Inverse expression of P k and Luke blood group antigens on human RBCs

Luke (LKE) is a high-frequency RBC antigen, related to the P blood group system. A LKE-negative phenotype is found in 1 to 2 percent of donors and may be associated with increased P k . Because P k and similar glycolipids are receptors for shiga toxin on cell membranes, a LKE-negative phenotype could have implications for infections by Shigella dysenteriae and enterohemorrhagic Escherichia coli . STUDY DESIGN AND METHODS: Volunteer donors (n = 257) were serologically typed for LKE with a LKE MoAb, MC813-70. LKE-strong-positive, LKE-weak-positive and LKE-negative RBCs were analyzed for P k , P, LKE, and shiga toxin binding by immunofluorescence flow cytometry, high-performance thin-layer chromatography, scanning densitometry, and high-performance thin-layer chromatography immunostaining. RESULTS: Among Iowa donors, 78.6 percent were LKE-strong-positive, 20.2 percent were LKE-weak-positive, and 1.2 percent were LKE-negative. There was an inverse expression of P k and LKE on RBCs. P k expression was increased on LKE-negative RBCs and was associated with increased shiga toxin binding. A LKE-active glycolipid was identified in the ganglioside fraction of LKE-strong-positive RBCs. CONCLUSION: A LKE-negative phenotype is associated with increased expression of P k on RBCs. Differences in P k and LKE expression may play a role in host susceptibility to infection with S. dysenteriae and E. coli .Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73915/1/j.1537-2995.2001.41070898.x.pd

Performance of Earth Retention System, St. Louis Data Center

The design and performance of the earth retention system for a 35 ft deep excavation in medium clay is described. The earth retention system consisted of soldier piles and lagging with tieback anchors. One level of tiebacks included helical anchors installed in loose to medium dense sand. Behavior of the helical anchors in contrast to conventional drilled-in anchors is discussed

Red blood cell transfusion in palliative care: what are we doing and why are we doing it?

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141052/1/trf14437.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/141052/2/trf14437_am.pd

Drilled Pier Load Test, Fort Collins, Colorado

A full-scale compressive load test was conducted on a drilled pier in the Pierre Shale Formation near Fort Collins, Colorado, to verify design parameters. The test pier was designed based on presumptive design criteria for both end-bearing and skin friction in the shale. The maximum test load of 6.7 MN (750 tons) resulted in a deflection of approximately 230 mm (9.0 in.). Instrumentation within the pier allowed determination of the actual end-bearing and skin friction values at various applied loads. Based on results of the test, production piers were redesigned for skin friction only and shear rings were added to enhance shaft resistance

Soil Property Determination for a Seismic Study

Static and dynamic soil property data were needed for input into the seismic retrofit design for the I-155 Mississippi River crossing located near the center of the New Madrid Seismic Zone. Soils consisted of recent river alluvium underlain by very dense soils of the Mississippi embayment. The field investigation consisted of conventional borings, downhole geophysical tests to measure shear wave velocity, and seismic piezo-cone soundings. SPT energy measurements were made at one boring to confirm hammer energy for liquefaction evaluation. This paper summarizes the data and provides site specific correlation of shear wave velocity vs. N-value from the seismic cone and downhole geophysical tests; measured SPT energy value; and estimates of static and dynamic soil properties

Acquired hemoglobin C secondary to transfusion with antigen‐matched red blood cells

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/107569/1/jca21306.pd

An evaluation of storage time for dithiothreitol‐treated reagent cells

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/138313/1/trf14244.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/138313/2/trf14244_am.pd

Geotechnical Earthquake Engineering for the Great River Bridge

The proposed Great River Bridge is a 1400-foot long cable-stay structure that will be constructed over the Mississippi River between Desha County, Arkansas and Bolivar County, Mississippi. Including the bridge approach structures and approach embankments, the total structure length is approximately 23,500 feet. Seismic issues have controlled most of the structural design. Design ground motions for three typical subsurface profiles were developed and resulted in near-surface peak accelerations between 0.23 and 0.26g. Level ground liquefaction analyses indicated widespread liquefaction in an abandoned channel of the river and sporadic liquefaction elsewhere. Seismic slope stability and lateral spreading analyses indicated minor displacements at the approach embankments, the Arkansas levee, and the Mississippi riverbank; moderate displacements at the Mississippi levee; and major displacements at a 25-foot high natural terrace and the Arkansas riverbank. Conceptual liquefaction mitigation/soil improvement options were investigated

Anti-A and anti-B titers in pooled group O platelets are comparable to apheresis platelets

Although uncommon, acute hemolytic transfusion reactions (AHTRs) have been reported after transfusion of group O single-donor apheresis platelets (SDPs) to group A, B, and AB recipients. Current methods for identifying “high-titer” SDPs include tube and gel methods. The risk of a high-titer unit is considered low with group O, poststorage, pooled platelet concentrates (PPLTs); however, data regarding anti-A and anti-B titers in PPLTs are lacking. STUDY DESIGN AND METHODS: Anti-A and anti-B titers were determined in 185 PPLTs by direct agglutination using manual gel and tube methods. PPLTs tested included 124 group O PPLTs, 25 group A PPLTs, 26 group B PPLTs, and 10 PPLTs containing a mix of either groups O plus A or groups O plus B (mixed PPLTs). The reciprocal of the highest dilution giving macroscopic agglutination was considered the agglutinin titer. RESULTS: Mean anti-A and anti-B titers in group O PPLTs were, respectively, 16 and 8 by tube and 64 and 32 by gel (p < 0.0001). Gel titers were one to two dilutions higher than tube and sensitive to reagent red cell lots. With the use of at least 64 as a critical titer, 60 percent of group O PPLTs tested by gel would be considered high-titer. In mixed PPLTs, the addition of one non-group O PLT significantly decreased or neutralized the corresponding anti-A or anti-B (p < 0.0001). CONCLUSION: Anti-A and anti-B titers in group O PPLTs are comparable to those reported in group O SDPs and significantly lower than titers reported in AHTR. A critical direct agglutinin titer of 64 for identifying high-titer units by gel is too low and should be increased to 128 or higher.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73545/1/j.1537-2995.2008.01814.x.pd

How do I perform whole blood exchange?

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154397/1/trf15660_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154397/2/trf15660.pd