Etude du couple facteur Willebrand/Adamts13 dans un modèle de thrombose de la microcirculation

La drépanocytose est considérée comme un état permanent d hypercoagulabilité. Si les phénomènes d adhérence à l endothélium vasculaire impliquent les GR, en particulier la population réticulocytaire, ils impliquent aussi les leucocytes et des protéines plasmatiques normalement présentes ou libérées par les plaquettes et l endothélium, telles la thrombospondine ou le facteur Willebrand (VWF). En effet, la littérature rapporte une augmentation des taux de VWF participant à l hyperadhésivité des globules rouges (GR) drépanocytaires à l endothélium activé, par le biais des multimères de très haut poids moléculaire (THPM) du VWF. Dans certains cas très sévères de crises vaso-occlusives (CVO), la présentation clinico-biologique peut rappeler celle du purpura thrombotique thrombocytopénique (PTT), forme particulière de microangiopathie thrombotique (MAT), définie par la triade : thrombopénie de consommation, anémie hémolytique mécanique et signes d ischémie multiviscérale. Dans le PTT, il existe un déficit fonctionnel sévère en ADAMTS13 (A Disintegrin-like And Metalloprotease with ThromboSpondin type 1 repeats), cette métalloprotéase ayant pour fonction principale de limiter la taille (et donc le pouvoir adhésif vis-à-vis des plaquettes) des multimères du VWF.CHATENAY M.-PARIS 11-BU Pharma. (920192101) / SudocSudocFranceF

Influence of Post-Milking Treatment on Microbial Diversity on the Cow Teat Skin and in Milk

International audienceIn dairy cattle, teat disinfection at the end of milking is commonly applied to limit colonization of the milk by pathogenic microorganisms via the teat canal. The post-milking products used can irritate the teat skin and unbalance its microbial population. Our study aimed to assess the impact of different milking products on the balance of the microbial communities on the teat skin of cows and in their milk. For 12 weeks at the end of each milking operation, three groups of seven Holstein dairy cows on pasture received either a chlorhexidine gluconate-based product (G) or a hydrocolloidal water-in-oil emulsion (A), or no post-milking product (C). The composition of the bacterial and fungal communities on the teat skin and in the milk were characterized using a culture-dependent method and by high-throughput sequencing of marker genes to obtain amplicon sequence variants (ASVs). The individual microbiota on the cows' teat skin was compared for the first time to that of a cow pool. In contrast to the milk, the post-milking treatment influenced the microbiota of the teat skin, which revealed a high microbial diversity. The water-in-oil emulsion appeared to slightly favour lactic acid bacteria and yeasts and to limit the development of undesirable bacteria such as Pseudomonas and Staphylococcus

The feminine affective universe in Vernon Subutex of Virginie Despentes

Ever since the publication of her first novel Baise-moi (1993), gender identities have constituted a leitmotivin Virginie Despentes’ work. Vernon Subutex, her latest novel published as a trilogy from 2015 to 2017, pursues this exploration. The three volumes portray intermittently twenty-six characters and, in addition to their social experiences, Despentes exposes us to their interiority as, in her fiction, the social can never be divorced from the personal. At first glance, the novel seems to reinforce the gender-based division of the univers affectifs. The novel actually reveals how the gendered dichotomy assigning reason and the public sphere to men and binding women to emotions and the private sphere still shapes identities. Women in particular seem mostly preoccupied with personal relationships, just as they are reduced to their gender, if not sex: love and their (self-)objectification prevail. Despentes does not explicitly denounce the persistence of gender-based ideals or of the gendering of the univers affectifs. On the contrary, the faithful rendition of the female characters’ interiority raises the readers’ awareness as to this phenomenon. The critique occurs, therefore, on the metadiscursive level