Caracterización molecular de nanoportadores de fármacos mediante espectroscopía intensificada por plasmones localizados: Fluorescencia (SEF) y Raman (SERS)

The combined application of SERS (Surface-Enhanced Raman Scattering) and SEF (Surface Enhanced Fluorescence) to the molecular characterization of druǵs nanocarriers based on silver colloids is here shown for the antitumoral drug emodin, and the Non-Steroidal Anti-Inflammatory Drugs indomethacin and ketorolac. The system >silver nanoparticles-emodin> was subsequently embedded in porous silicon and the new system also characterized. The joint analysis of the SERS, SEF and UV-Vis spectra of indomethacin and ketorolac at different pHs has also allowed to propose schemes for the approaching mechanisms of the drug molecules to the silver nanoparticles surface in the pH range studied.© Sociedad Española de Óptica.Se agradece al MINECO (proyecto FIS2010-15405), a la Comunidad de Madrid (proyecto MICROSERES II-S 2009TIC-1476) y al Grupo Investigación 950247 de la UCM, la financiación recibida para llevar a cabo este trabajo.Peer Reviewe

Noble metal nanoparticles used as carriers systems for NSAIDs drugs: SEF, release and binding

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Site specific replacements of a single loop nucleoside with a dibenzyl linker may switch the activity of TBA from anticoagulant to antiproliferative

Many antiproliferative G-quadruplexes (G4s) arise from the folding of GT-rich strands. Among these, the Thrombin Binding Aptamer (TBA), as a rare example, adopts a monomolecular well-defined G4 structure. Nevertheless, the potential anticancer properties of TBA are severely hampered by its anticoagulant action and, consequently, no related studies have appeared so far in the literature. We wish to report here that suitable chemical modifications in the TBA sequence can preserve its antiproliferative over anticoagulant activity. Particularly, we replaced one residue of the TT or TGT loops with a dibenzyl linker to develop seven new quadruplex-forming TBA based sequences (TBA-bs), which were studied for their structural (CD, CD melting, 1D NMR) and biological (fibrinogen, PT and MTT assays) properties. The three-dimensional structures of the TBA-bs modified at T13 (TBA-bs13) or T12 (TBA-bs12), the former endowed with selective antiproliferative activity, and the latter acting as potently as TBA in both coagulation and MTT assays, were further studied by 2D NMR restrained molecular mechanics. The comparative structural analyses indicated that neither the stability, nor the topology of the G4s, but the different localization of the two benzene rings of the linker was responsible for the loss of the antithrombin activity for TBA-bs13

Molecular characterization of drug nanocarriers based on Plasmon Enhanced Spectroscopies: Fluorescence (SEF) and Raman (SERS)

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Host-Guest complexes of drugs emodin, piroxicam and indomethacin with cucurbiturils: UV-visible, fluorescence, 1H NMR and Raman spectroscopic study

ConferenciaSpanish MINECO (Project FIS2010-15405), Comunidad de Madrid (Project MICROSERES II-S2009TIC-1476) and Grupo Investigacion 950247 of the UCM are gratefully acknowledged for financial support.Peer Reviewe

Characterization of supramolecular complexes formed between non-steroidal anti-inflamatory drugs and cucurbit[n]urils adsorbed on silver nanoparticles

Escuela Técnica Superior de Ingenieros Industriales (ETSII - UPM), Madrid (Spain), March 11-14 (2014)Peer Reviewe

Fluorescence of non-steroidal anti-inflammatory drugs ketorolac and indomethacin: water solution and silver colloid

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Caracterización molecular mediante SEF (Surface Enhanced Fuorescence) y SERS (Surface Enhanced Raman Scattering) de sistemas nanotransportadores de los fármacos emodina, ketorolac e indometacina

Contribution presented at XVIII Congreso Argentino de Fisicoquímica y Química Inorgánica,held in Rosario, Argentina de 9 al 12 de abril de 2013. More details at http://aaifq.org.ar/libroCafqi2013/index.php?controller=render&action=home.Peer Reviewe

Molecular characterization of drug nanocarriers based on plasmon enhanced spectroscopies: fluorescence (SEF) and Raman (SERS)

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SERS and SEF study of Piroxicam in silver and gold colloids

Peer Reviewe