Circadian Organization and Microbiology: Variance Spectra and a Periodogram on Behavior of Escherichia coli Growing in Fluid Culture

Over three decades ago Rogers and Greenbank (1) published observations on intermittent growth in a bacterial culture. These interesting data are analyzed further in this report by computational procedures ( 2-8) designed for detecting and evaluating the significance of nearly periodic phenomena. By such methods, circadian (about 24-hour) periodicity in a culture of E. coli is unmasked as a significant component of the recorded changes. These results extend the already broad scope of circadian temporal organization in microbiology

Deformation and crystallization of Zr-based amorphous alloys in homogeneous flow regime

The purpose of this study is to experimentally investigate the interaction of inelastic deformation and microstructural changes of two Zr-based bulk metallic glasses (BMGs): Zr_(41.25)Ti_(13.75)Cu_(12.5)Ni_(10)Be_(22.5) (commercially designated as Vitreloy 1 or Vit1) and Zr_(46.75)Ti_(8.25)Cu_(7.5)Ni_(10)Be_(27.5) (Vitreloy 4, Vit4). High-temperature uniaxial compression tests were performed on the two Zr alloys at various strain rates, followed by structural characterization using differential scanning calorimetry (DSC) and transmission electron microscopy (TEM). Two distinct modes of mechanically induced atomic disordering in the two alloys were observed, with Vit1 featuring clear phase separation and crystallization after deformation as observed with TEM, while Vit4 showing only structural relaxation with no crystallization. The influence of the structural changes on the mechanical behaviors of the two materials was further investigated by jump-in-strain-rate tests, and flow softening was observed in Vit4. A free volume theory was applied to explain the deformation behaviors, and the activation volumes were calculated for both alloys

Adult bobcat (\u3ci\u3eLynx rufus\u3c/i\u3e) habitat selection in a longleaf pine savanna

Background: Pine savannas are primarily managed with frequent prescribed fire (≤ 3 years) to promote diversity of flora and fauna, and to maintain open, park-like conditions needed by species such as the endangered red-cockaded woodpecker (Picoides borealis). However, a knowledge gap exists in our understanding of bobcat (Lynx rufus) habitat selection in longleaf pine savannas and research is warranted to direct our future management decisions. Methods: We examined bobcat habitat selection in a pine savanna managed with frequent fires at two spatial scales (i.e., study area boundary [hereafter, landscape scale]) and annual area of use [95% kernel density; local scale]), and assessed effects of prescribed fire on bobcat habitat selection. Specifically, we monitored 45 bobcats (16 males and 29 females) during 2001–2007. Results: We found differential habitat selection by sex. At the landscape scale, female bobcats were closer to mixed pine-hardwoods, young pine, and secondary roads, but farther from mature pine and hardwoods stands relative to males. We found no difference in selection of agriculture, shrub-scrub, and primary roads between sexes. At the annual area of use scale, female bobcats were closer to secondary roads, but farther from agriculture and shrub-scrub relative to males. We found no difference in selection of mature pine, mixed pine-hardwoods, hardwoods, young pine, and primary roads between sexes. Bobcats primarily selected for stands burned ≤ 1.1 years post-fire. Conclusions: Our results show that bobcats exploit a broad range of habitat types in pine landscapes managed with frequent fire and commonly use recently burned stands (≤ 1.1 year post-fire), suggesting prey in many areas of this system are at risk of bobcat predation. Additionally, we suggest land managers consider scale of selection by bobcats when developing habitat management strategies

Eastern wild turkey nest site selection in two frequently burned pine savannas

Introduction: Reproductive success is a critical factor affecting avian demographics and can be influenced by many factors including nesting chronology, predation risk, and fine-scale nest site selection. Methods: We modeled the relative influences of habitat-related covariates at six spatial scales (nest site: 15-, 40-, 80-, 120-, 160-, and 200-m radii) on Eastern wild turkey (Meleagris gallopavo silvestris) nest site selection in two pine savannas managed by frequent prescribed fire (≤3 years) in southwestern Georgia during 2011–2013. Results: Nest site (15-m scale) habitat metrics (mean visual obstruction [cm] and canopy closure [%]) had the greatest influence on nest site selection relative to covariates measured at larger spatial scales. Scaled odds ratios suggested that nests were 26.8 % more likely to occur for every 10 cm increase in mean vegetation height with a range of 7.5 to 150.0 cm and 18.5 % less likely to occur for every 10 % increase in canopy closure with a range from 0.0 to 97.8 %. Total ground cover, canopy closure, edge density, and percent land cover type (e.g., mature pine, mixed pine/hardwood, shrub/scrub) had minimal influence on nest site selection. Conclusions: Management of pine savannas for turkey nest sites should focus on creating early-successional vegetation to conceal nests from potential predators. Additionally, we suggest that future studies consider evaluating the influence of spatial scale on turkey nest site selection

Survival and cause-specific mortality of female eastern wild turkeys in two frequently-burned longleaf pine savannas

Longleaf pine savannas have declined throughout the southeastern United States due to land-use change. Fortunately, natural resource professionals are currently restoring these ecologically and economically important savannas. Although efforts are underway to restore longleaf pine savannas, little information exists on female eastern wild turkey Meleagris gallopavo silvestris population dynamics in these systems. Therefore, we evaluated survival and cause-specific mortality of female eastern wild turkeys in two longleaf pine savannas in southwestern Georgia. We radio-marked 126 female wild turkeys during 2010–2013 and monitored their survival; 66 (52.4%) radio-marked females died during the study. We estimated causes of death for 37 mortality events with predation serving as the leading known cause of mortality, with 35.1% of mortalities attributed to mesocarnivore predation (e.g., bobcat Lynx rufus, coyote Canis latrans, and gray fox Urocyon cinereoargenteus) and 18.9% to great-horned owl Bubo virginianus predation. One female (2.7%) was hit by a vehicle. Seasonal survival estimates varied from a high during fall (Ŝ = 0.94; 95% CI: 0.86–1.00) to a low during spring (Ŝ = 0.76; 95% CI: 0.68–0.87). Survival of incubating females was 0.82 (95% CI: 0.71–0.93) and survival of nonincubating females was 0.67 (95% CI: 0.52–0.87). Annual survival was 0.55 (95% CI: 0.44–0.67). To ensure sustainable wild turkey populations in longleaf pine savannas, we suggest managers monitor relationships between survival and population productivity

Survival and cause-specific mortality of female eastern wild turkeys in two frequently-burned longleaf pine savannas

Longleaf pine savannas have declined throughout the southeastern United States due to land-use change. Fortunately, natural resource professionals are currently restoring these ecologically and economically important savannas. Although efforts are underway to restore longleaf pine savannas, little information exists on female eastern wild turkey Meleagris gallopavo silvestris population dynamics in these systems. Therefore, we evaluated survival and cause-specific mortality of female eastern wild turkeys in two longleaf pine savannas in southwestern Georgia. We radio-marked 126 female wild turkeys during 2010–2013 and monitored their survival; 66 (52.4%) radio-marked females died during the study. We estimated causes of death for 37 mortality events with predation serving as the leading known cause of mortality, with 35.1% of mortalities attributed to mesocarnivore predation (e.g., bobcat Lynx rufus, coyote Canis latrans, and gray fox Urocyon cinereoargenteus) and 18.9% to great-horned owl Bubo virginianus predation. One female (2.7%) was hit by a vehicle. Seasonal survival estimates varied from a high during fall (Ŝ = 0.94; 95% CI: 0.86–1.00) to a low during spring (Ŝ = 0.76; 95% CI: 0.68–0.87). Survival of incubating females was 0.82 (95% CI: 0.71–0.93) and survival of nonincubating females was 0.67 (95% CI: 0.52–0.87). Annual survival was 0.55 (95% CI: 0.44–0.67). To ensure sustainable wild turkey populations in longleaf pine savannas, we suggest managers monitor relationships between survival and population productivity

The first 20 months of the COVID-19 pandemic: Mortality, intubation and ICU rates among 104,590 patients hospitalized at 21 United States health systems

Main objective: There is limited information on how patient outcomes have changed during the COVID-19 pandemic. This study characterizes changes in mortality, intubation, and ICU admission rates during the first 20 months of the pandemic. Study design and methods: University of Wisconsin researchers collected and harmonized electronic health record data from 1.1 million COVID-19 patients across 21 United States health systems from February 2020 through September 2021. The analysis comprised data from 104,590 adult hospitalized COVID-19 patients. Inclusion criteria for the analysis were: (1) age 18 years or older; (2) COVID-19 ICD-10 diagnosis during hospitalization and/or a positive COVID-19 PCR test in a 14-day window (+/- 7 days of hospital admission); and (3) health system contact prior to COVID-19 hospitalization. Outcomes assessed were: (1) mortality (primary), (2) endotracheal intubation, and (3) ICU admission. Results and significance: The 104,590 hospitalized participants had a mean age of 61.7 years and were 50.4% female, 24% Black, and 56.8% White. Overall risk-standardized mortality (adjusted for age, sex, race, ethnicity, body mass index, insurance status and medical comorbidities) declined from 16% of hospitalized COVID-19 patients (95% CI: 16% to 17%) early in the pandemic (February-April 2020) to 9% (CI: 9% to 10%) later (July-September 2021). Among subpopulations, males (vs. females), those on Medicare (vs. those on commercial insurance), the severely obese (vs. normal weight), and those aged 60 and older (vs. younger individuals) had especially high mortality rates both early and late in the pandemic. ICU admission and intubation rates also declined across these 20 months. Conclusions: Mortality, intubation, and ICU admission rates improved markedly over the first 20 months of the pandemic among adult hospitalized COVID-19 patients although gains varied by subpopulation. These data provide important information on the course of COVID-19 and identify hospitalized patient groups at heightened risk for negative outcomes. Trial registration: ClinicalTrials.gov Identifier:&nbsp;NCT04506528&nbsp;(https://clinicaltrials.gov/ct2/show/NCT04506528).</p

Experimental active control of a typical section using a trailing-edge flap

This paper presents an experimental implementation of an active control system used to suppress flutter in a typical section airfoil. The H2 optimal control system design is based on experimental system identifications of the transfer functions between three measured system variables - pitch, plunge, and flap position - and a single control signal that commands the flap of the airfoil. Closed-loop response of the airfoil demonstrated gust alleviation below the open-loop flutter boundary. In addition, the flutter boundary was extended by 12.4% through the application of active control. Cursory robustness tests demonstrate stable control for variations in flow speed of ± 10%