Interdependence of productive effort and in vitro vegetal extract treatment on specific cell-mediated immunity in horses

The research aimed at identifying and rendering alcoholic extracts of various plants based on their in vitro potential in stimulating cell mediated immunity and in alleviating stress effects subsequent to workout type in horses. Experimental horses were selected from different working environments: (a) draft, agricultural works-n = 16, average age 8 years, (b) inconstant effort, leisure, n = 15, average age 7.5 years and (c) constant training, endurance, average age 3.5 years. Blood samples were collected in heparinized vials (50 UI/ml) before and after the workout. Alcoholic extracts (2 mu l/well, duplicate) of Taraxacum officinale (L) Weber, Symphytum officinale L., Equisetum palustre L., Viola tricolor L., Avena sativa L., Capsella bursa pastoris (L) Medik., Hypericum perforatum L., Chelidonium majus L. were investigated by blast transformation test and statistically interpreted (Students t test). In vitro responses were the highest to all tested extracts in endurance horses and the lowest in draft horses (p<0.01-0.001). C. bursa pastoris. was the most efficient in draft and endurance categories before the workout (draft 45.13 +/- 8.05, in leisure 48.09 +/- 24.13 and 53.44 +/- 9.32 in endurance horses), but not after the workout, where C. majus performed better (41.86 +/- 22.47 in leisure and 48.1 +/- 6.82 in endurance horses), probably due to its complex, protein-rich latex structure. The effects of plant extracts depended on the type of effort and the level of constant training rather than on the taxonomy of the plant

The Dynamics of Starch and Total Sugars during Fruit Development for Ionathan, Starkrimson and Golden Delicious Apple Varieties

Apples represent one of the most important elements in the human diet. The research carried out involved three apple varieties (Starkimson, Ionathan and Golden Delicious) harvested during development, which were analyzed in terms of physico-chemical profile. During fruit development, diameter and mass increased continuously for all the three apple varieties studied, acidity decreased and total soluble solids content increased significantly. The pH values of the apple pulp also increased confirming the results obtained for the acidity. The starch concentration was reduced in the early stages of fruit development in all three varieties of apple studied. Starch concentration significantly increased (p <0.05) starting 35 days after full bloom, similar for all three varieties studied. The peak of starch concentration for all three varieties under study was reached at 65 days after full bloom. Starch concentration gradually decreased starting 107 days after full bloom until technological maturity. The results provide important information on how to make the best use of the apple cultivars investigated, and not only, for both technological research and processing practice. Also, they determine the best time to use the apples from physiological falls in different foods and pharmaceuticals

Skin Bacteriome and its Resistance to Antibiotics in Free Range Pigs

The cutaneous microbiome and also its resistance to antibiotics is exposed to change, depending on different habitat factors. This research investigates the composition of cutaneous microflora and its antibiotic resistance in pigs raised on free range farms (mix breed swine, from low input small farms). Swabs were collected from the skin surface and subjected to classical microbiological methods (simple broth and nutrient agar cultivation, colony isolation and biochemical API identification). The antibacterial resistance to gentamicin, streptomycin, oxitetracycline, tylosin, amoxacillin-clavulanic acid, marbofloxacin, tulatromycin, cefotaxime and doxycycline was estimated by Kirby Bauer method and multiple antibiotic resistance (MAR) index was calculated. Strains from Staphylococcus (sciuri and warnerii), Shigella spp., Kytococcus sedentarius, Salmonella spp. and Citrobacter freundii genera and species were identified in the collected samples. The most resistant was a S. warnerii strain, but the MAR index was high (0.33) in 50% of the strains. The most efficient antibiotic was cefotaxime and the least efficient was oxitetracycline. The results indicated the presence of antibiotic resistant ubiquitous and pathogenic strains in the investigated pigs which need caution, since they could express pathogenicity under appropriate conditions which low input farming system could provide

Bactericidal Capacity of Serum and Enhancement of Specific Cell-Mediated Immunity Subsequent to Hipopphae Rhamnoides Treatment in Pigs

Low-input farming subject’s swine to increased immune stress and subsequent infectious risk, which could be prevented by phytotherapy. Hypopphae rhamnoides is a widespread shrub in both Europe and Asia. The research aimed at investigating the influence of a whole fruit extract on antibacterial effect of the serum in extensively raised, two batches, sea buckthorn in vivo treated/untreated pigs. Scavenging effect over DPPH was used to estimate the antioxidant potential of the sea buckthorn commercial syrup (Steaua Divina©). Minimal inhibiting (MIC) and minimal bactericidal (MBC) capacity of the serum were tested and spectrophotometrically measured against Shigella spp., Kitococcus sedentarius, Staphylococcus werneri and Staphylococcus sciuri. The antioxidant activity of the syrup was intermediate (54.65 %). Sera from treated pigs had bactericidal and bacteriostatic effect on Shigella spp. and S. sciuri, respectively. Shigella spp. growth was inhibited at serum dilutions of ½-1/32 while much lower ½-1/4 dilutions were active against S. sciuri. In untreated pigs, the sera were inefficient in either stopping or totally inhibiting bacterial growth. The MBC and MIC values obtained for the sera of pigs treated with Hippophae rhamnoides syrup supported the positive effect of the in vivo treatment inducive of potential protection against bacterial diseases

Fate of Biodegradable Engineered Nanoparticles Used in Veterinary Medicine as Delivery Systems from a One Health Perspective

The field of veterinary medicine needs new solutions to address the current challenges of antibiotic resistance and the need for increased animal production. In response, a multitude of delivery systems have been developed in the last 20 years in the form of engineered nanoparticles (ENPs), a subclass of which are polymeric, biodegradable ENPs, that are biocompatible and biodegradable (pbENPs). These platforms have been developed to deliver cargo, such as antibiotics, vaccines, and hormones, and in general, have been shown to be beneficial in many regards, particularly when comparing the efficacy of the delivered drugs to that of the conventional drug applications. However, the fate of pbENPs developed for veterinary applications is poorly understood. pbENPs undergo biotransformation as they are transferred from one ecosystem to another, and these transformations greatly affect their impact on health and the environment. This review addresses nanoparticle fate and impact on animals, the environment, and humans from a One Health perspective

M-Mode Echocardiography in Canine Veterinary Practice: A Comprehensive Review of Left Ventricular Measurements in 44 Different Dog Breeds

This review article focuses on the use of canine M-mode in veterinary medicine, specifically in assessing the left ventricle measurements in several breeds. It traces the historical development of echocardiography techniques, including A-mode, B-mode, and motion mode (M-mode), which provide accurate unidimensional records of cardiac structures. This article highlights the significance of M-mode measurements in diagnosing stage B2 of MMVD, where left ventricular end-diastolic internal diameter corrected with body weight (LVIDdN) is essential for identifying cardiac enlargement. It also explains the role of M-mode in diagnosing DCM, outlining criteria such as left ventricular dilatation. The authors emphasize the importance of breed-specific reference values for echocardiographic measurements due to variations in somatotype among dogs. This review provides a comprehensive table summarizing M-mode measurements of the left ventricle for 44 different dog breeds, including interventricular septum thickness, left ventricular internal diameter, and left ventricular posterior wall thickness during systole and diastole. This review’s methodology involves compiling data from various scientific literature sources, providing an extensive tabular representation of M-mode measurements for different breeds, ages, and sexes. Overall, this review highlights the critical role of M-mode echocardiography in diagnosing and managing cardiac diseases in dogs, underscores the importance of breed-specific reference values, and presents a comprehensive summary of M-mode measurements for various dog breeds, aiding both clinicians and researchers

Enhanced responses in antigen primed dogs after administration of a complex microbial immune stimulating compound

The research aimed to investigate the efficacy of antiparvoviral monovalent vaccine for puppies treated with a complex poly-bacterial compound. The experiment was carried out on 17 Labrador Retriever puppies, from 41 to 63 days of age. The two groups (experimental and control) were subjected to: a) a polybacterial preparation and antiparvoviral vaccine (n = 12) and b) to antiparvoviral vaccine (n = 5), respectively. One ml of a poly-bacterial preparation was administered on days 0, 2, 4, 6, while the antiparvoviral vaccine Duramune KF11 was injected on day 8 of the experiment. Phagocytic activity and total lysozyme concentrations were measured on days 0, 8 and 22 of the experiment. The global immunostimulating effects of the poly-bacterial preparation on the innate immune system of vaccinated puppies increased when compared to those of the control variant

Fate of Biodegradable Engineered Nanoparticles Used in Veterinary Medicine as Delivery Systems from a One Health Perspective

The field of veterinary medicine needs new solutions to address the current challenges of antibiotic resistance and the need for increased animal production. In response, a multitude of delivery systems have been developed in the last 20 years in the form of engineered nanoparticles (ENPs), a subclass of which are polymeric, biodegradable ENPs, that are biocompatible and biodegradable (pbENPs). These platforms have been developed to deliver cargo, such as antibiotics, vaccines, and hormones, and in general, have been shown to be beneficial in many regards, particularly when comparing the efficacy of the delivered drugs to that of the conventional drug applications. However, the fate of pbENPs developed for veterinary applications is poorly understood. pbENPs undergo biotransformation as they are transferred from one ecosystem to another, and these transformations greatly affect their impact on health and the environment. This review addresses nanoparticle fate and impact on animals, the environment, and humans from a One Health perspective