A new method for automatic Multiple Partial Discharge Classification

A new wavelet based feature parameter have been developed to represent the characteristics of PD activities, i.e. the wavelet decomposition energy of PD pulses measured from non-conventional ultra wide bandwidth PD sensors such as capacitive couplers (CC) or high frequency current transformers (HFCT). The generated feature vectors can contain different dimensions depending on the length of recorded pulses. These high dimensional feature vectors can then be processed using Principal Component Analysis (PCA) to map the data into a three dimensional space whilst the first three most significant components representing the feature vector are preserved. In the three dimensional mapped space, an automatic Density-Based Spatial Clustering of Applications with Noise (DBSCAN) algorithm is then applied to classify the data cluster(s) produced by the PCA. As the procedure is undertaken in a three dimensional space, the obtained clustering results can be easily assessed. The classified PD sub-data sets are then reconstructed in the time domain as phase-resolved patterns to facilitate PD source type identification. The proposed approach has been successfully applied to PD data measured from electrical machines and power cables where measurements were undertaken in different laboratories

Copper Bioavailability and Leaching in Conventional and Organic Viticulture under Environmental Stress

The continuous use of Cu-based fungicides in viticulture has caused Cu accumulation in soils, which represents a major environmental and toxicological concern. The purpose of this study was to verify whether the organic management would be more resilient to temperature and moisture stresses in comparison to conventional practices. Two organic and two conventional vineyard soils, contrasting in pH, were exposed for six months to temperature stress (29 °C), moisture stress (10% water holding capacity—WHC), and combined stresses (29 °C—10% WHC). Main soil properties, Cu speciation, bioavailability, and leaching were monitored before and after 6 months’ incubation. Results confirm that the increase of temperature caused a decrease in both total organic carbon (TOC) and dissolved organic carbon (DOC) (up to 19% and 49%, respectively), a decrease in available ammonium, and an increase in nitrate. Moisture stress tends to mitigate some of these changes. Despite that, changes of Cu bioavailability and leaching were limited and rarely significant. Moreover, no regular trends between conventional and organic management could be observed. Changes in soil pH and total N (TN) appeared as the most influencing properties to regulate Cu behavior in vineyard soils. Calcareous soils were more resistant to environmental stresses compared to acid soils, regardless of the type of management (conventional or organic)

Development of quartz fiber calorimetry

Embedding radiation hard (up to Gigarad levels) silica optical fibres into an absorber is proposed for applications in very forward LHC calorimeters. The shower particles produce Cherenkov light. The main advantages of this solution are the fast response (signal duration below 10 ns), transverse dimension of the visible energy of hadronic showers of the order of 1 cm, and insensitivity to radio- and neutron- activation. A comprehensive study of the performance of such detector is proposed

Temperature changes and the ATP concentration of the soil microbial biomass

Two soils from temperate sites (UK; arable and grassland) were incubated aerobically at 0, 5, 15 or 258C for up to 23 days. During this period both soils were analysed for soil microbial biomass carbon (biomass C) and adenosine 5' triphosphate contents (ATP). Biomass C did not change signi\uaecantly in either soil at any temperature throughout, except during days 0 to 1 in the grassland soil. Soil ATP contents increased slowly throughout the 23 days of incubation, from 2.2 to a maximum of 3.1 nmol ATP g \uff1 soil in the arable soil (a 40% increase) and from 6.2 to a maximum of 11.2 nmol ATP g \uff1 soil in the grassland soil (an increase of 81%), both at 258C. Since biomass C did not change either with increasing temperature or increasing time of incubation, it was concluded that an increase in ATP was either due to an increase in adenylate energy charge or de novo synthesis of ATP, or both. During the incubation, biomass ATP concentrations ranged from about 5 to 12 mmol ATP g \uff1 biomass C but trends between biomass ATP and incubation temperatures were not very obvious until about day 13. On day 23, biomass ATP concentrations were positively and linearly related to temperature: (mmol ATP g \uff1 biomass C = 6.9820.35 + 0.13420.023 T0 (r 2 = 0.77) with no signi\uaecant di erence in the slope between the grassland and arable soils. At 258C the biomass ATP concentration was 10.3 mmol g \uff1 biomass C, remarkably close to many other published values. It was concluded that, although the biomass increased its ATP concentration in response to increasing temperature, the increase was comparatively small. Also, at all temperatures tested, the biomass maintained its ATP concentration within the range commonly reported for micro-organisms growing expontentially in vitro. This is despite the fact that the biomass normally exhibits other features more typical of a ``resting'' or dormant population 0 a paradox which still is not resolved

Organic vs. conventional: impact of cultivation treatments on the soil microbiota in the vineyard

The aim of this study was to compare the effects of two vineyard management practices on the soil and its associated microbiota. The experiments were conducted in two adjacent plots, one completely organically managed and the other conventionally managed in terms of phytosanitary treatments but fertilized with organic amendments. The chemical soil analyses were correlated to the prokaryotic and fungal communities, which were studied using the metabarcoding technique. The main difference between the two treatments was a significantly higher amount of Cu in the organic managed vineyard soil, while conventional managed soil presented higher concentration of Na and Mg and was also associated with higher pH values. Despite these differences, no significant diversities were observed on soil biodiversity and microbial composition considering alpha and beta diversity metrics. However, the percentages of some phyla analyzed individually differed significantly between the two managements. Analyzing the metabolisms of these phyla, it was discovered an increment of species correlated to soils with higher organic matter content or land not used for agricultural purposes in the organic treated soil. The findings indicate that, despite the use of copper-based phytosanitary products, there was no degradation and loss of biodiversity in the organic soil microbial population compared to conventional management with the same type of fertilization, and the observed microbial population was more similar to that of natural soils

Flooding by sea and brackish waters enhances mobility of Cd, Zn and Pb from airborne dusts in coastal soils

Sea level rise and extreme weather conditions caused by climatic changes enhance the frequency and length of submersion events in coastal soils, causing deposited airborne dusts to get in contact with marine salts. The behaviour of Cd, Zn and Pb from pedogenetic minerals and from dusts from mining and smelting activities, added to two soils under different agricultural management (arable and grassland) was examined after soil flooding for 1, 7 and 30 days with waters of increasing salinities (0, 4.37, 8.75, 17.25 and 34.5 g L−1). A rain water event following 1 d flooding released an extra amount of metals. Concentration of potentially toxic elements (PTE), pH, dissolved inorganic and organic C were measured in solutions collected by gravity from soil columns. Speciation distribution of leached metals and oversaturation parameters were calculated by Visual Minteq 3.0 and showed that complexation by chloride ions for Cd and fulvic acids for Pb were the drivers of solubilisation, while Zn interacted with both. Results showed that marine salts enhance up to 300 times leaching of Cd, and several times that of Zn and Pb from contaminated soils and that airborne toxic elements are much more mobilized than pedogenic ones. Smelter exhaust metals, particularly Pb, were made more mobile than those in mine tailings (up to 55 against 0.7 ng μg-1 Pb). Soil management strongly also influence mobilization by saline water: much lower amounts were leached from the grassland soil. Soil organic matter quality (DOC and humification) affects the extent of mobilization. The length of the flooding period did not result in coherent time trend patterns for the three metals, probably because of the multiple changes in solution parameters, but leached metals were always highly linearly correlated negatively with pH and positively with DOC

A rapid and simple uhplc-ms/ms method for quantification of plasma globotriaosylsphingosine (Lyso-gb3)

Fabry disease (FD) is a rare X-linked lysosomal storage disorder caused by α-galactosidase A gene (GLA) mutations, resulting in loss of activity of the lysosomal hydrolase, α-galactosidase A (α-Gal A). As a result, the main glycosphingolipid substrates, globotriaosylceramide (Gb3) and globotriaosylsphingosine (lyso-Gb3), accumulate in plasma, urine, and tissues. Here, we propose a simple, fast, and sensitive method for plasma quantification of lyso-Gb3, the most promising secondary screening target for FD. Assisted protein precipitation with methanol using Phree cartridges was performed as sample pre-treatment and plasma concentrations were measured using UHPLC-MS/MS operating in MRM positive electrospray ionization. Method validation provided ex-cellent results for the whole calibration range (0.25–100 ng/mL). Intra-assay and inter-assay accuracy and precision (CV%) were calculated as <10%. The method was successfully applied to 55 plasma samples obtained from 34 patients with FD, 5 individuals carrying non-relevant polymorphisms of the GLA gene, and 16 healthy controls. Plasma lyso-Gb3 concentrations were larger in both male and female FD groups compared to healthy subjects (p < 0.001). Normal levels of plasma lyso-Gb3 were observed for patients carrying non-relevant mutations of the GLA gene compared to the control group (p = 0.141). Dropping the lower limit of quantification (LLOQ) to 0.25 ng/mL allowed us to set the optimal plasma lyso-Gb3 cut-off value between FD patients and healthy controls at 0.6 ng/mL, with a sensitivity of 97.1%, specificity of 100%, and accuracy of 0.998 expressed by the area under the ROC curve (C.I. 0.992 to 1.000, p-value < 0.001). Based on the results obtained, this method can be a reliable tool for early phenotypic assignment, assessing diagnoses in patients with borderline GalA activity, and confirming non-relevant mutations of the GLA gene

Ultrastructural and spectrophotometric study on the effects of putative triggers on aortic valve interstitial cells in in vitro models simulating metastatic calcification.

Metastatic calcification of cardiac valves is a common complication in patients affected by chronic renal failure. In this study, primary bovine aortic valve interstitial cells (AVICs) were subjected to pro-calcific treatments consisting in cell stimulation with (i) elevated inorganic phosphate (Pi = 3mM), in order to simulate hyperphosphatemic conditions; (ii) bacterial endotoxin lipopolysaccharide (LPS), simulating direct effects by microbial agents; and (iii) conditioned media (CM) derived from cultures of either LPS-stimulated heterogenic macrophages (commercial murine RAW264.7 cells) or LPS-stimulated fresh allogeneic monocytes/macrophages (bCM), simulating consequent inflammatory responses, alone or combined. Compared to control cultures, spectrophotometric assays revealed shared treatment-dependent higher values of both calcium amounts and alkaline phosphatase activity for cultures involving the presence of elevated Pi. Ultrastructurally, shared peculiar pro-calcific degeneration patterns were exhibited by AVICs from the same cultures irrespectively of the applied treatment. Disappearance of all cytomembranes and concurrent formation of material showing positivity to Cuprolinic Blue and co-localizing with silver precipitation were followed by the outcropping of such a material, which transformed in layers outlining the dead cells. Subsequent budding of these layers resulted in the formation of bubbling bodies and concentrically laminated calcospherulae mirroring those in actual soft tissue calcification. In conclusion, the in vitro models employed appear to be reliable tools for simulating metastatic calcification and indicate that hyperphosphatemic-like conditions could trigger valve calcification per se, with LPS and allogeneic macrophage-derived secretory products acting as possible calcific enhancers via inflammatory responses

Bioaccumulation of polycyclic aromatic hydrocarbons and survival of earthworms (Eisenia andrei) exposed to biochar amended soils

Biochar has a charcoal polycyclic aromatic structure which allows its long half-life in soil, making it an ideal tool for C sequestration and for adsorption of organic pollutants, but at the same time raises concerns about possible adverse impacts on soil biota. Two biochars were tested under laboratory-controlled conditions on Eisenia andrei earthworms: a biochar produced at low temperature from wine tree cuttings (WTB) and a commercial low tar hardwood lump charcoal (HLB). The avoidance test (48-h exposure) showed that earthworms avoid biochar-treated soil with rates higher than 16 t ha 121 for HLB and 64 t ha 121 for WTB. After 42 days, toxic effects on earthworms were observed even at application rates (100 t ha 121) that are generally considered beneficial for most crops. The concentration of HLB and WTB required to kill half of earthworms\u2019 population (LC50; 95 % confidence limits) in the synthetic OECD soil was 338 and 580 t ha 121, respectively. Accumulation of polycyclic aromatic hydrocarbons (PAH) in earthworms exposed to the two biochar types at 100 t ha 121 was tested in two soils of different texture. In biochar-treated soils, the average earthworm survival rates were about 64 % in the sandy and 78 % clay-loam soils. PAH accumulation was larger in the sandy soil and largest in soils amended with HLB. PAH with less than four rings were preferentially scavenged from the soil by biochars, and this behaviour may mask that of the more dangerous components (i.e. four to five rings), which are preferentially accumulated. Earthworms can accumulate PAH as a consequence of exposure to biochar-treated soils and transfer them along the food chain. Soil type and biochar quality are both relevant in determining PAH transfer