Neuromuscular fatigue and endocrine responses in elite Australian Rules football players

The first purpose of this research was to establish the reliability of numerous measures obtained from a single and short duration repeated countermovement jump (CMJ) utilising a portable forceplate (Experimental Studies 1 and 2). Secondly, the response of reliable CMJ variables and T, C and T:C to a single elite level ARF match was assessed to identify the pattern of response and highlight those measures with the greatest potential for usefulness as monitoring tools across longer periods (Experimental Study 3). Finally, those variables identified as most valuable in Experimental Study 3 in addition to T, C and T:C; were measured throughout a season of elite ARF competition in order to examine the manner of their response and assess the magnitude of change in these variables in relation to performance and training and competition loads (Experimental Study 4)

Specificity and transfer of lower-body strength: influence of bilateral or unilateral lower-body resistance training

Appleby, BB, Cormack, SJ, and Newton, RU. Specificity and transfer of lower-body strength: Influence of bilateral or unilateral lower-body resistance training. J Strength Cond Res 33(2): 318-326, 2019-To examine the development of lower-body strength using either bilateral or unilateral resistance training. Developmental rugby players (n = 33; mean training age = 5.4 ± 2.9 years; 1 repetition maximum [1RM] 90° squat = 178 ± 27 kg) completed an 18-week randomized controlled training design (bilateral group [BIL], n = 13; unilateral group [UNI], n = 10; comparison, n = 10). The 8-week training phase involved 2 lower-body, volume-load matched resistance sessions per week (6-8 sets × 4-8 reps at 45-88% 1RM), differing only in the prescription of a bilateral (back squat) or unilateral (step-up) resistance exercise. Maximum strength was assessed by a randomized order of 1RM back squat and step-up testing and analyzed for within- and between-group differences using effect sizes (ES ± 90% confidence limits [CL]). Both training groups showed practically important improvements in their trained exercise (ES ± 90% CL: BIL = 0.67 ± 0.48; UNI = 0.74 ± 0.38) with transfer to their nontrained resistance exercise (BIL step-up = 0.27 ± 0.39: UNI squat = 0.42 ± 0.39). The difference between groups in adaptation of squat strength was unclear (BIL ES = -0.34 ± 0.55), while the UNI group showed an advantage in step-up training (ES = 0.41 ± 0.36). The results demonstrate that practically important increases in lower-body strength can be achieved using bilateral or unilateral resistance training and development of that strength may be expressed in the movement not trained, supporting the transfer of strength training between exercises of similar joint movements and muscles. Coaches may choose to incorporate unilateral strength training where the prescription of bilateral training may be inhibited

Kinetics and kinematics of the squat and step-up in well-trained rugby players

The purpose of this study was to compare and contrast the kinetics and kinematics of squat and step-up performance in well-trained athletes. Triaxial ground reaction force (GRF) and 3D kinematic data were collected in 4 maximal effort repetitions each at 70, 80, and 90% of 1 repetition maximum (1RM) of squat and step-up. The difference in concentric phase kinetics and kinematics between the squat and step-up was compared using effect sizes (ES ± 90% confidence limits [CLs]) classified as: less than 0.2 as trivial; 0.2-0.6 as small; 0.6-1.2 as moderate; and 1.2-2.0 as large. Where the 90% CL crossed negative and positive 0.2 values, the effect was considered unclear.n Ground reaction force was higher for the step-up than squat at all relative intensities per leg (peak GRF ES: 2.56 ± 0.19 to 2.70 ± 0.37; average GRF ES: 1.45 ± 0.27 to 1.48 ± 0.29). Per leg, the difference in concentric impulse favored the step-up compared with squat at 70% 1RM (ES = 0.71 ± 0.40) and 80% 1RM (ES = 0.30 ± 0.41) but was unclear at 90% 1RM (ES = -0.25 ± 0.47). The squat peak velocity was greater compared with step-up at all intensities (ES = -1.74 ± 0.48 to -1.33 ± 0.48). Despite a lower external load and a single base of support, per leg, the step-up produced comparable GRF because the squat suggesting overload provided by the step-up is sufficient for maximal strength development. Future research may investigate the efficacy of the step-up in a training intervention for the development of lower-body strength

Unilateral and bilateral lower-body resistance training does not transfer equally to sprint and change of direction performance

Given maximal strength can be developed using bilateral or unilateral resistance training, the purpose of this study was to determine the magnitude of transfer of unilateral or bilateral resistance training to sprint and change of direction (COD) performance. Thirty-three trained participants (average training age = 5.4 ± 2.9 years and 1 repetition maximum [1RM] 90° squat = 177.6 ± 26.7 kg) completed either a bilateral group (BIL, n = 13), unilateral (UNI, n = 10), or comparison (COM, n = 10) 18-week randomized controlled training design. Training involved 2 lower-body, volume-load-matched resistance sessions per week (6-8 sets × 4-8 reps at 45-88% 1RM), differing only in the prescription of a bilateral (squat) or unilateral (step-up) resistance exercise. Strength was assessed through 1RM squat and step-up, in addition to 20-m sprint and a customized 50° COD test. The effect size statistic ± 90% confidence limit (ES ± CL) was calculated to examine the magnitude of difference within and between groups at each time point. BIL and UNI groups improved their trained and nontrained strength exercise with an unclear difference in adaptation of squat strength (ES = -0.34 + 0.55). Both groups improved 20-m sprint (ES: BIL = -0.38 ± 0.49 and UNI = -0.31 ± 0.31); however, the difference between the groups was unclear (ES = 0.07 ± 0.58). Although both groups had meaningful improvements in COD performance, bilateral resistance training had a greater transfer to COD performance than unilateral resistance training (between-groups ES = 0.59 ± 0.64). Both bilateral and unilateral training improved maximal lower-body strength and sprint acceleration. However, the BIL group demonstrated superior improvements in COD performance. This finding potentially highlights the importance of targeting the underlying physiological stimulus that drives adaptation and not exercise selection based on movement specificity of the target performance

Reliability of squat kinetics in well-trained rugby players: Implications for monitoring training

The aim of this study was to determine the within-session reliability in kinetic variables of the squat in well-trained athletes during a typical resistance training protocol. Fifteen subjects completed 2 testing sessions. Session 1 was establishment of 1 repetition maximum (1RM) squat, and session 2 involved 2 sets of 2 maximal effort repetitions of the squat at 70, 80, and 90% of 1RM with 3D motion analysis and ground reaction force (GRF) measurement using 2 in-ground triaxial force plates. Reliability was calculated using typical error ± 90% confidence limits, expressed as the coefficient of variation and intraclass correlation coefficient. The smallest worthwhile change (SWC%), calculated as 0.2 × between-subject SD, was used to determine the smallest important change in performance. Peak GRF and average GRF were found to have acceptable measures of reliability with the combined left and right leg average GRF capable of detecting the SWC. Independent limb contributions were reliable (left and right, or dominant and nondominant). Reliable kinetics can be obtained in back squat performance typical of a resistance training session in well-trained athletes. This suggests that coaches integrating force plate technology within training sessions may effectively capture between 1 and 6 training sets among several athletes, facilitating analysis and intervention on larger data sets

The influence of different length between match microcycles on neuromuscular, hormonal and perceptual responses in professional rugby league players

Optimal recovery between matches is critically important in team sports. Indeed, altered neuromuscular performance, hormone levels and increased fatigue can manifest in under recovered team sport athletes. However, the recovery patterns in these variables in the days following professional rugby league matches is unknown. The purpose of this study was to examine the influence of different between-match training periods on neuromuscular, hormonal and perceptual measures in professional rugby league players. Following familiarization, 12 professional rugby league players from the same team were assessed for changes in countermovement jump (CMJ) (force, power, flight time), perceptual responses (fatigue, well being and muscle soreness) and salivary hormone (testosterone (T) and cortisol (C)) levels during 5, 7 and 9 day periods between matches. Measures were taken 4h prior to the initial match, and then 1, 2, 4, 6*, 7*, 8* and 9* days following (*depending on group). All training was prescribed by the coaches and monitored using the session-RPE method. Lower daily training load was completed on the 5-day compared with the 7 and 9-day conditions (p\u3c0.05). Some CMJ variables (flight time and flight time:contraction time), perception of fatigue, well-being and muscle soreness were reduced in the 2 days following the match in each condition (p\u3c0.05). All CMJ variables returned to near baseline values following 4 days in each condition. Both CMJ maximum and relative power were lower in the 7 day condition when compared with the 9 day condition (p\u3c0.05). Fatigue levels were reduced at 48 h in the 7 and 9-day groups (p\u3c0.05) but had returned to baseline in the 5-day group. All perceptual measures returned to baseline levels prior to the following match in each condition and followed changes in training load. Salivary T and C did not change in response to the match. These results show that neuromuscular performance and perception of fatigue are reduced for at least 48 h following a rugby league match but these can be fully recovered within 4 days. CMJ variables involving flight time appear to be best for assessing acute fatigue caused by a match, and variables involving power may be useful in assessing accumulated fatigue. The perceptual measures were sensitive to both acute fatigue and accumulated fatigue. The salivary hormone analyses were of limited value for assessing recovery. These findings show that with correct training, it is possible to recover neuromuscular, perceptual and endocrine measures within 4 days after a rugby league match

Continuous macroscopic limit of a discrete stochastic model for interaction of living cells

In the development of multiscale biological models it is crucial to establish a connection between discrete microscopic or mesoscopic stochastic models and macroscopic continuous descriptions based on cellular density. In this paper a continuous limit of a two-dimensional Cellular Potts Model (CPM) with excluded volume is derived, describing cells moving in a medium and reacting to each other through both direct contact and long range chemotaxis. The continuous macroscopic model is obtained as a Fokker-Planck equation describing evolution of the cell probability density function. All coefficients of the general macroscopic model are derived from parameters of the CPM and a very good agreement is demonstrated between CPM Monte Carlo simulations and numerical solution of the macroscopic model. It is also shown that in the absence of contact cell-cell interactions, the obtained model reduces to the classical macroscopic Keller-Segel model. General multiscale approach is demonstrated by simulating spongy bone formation from loosely packed mesenchyme via the intramembranous route suggesting that self-organizing physical mechanisms can account for this developmental process.Comment: 4 pages, 3 figure

Sleep regularity and predictors of sleep efficiency and sleep duration in elite team sport athletes

Background Many elite athletes have suboptimal sleep duration and efficiency, potentially due to factors that may impact sleep onset and offset times. Variability in sleep onset and offset may negatively influence sleep. The sleep regularity index (SRI) is a novel metric for sleep regularity, however there are no published descriptions of SRI in elite athletes. Further, contributors to sleep efficiency and duration in elite athletes using objective measures have not been explored. Methods Sleep was monitored over a minimum of seven consecutive days (7 to 43)—in 203 elite team sport athletes (age range = 19–36 years; female, n = 79; male, n = 124, total sleep nights = 1975) using activity monitoring and sleep diaries. The sleep regularity index (SRI) was calculated to reflect the night-to-night shifts in sleep by accounting for changes in sleep onset and sleep offset. Sleep characteristics were compared between regular and irregular sleepers and important contributors to sleep efficiency and total sleep time were assessed using multiple linear regression models. Results The median sleep regularity index and interquartile range were 85.1 (81.4 to 88.8). When compared to irregular sleepers, regular sleepers demonstrated (1) significantly greater sleep efficiency (p = 0.006; 0.31 medium effect size [ES]), (2) significantly less variability in total sleep time (− p ≤ 0.001; − 0.69, large ES) and sleep efficiency (− 0.34, small ES), (3) similar total sleep time and (4) significantly less variation in sleep onset (p ≤ 0.001; − 0.73, large ES) and offset (p ≤ 0.001; − 0.74, large ES) times. Sleep characteristics explained 73% and 22% of the variance in total sleep time and sleep efficiency, respectively. The most important contributor to total sleep time was a later sleep offset time, while the most important contributors to sleep efficiency were an earlier bedtime and less variable sleep onset times. Conclusions Bedtime and a consistent sleep onset time are important factors associated with sleep efficiency in athletes, while sleep offset is an important factor for total sleep time. Coaches and staff can assist their athletes by providing training schedules that allow for both regularity and sufficiency of time in bed where possible

Invasive versus medical management in patients with prior coronary artery bypass surgery with a non-ST segment elevation acute coronary syndrome: a pilot randomized controlled trial

Background: The benefits of routine invasive management in patients with prior coronary artery bypass grafts presenting with non-ST elevation acute coronary syndromes are uncertain because these patients were excluded from pivotal trials. Methods: In a multicenter trial, non-ST elevation acute coronary syndromes patients with prior coronary artery bypass graft were prospectively screened in 4 acute hospitals. Medically stabilized patients were randomized to invasive management (invasive group) or noninvasive management (medical group). The primary outcome was adherence with the randomized strategy by 30 days. A blinded, independent Clinical Event Committee adjudicated predefined composite outcomes for efficacy (all-cause mortality, rehospitalization for refractory ischemia/angina, myocardial infarction, hospitalization because of heart failure) and safety (major bleeding, stroke, procedure-related myocardial infarction, and worsening renal function). Results: Two hundred seventeen patients were screened and 60 (mean±SD age, 71±9 years, 72% male) were randomized (invasive group, n=31; medical group, n=29). One-third (n=10) of the participants in the invasive group initially received percutaneous coronary intervention. In the medical group, 1 participant crossed over to invasive management on day 30 but percutaneous coronary intervention was not performed. During 2-years’ follow-up (median [interquartile range], 744 [570–853] days), the composite outcome for efficacy occurred in 13 (42%) subjects in the invasive group and 13 (45%) subjects in the medical group. The composite safety outcome occurred in 8 (26%) subjects in the invasive group and 9 (31%) subjects in the medical group. An efficacy or safety outcome occurred in 17 (55%) subjects in the invasive group and 16 (55%) subjects in the medical group. Health status (EuroQol 5 Dimensions) and angina class in each group were similar at 12 months. Conclusions: More than half of the population experienced a serious adverse event. An initial noninvasive management strategy is feasible. A substantive health outcomes trial of invasive versus noninvasive management in non-ST elevation acute coronary syndromes patients with prior coronary artery bypass grafts appears warranted. Clinical Trial Registration: URL: https://www.clinicaltrials.gov. Unique identifier: NCT01895751

Mutation of RNA Pol III Subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and Disrupts Zebrafish Digestive Development

The role of RNA polymerase III (Pol III) in developing vertebrates has not been examined. Here, we identify a causative mutation of the second largest Pol III subunit, polr3b, that disrupts digestive organ development in zebrafish slim jim (slj) mutants. The slj mutation is a splice-site substitution that causes deletion of a conserved tract of 41 amino acids in the Polr3b protein. Structural considerations predict that the slj Pol3rb deletion might impair its interaction with Polr3k, the ortholog of an essential yeast Pol III subunit, Rpc11, which promotes RNA cleavage and Pol III recycling. We engineered Schizosaccharomyces pombe to carry an Rpc2 deletion comparable to the slj mutation and found that the Pol III recovered from this rpc2-Δ yeast had markedly reduced levels of Rpc11p. Remarkably, overexpression of cDNA encoding the zebrafish rpc11 ortholog, polr3k, rescued the exocrine defects in slj mutants, indicating that the slj phenotype is due to deficiency of Rpc11. These data show that functional interactions between Pol III subunits have been conserved during eukaryotic evolution and support the utility of zebrafish as a model vertebrate for analysis of Pol III function