Human induced pluripotent stem cell-derived planar neural organoids assembled on synthetic hydrogels

The tailorable properties of synthetic polyethylene glycol (PEG) hydrogels make them an attractive substrate for human organoid assembly. Here, we formed human neural organoids from iPSC-derived progenitor cells in two distinct formats: (i) cells seeded on a Matrigel surface; and (ii) cells seeded on a synthetic PEG hydrogel surface. Tissue assembly on synthetic PEG hydrogels resulted in three dimensional (3D) planar neural organoids with greater neuronal diversity, greater expression of neurovascular and neuroinflammatory genes, and reduced variability when compared with tissues assembled upon Matrigel. Further, our 3D human tissue assembly approach occurred in an open cell culture format and created a tissue that was sufficiently translucent to allow for continuous imaging. Planar neural organoids formed on PEG hydrogels also showed higher expression of neural, vascular, and neuroinflammatory genes when compared to traditional brain organoids grown in Matrigel suspensions. Further, planar neural organoids contained functional microglia that responded to pro-inflammatory stimuli, and were responsive to anti-inflammatory drugs. These results demonstrate that the PEG hydrogel neural organoids can be used as a physiologically relevant in vitro model of neuro-inflammation

sj-docx-1-tej-10.1177_20417314241230633 – Supplemental material for Human induced pluripotent stem cell-derived planar neural organoids assembled on synthetic hydrogels

Supplemental material, sj-docx-1-tej-10.1177_20417314241230633 for Human induced pluripotent stem cell-derived planar neural organoids assembled on synthetic hydrogels by Joydeb Majumder, Elizabeth E Torr, Elizabeth A Aisenbrey, Connie S Lebakken, Peter F Favreau, William D Richards, Yanhong Yin, Qiang Chang and William L Murphy in Journal of Tissue Engineering</p

Figure 6

Material-dependent maintenance of hESC pluripotenc

Supplemental Figure 12

Shear modulus of synthetic hydrogels used in hESC cultur