic basis of mammalian biology and disease

Enhanced β(2)-adrenergic receptor (β(2)AR) signaling by adeno-associated viral (AAV)-mediated gene transfer

BACKGROUND: β(2)-Adrenergic receptors (β(2)AR) play important regulatory roles in a variety of cells and organ systems and are important therapeutic targets in the treatment of airway and cardiovascular disease. Prolonged use of β-agonists results in tolerance secondary to receptor down-regulation resulting in reduced therapeutic efficiency. The purpose of this work is to evaluate the signaling capabilities of the β(2)AR expressed by a recombinant adeno-associated viral (AAV) vector that also included an enhanced green fluorescent protein (EGFP) gene (AAV-β(2)AR/EGFP). RESULTS: By epifluorescence microscopy, ~40% of infected HEK 293 cells demonstrated EGFP expression. β(2)AR density measured with [(3)H]dihydroalprenolol ([(3)H]DHA) increased either 13- or 77-fold in infected cells compared to mock infected controls depending on the culture conditions used. The [(3)H]DHA binding was to a single receptor population with a dissociation constant of 0.42 nM, as would be expected for wild-type β(2)AR. Agonist competition assays with [(3)H]DHA showed the following rank order of potency: isoproterenol>epinephrine> norepinephrine, consistent with β(2)AR interaction. Isoproterenol-stimulated cyclic AMP levels were 5-fold higher in infected cells compared to controls (314 ± 43 vs. 63.4 ± 9.6 nmol/dish; n = 3). Receptor trafficking demonstrated surface expression of β(2)AR with vehicle treatment and internalization following isoproterenol treatment. CONCLUSIONS: We conclude that HEK 293 cells infected with AAV-β(2)AR/EGFP effectively express β(2)AR and that increased expression of these receptors results in enhanced β(2)AR signaling. This method of gene transfer may provide an important means to enhance function in in vivo systems

ASCA Observations of the Supernova Remnant IC 443: Thermal Structure and Detection of Overionized Plasma

We present the results of X-ray spatial and spectral studies of the ``mixed-morphology'' supernova remnant IC 443 using ASCA. IC 443 has a center-filled image in X-ray band, contrasting with the shell-like appearance in radio and optical bands. The overall X-ray emission is thermal, not from a synchrotron nebula. ASCA observed IC 443 three times, covering the whole remnant. From the image analysis, we found that the softness-ratio map reveals a shell-like structure. At the same time, its spectra require two (1.0 keV and 0.2 keV) plasma components; the emission of the 0.2 keV plasma is stronger in the region near the shell than the center. These results can be explained by a simple model that IC 443 has a hot (1.0 keV) interior surrounded by a cool (0.2 keV) outer shell. From the emission measures, we infer that the 0.2 keV plasma is denser than the 1.0 keV plasma, suggesting pressure equilibrium between the two. In addition, we found that the ionization temperature of sulfur, obtained from H-like K$\alpha$ to He-like K$\alpha$ intensity ratio, is 1.5 keV, significantly higher than the gas temperature of 1.0 keV suggested from the continuum spectrum. The same can be concluded for silicon. Neither an additional, hotter plasma component nor a multi-temperature plasma successfully accounts for this ratio, and we conclude that the 1.0 keV plasma is overionized. This is the first time that overionized gas has been detected in a SNR. For the gas to become overionized in the absence of a photoionizing flux, it must cool faster than the ions recombine. Thermal conduction from the 1.0 keV plasma to the 0.2 keV one could cause the 1.0 keV plasma to become overionized, which is plausible within an old (3$\times10^4$ yr) SNR.Comment: 11 pages, 15 figures, 2 tables, accepted for publication in The Astrophysical Journa

The Ultraviolet Imaging Telescope: Instrument and Data Characteristics

The Ultraviolet Imaging Telescope (UIT) was flown as part of the Astro observatory on the Space Shuttle Columbia in December 1990 and again on the Space Shuttle Endeavor in March 1995. Ultraviolet (1200-3300 Angstroms) images of a variety of astronomical objects, with a 40 arcmin field of view and a resolution of about 3 arcsec, were recorded on photographic film. The data recorded during the first flight are available to the astronomical community through the National Space Science Data Center (NSSDC); the data recorded during the second flight will soon be available as well. This paper discusses in detail the design, operation, data reduction, and calibration of UIT, providing the user of the data with information for understanding and using the data. It also provides guidelines for analyzing other astronomical imagery made with image intensifiers and photographic film.Comment: 44 pages, LaTeX, AAS preprint style and EPSF macros, accepted by PAS

Prediction of photoperiodic regulators from quantitative gene circuit models

Photoperiod sensors allow physiological adaptation to the changing seasons. The external coincidence hypothesis postulates that a light-responsive regulator is modulated by a circadian rhythm. Sufficient data are available to test this quantitatively in plants, though not yet in animals. In Arabidopsis, the clock-regulated genes CONSTANS (CO) and FLAVIN, KELCH, F-BOX (FKF1) and their lightsensitive proteins are thought to form an external coincidence sensor. We use 40 timeseries of molecular data to model the integration of light and timing information by CO, its target gene FLOWERING LOCUS T (FT), and the circadian clock. Among other predictions, the models show that FKF1 activates FT. We demonstrate experimentally that this effect is independent of the known activation of CO by FKF1, thus we locate a major, novel controller of photoperiodism. External coincidence is part of a complex photoperiod sensor: modelling makes this complexity explicit and may thus contribute to crop improvement