Additional file 1: Table S1. of Two-year post-discharge costs of care among patients treated with transcatheter or surgical aortic valve replacement in Germany

Marginal effects for the two parts of the two-part model separately. Marginal effects are shown for the two parts of the two-part model separately. As shown in Table 2, two-part models with one (time: month 1 vs. month 2–24, or month 1–12 vs month 13–24) or two (time and sex or procedure or ...) categorical covariates are conducted with a logistic regression analysis for part one and a generalised linear model with the log link and gamma distribution for the second part. Marginal effects for the combined models are shown on the raw scale (€ per month). 95% confidence intervals in brackets. All estimated prices reflect cost estimates in Euro (basis year 2011) from a societal perspective. (DOCX 38 kb

Additional file 2: Table S2. of Two-year post-discharge costs of care among patients treated with transcatheter or surgical aortic valve replacement in Germany

Two year monthly cost estimates of patients treated for aortic valve stenosis (including also patients receiving drug-based therapy). Separate two-part models, with one (only time: month 1 vs. month 2–24, or month 1–12 vs month 13–24) or two (time and sex or procedure or ...) categorical covariates are conducted with a logistic regression analysis for part one and a generalised linear model with the log link and gamma distribution for the second part. Marginal effects for the combined models are shown on the raw scale (€ per month). 95% confidence intervals in brackets. All estimates reflect cost estimates in Euro (basis year 2011) from a societal perspective. (DOCX 25 kb

TRAF6 expression in blood does not associate with acute or chronic coronary heart disease.

<p>325 patients undergoing coronary angiography were divided into three groups: no coronary heart disease (No CHD, N = 77), stable coronary heart disease (CHD, N = 178), and acute coronary syndromes (ACS, N = 70). TRAF6 and GAPDH mRNA was analyzed by quantitative real-time PCR in total blood RNA. Results are presented as mean±SD computed from the average measurements obtained from each group.</p

TRAF6 deficiency does not alter lipid deposition in the abdominal aorta.

<p>Lethally irradiated 6 week old TRAF6^+/+/LDLR^−/− mice received TRAF6-deficient (hatched bars, N = 10) or competent fetal liver cells (white bars, N = 10), TRAF6^+/−/LDLR^−/− mice received TRAF6-deficient fetal liver cells (black bars, N = 10) only. Subsequently, all groups consumed high cholesterol diet (HCD) for 18 weeks. Abdominal aortas were fixed in formalin, pinned, and stained with oil red O to detect lipid deposition. Oil red O-positive staining in per cent of total area is shown as mean±SEM in the upper panel (A), representative images are shown below (B).</p

TRAF6 deficiency does not modulate atherogenesis in mice.

<p>Lethally irradiated 6 week old TRAF6^+/+/LDLR^−/− mice received TRAF6-deficient (hatched bars, N = 21) or competent fetal liver cells (white bars, N = 21), TRAF6^+/−/LDLR^−/− mice received TRAF6-deficient fetal liver cells (black bars, N = 22) only. Subsequently, all groups consumed high cholesterol diet (HCD) for 18 weeks. Intimal lesion area of the atherosclerotic plaques in aortic roots was quantified. Pooled mean intimal lesion area ± SEM are shown as graphs in the upper panel (A), representative sections stained with oil red O below (B).</p

Subtypes of blood leukocytes.

<p>Subtypes of blood leukocytes.</p

TRAF6 deficiency does not modulate inflammatory reactivity of macrophages toward cholesterol and palmitic acid.

<p>Bone marrow-derived macrophages were isolated from 6 week old TRAF6^+/+/LDLR^−/− mice receiving TRAF6-deficient (hatched bars, N = 4) or competent fetal liver cells (white bars, N = 4), were stimulated with 4 mg/ml cholesterol or 0.75 µM palmitic acid, and assayed for expression of IL-6 (A), MCP-1 (B), TNFα (C), and IL12-p70 (D) by cytometric bead array.</p

Successful reconstitution of peripheral blood cells by fetal liver cell transplantation.

<p>LDLR^−/− mice (CD45.2-positive/CD45.1-negative, A) were lethally irradiated (2×450 cGy) and reconstituted with fetal liver cells of 6–8 week old CD45.1-positive/CD45.2-negative mice (B). After an interval of 4 weeks, peripheral blood cells were immunostained with CD45.1-PE and CD45.2-FITC (exemplary donors are shown in C and D) or CD45.1-PE in combination with CD19-PECy (B-cell marker, E), CD3-APC (T-cell marker, F), and CD11b-FITC (monocytic marker, G) antibodies and analyzed by FACS.</p

Demographic and clinical characteristics of study participants.

<p>*, p<0.05 vs controls.</p><p>∫, p<0.05 vs CHD.</p

Weights, cholesterol-, and leukocyte levels before and after high cholesterol diet.

<p>Lethally irradiated 6 week old TRAF6^+/+/LDLR^−/− mice received TRAF6-deficient (hatched bars, N = 22) or competent fetal liver cells (white bars, N = 27), TRAF6^+/−/LDLR^−/− mice received TRAF6-deficient fetal liver cells (black bar, N = 23) only. Subsequently, all groups consumed high cholesterol diet (HCD) for 18 weeks. Weights (A), plasma cholesterol levels (B), triglycerides (C), and leukocyte counts (D) were taken before and after HCD.</p