Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice-9

<p><b>Copyright information:</b></p><p>Taken from "Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice"</p><p>BMC Genomics 2007;8():151-151.</p><p>Published online 7 Jun 2007</p><p>PMCID:PMC1914052.</p><p></p>) and achieves maximal induction of approximately 5-fold following three daily doses (3 × 24 hrs) of 100 μg/kg TAM. Significant increases (p < 0.05, n = 5) are denoted by an asterisk (*). In contrast, 100 μg/kg EE (positive control) maximally induced uterine wet weight 11-fold (*, p < 0.05, n = 5) with significant water imbibition (#; p < 0.05, n = 3), while TAM only achieved 50% uterotrophic efficacy and no water imbibition. B) A single dose of 100 μg/kg TAM significantly increased uterine wet weight as early as 24 hrs after administration. No significant water imbibition was observed at any time point

Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice-4

<p><b>Copyright information:</b></p><p>Taken from "Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice"</p><p>BMC Genomics 2007;8():151-151.</p><p>Published online 7 Jun 2007</p><p>PMCID:PMC1914052.</p><p></p>lear staining, indicating greater levels of Pcna protein expression, in agreement with the histological assessment and changes in gene expression associated with cell proliferation. Increased Pcna expression is more pronounced in the luminal and glandular epithelium, and stroma (arrows). Tissues were counter-stained with hematoxylin. Images are representative of four biological replicates. Bars represent 20 μm

Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice-5

<p><b>Copyright information:</b></p><p>Taken from "Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice"</p><p>BMC Genomics 2007;8():151-151.</p><p>Published online 7 Jun 2007</p><p>PMCID:PMC1914052.</p><p></p>ndicates that early responses (4 hrs) to ethynylestradiol (E) are most similar to 8 and 12 hrs tamoxifen (T) responses demonstrating temporally displaced TAM activation consistent with the delayed absorption of TAM. However, temporal displacement of TAM elicited responses is not maintained as EE and TAM responses cluster together at 24 and 72 hrs

Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice-0

<p><b>Copyright information:</b></p><p>Taken from "Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice"</p><p>BMC Genomics 2007;8():151-151.</p><p>Published online 7 Jun 2007</p><p>PMCID:PMC1914052.</p><p></p>) and achieves maximal induction of approximately 5-fold following three daily doses (3 × 24 hrs) of 100 μg/kg TAM. Significant increases (p < 0.05, n = 5) are denoted by an asterisk (*). In contrast, 100 μg/kg EE (positive control) maximally induced uterine wet weight 11-fold (*, p < 0.05, n = 5) with significant water imbibition (#; p < 0.05, n = 3), while TAM only achieved 50% uterotrophic efficacy and no water imbibition. B) A single dose of 100 μg/kg TAM significantly increased uterine wet weight as early as 24 hrs after administration. No significant water imbibition was observed at any time point

Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice-8

<p><b>Copyright information:</b></p><p>Taken from "Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice"</p><p>BMC Genomics 2007;8():151-151.</p><p>Published online 7 Jun 2007</p><p>PMCID:PMC1914052.</p><p></p>s non-responsive counterpart. These examples further illustrate that the filtering conditions used were adequate to identify differential responses by TAM and EE

Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice-1

<p><b>Copyright information:</b></p><p>Taken from "Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice"</p><p>http://www.biomedcentral.com/1471-2164/8/151</p><p>BMC Genomics 2007;8():151-151.</p><p>Published online 7 Jun 2007</p><p>PMCID:PMC1914052.</p><p></p> EE treatment induced increases in luminal epithelial cell height. Luminal circumference is increased to a greater degree by EE than TAM. Bars represent 20 μm

Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice-2

<p><b>Copyright information:</b></p><p>Taken from "Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice"</p><p>BMC Genomics 2007;8():151-151.</p><p>Published online 7 Jun 2007</p><p>PMCID:PMC1914052.</p><p></p>expression response especially in comparison to EE elicited gene expression [16] is speculated to be due to the delayed absorption of TAM. Inset numbers indicate the number of features represented by each cluster. Black pseudolines indicate the general profile represented within each cluster

Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice-3

<p><b>Copyright information:</b></p><p>Taken from "Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice"</p><p>BMC Genomics 2007;8():151-151.</p><p>Published online 7 Jun 2007</p><p>PMCID:PMC1914052.</p><p></p>poral patterns of expression. Overall, there was good correlation (average ρ = 0.8) between microarray (lines) and QRT-PCR (bars) data. Examples for six of the genes are illustrated. Statistically significant QRT-PCR differences (p < 0.05, n = 4) due to treatment are denoted by an asterisk (*)

Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice-6

<p><b>Copyright information:</b></p><p>Taken from "Comparative temporal and dose-dependent morphological and transcriptional uterine effects elicited by tamoxifen and ethynylestradiol in immature, ovariectomized mice"</p><p>BMC Genomics 2007;8():151-151.</p><p>Published online 7 Jun 2007</p><p>PMCID:PMC1914052.</p><p></p> some cases (e.g., Cdkn1a) a gene classified as Similar may also be classified as EE-Efficacious based on QRT-PCR results due to data compression inherent in microarray data. Statistically significant differences (p < 0.05, n = 4) due to treatment are denoted by an asterisk (*)