3,545 research outputs found
A comparison of blade tip timing data analysis methods
The experimental determination of the vibration characteristics of rotating engine blades is very important for fatigue failure considerations. One of the most promising techniques for measuring the frequency of blade vibrations is blade tip timing. In this paper, three vibration analysis methods were specifically formulated and applied to the tip timing problem for the first time, using data obtained from a simple mathematical blade tip timing simulation. The results from the methods were compared statistically in order to determine which of the techniques is more suitable. One of the methods, the global autoregressive instrumental variables approach, produced satisfactory results at realistic noise levels. However, all of the techniques produced biased results under certain circumstances
A map of the day-night contrast of the extrasolar planet HD 189733b
"Hot Jupiter" extrasolar planets are expected to be tidally locked because
they are close (<0.05 astronomical units, where 1 AU is the average Sun-Earth
distance) to their parent stars, resulting in permanent daysides and
nightsides. By observing systems where the planet and star periodically eclipse
each other, several groups have been able to estimate the temperatures of the
daysides of these planets. A key question is whether the atmosphere is able to
transport the energy incident upon the dayside to the nightside, which will
determine the temperature at different points on the planet's surface. Here we
report observations of HD 189733, the closest of these eclipsing planetary
systems, over half an orbital period, from which we can construct a 'map' of
the distribution of temperatures. We detected the increase in brightness as the
dayside of the planet rotated into view. We estimate a minimum brightness
temperature of 973 +/- 33 K and a maximum brightness temperature of 1212 +/- 11
K at a wavelength of 8 microns, indicating that energy from the irradiated
dayside is efficiently redistributed throughout the atmosphere, in contrast to
a recent claim for another hot Jupiter. Our data indicate that the peak
hemisphere-integrated brightness occurs 166 degrees before opposition,
corresponding to a hot spot shifted east of the substellar point. The secondary
eclipse (when the planet moves behind the star) occurs 120 +/- 24 s later than
predicted, which may indicate a slightly eccentric orbit.Comment: To appear in the May 10 2007 issue of Nature, 10 pages, 2 black and
white figures, 1 colo
The structure of Toho1 β-lactamase in complex with penicillin reveals the role of Tyr105 in substrate recognition
The role of the conserved residue Tyr105 in class A β-lactamases has been the subject of investigation using both structural studies and saturation mutagenesis. Both have shown that while it does not need to be strictly conserved for activity, it is important for substrate recognition. With this in mind we determined the crystal structure of Toho1 β-lactamase at 15 K to 1.10 Å resolution in complex with penicillin. As expected a ring-opened penicillin molecule bound to Ser70 the catalytic nucleophile, can clearly be seen in electron density in the active site. In addition to the trapped penicillin, however, are two additional intact ring-closed penicillin molecules, captured by the enzyme through noncovalent interactions at the edge of the active site
Active-site protonation states in an Acyl-Enzyme intermediate of a Class A β-Lactamase with a Monobactam Substrate
The monobactam antibiotic aztreonam is used to treat cystic fibrosis patients with chronic pulmonary infections colonized by Pseudomonas aeruginosa strains expressing CTX-M extended-spectrum β-lactamases. The protonation states of active-site residues that are responsible for hydrolysis have been determined previously for the apo form of a CTX-M β-lactamase but not for a monobactam acyl-enzyme intermediate. Here we used neutron and high-resolution X-ray crystallography to probe the mechanism by which CTX-M extended-spectrum β-lactamases hydrolyze monobactam antibiotics. In these first reported structures of a class A β-lactamase in an acyl-enzyme complex with aztreonam, we directly observed most of the hydrogen atoms (as deuterium) within the active site. Although Lys 234 is fully protonated in the acyl intermediate, we found that Lys 73 is neutral. These findings are consistent with Lys 73 being able to serve as a general base during the acylation part of the catalytic mechanism, as previously proposed
Particle separation by phase modulated surface acoustic waves
High efficiency isolation of cells or particles from a heterogeneous mixture is a critical processing step in lab-on-a-chip devices. Acoustic techniques offer contactless and label-free manipulation, preserve viability of biological cells, and provide versatility as the applied electrical signal can be adapted to various scenarios. Conventional acoustic separation methods use time-of-flight and achieve separation up to distances of quarter wavelength with limited separation power due to slow gradients in the force. The method proposed here allows separation by half of the wavelength and can be extended by repeating the modulation pattern and can ensure maximum force acting on the particles. In this work, we propose an optimised phase modulation scheme for particle separation in a surface acoustic wave microfluidic device. An expression for the acoustic radiation force arising from the interaction between acoustic waves in the fluid was derived. We demonstrated, for the first time, that the expression of the acoustic radiation force differs in surface acoustic wave and bulk devices, due to the presence of a geometric scaling factor. Two phase modulation schemes are investigated theoretically and experimentally. Theoretical findings were experimentally validated for different mixtures of polystyrene particles confirming that the method offers high selectivity. A Monte-Carlo simulation enabled us to assess performance in real situations, including the effects of particle size variation and non-uniform acoustic field on sorting efficiency and purity, validating the ability to separate particles with high purity and high resolution
Uses of industrial energy benchmarking with reference to the pulp and paper industries
Plant operators and policy makers frequently use energy benchmarking to assess the potential for reducing energy consumption from industrial plants. As benchmarking studies require considerable resources and the cooperation of plant operators it is tempting to try to merge or compare data from different studies. This paper reviews published benchmarks and energy-saving estimates from the paper and pulp industries to explore how comparable data from independent studies are. A literature review was conducted which identified that benchmarks were either produced through a top-down process using annual production and fuel consumption data or through a bottom-up process from process-level data. It was concluded that top-down benchmarks are useful in measuring national trends but are of little value to individual plants. For common process such as Kraft pulp production it is possible to compare values from different studies but only if sufficient information is given in the original studies to confirm that their scope is identical. However, it is unlikely that improvement rates in energy use can be inferred from the difference between studies that use different sources, as the degree of disagreement between contemporary studies is of the same order as the identified potential energy savings. Benchmarking studies were found to provide good summaries of potential technological improvements although there is some inconsistency in estimations of potential impacts.<br/
Swimming using surface acoustic waves
Microactuation of free standing objects in fluids is currently dominated by the rotary propeller, giving rise to a range of potential applications in the military, aeronautic and biomedical fields. Previously, surface acoustic waves (SAWs) have been shown to be of increasing interest in the field of microfluidics, where the refraction of a SAW into a drop of fluid creates a convective flow, a phenomenon generally known as SAW streaming. We now show how SAWs, generated at microelectronic devices, can be used as an efficient method of propulsion actuated by localised fluid streaming. The direction of the force arising from such streaming is optimal when the devices are maintained at the Rayleigh angle. The technique provides propulsion without any moving parts, and, due to the inherent design of the SAW transducer, enables simple control of the direction of travel
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In crystallo-screening for discovery of human norovirus 3C-like protease inhibitors.
Outbreaks of human epidemic nonbacterial gastroenteritis are mainly caused by noroviruses. Viral replication requires a 3C-like cysteine protease (3CLpro) which processes the 200Â kDa viral polyprotein into six functional proteins. The 3CLpro has attracted much interest due to its potential as a target for antiviral drugs. A system for growing high-quality crystals of native Southampton norovirus 3CLpro (SV3CP) has been established, allowing the ligand-free crystal structure to be determined to 1.3Â Ã… in a tetrameric state. This also allowed crystal-based fragment screening to be performed with various compound libraries, ultimately to guide drug discovery for SV3CP. A total of 19 fragments were found to bind to the protease out of the 844 which were screened. Two of the hits were located at the active site of SV3CP and showed good inhibitory activity in kinetic assays. Another 5 were found at the enzyme's putative RNA-binding site and a further 11 were located in the symmetric central cavity of the tetramer
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