Implementation of the Chicago Public Schools Service Learning Initiative: The Role of Teacher Coaches

The growth of service leaming during the past two decades is testimony to this method by which young people learn and develop through active participation in service experiences. Legislative initiatives have supported schools in their efforts to involve youth with their local communities through service linked to academic study. In 1984, 9 percent of all high schools offered some form of service learning. By 1999, 46 percent of public high schools alone were using service learning activities (Kleiner and Chapman 1999). At the same time, service learning studies are complicated by the subject\u27s broad-based goals, the varying contexts and age groups employed, and outcomes often difficult to measure

Supporting Writing Skills in the Common Core State Standards for Students in Grades 3-5 with Executive Functioning and Learning Difficulties: A Teacher’s Toolkit

Poor writers do not display the skills and strategies employed by skilled writers. The Common Core State Standards (CCSS) set forth an expectation that all children will become skilled writers; yet, for most students with executive functioning weakness and/or learning disabilities, writing is the most challenging academic task. For poor writers to begin to develop essential skills for college and career readiness and the willingness to write, teachers need to provide students with specialized materials and teach instructional strategies that students can utilize independently. The purpose of this thesis was to create a toolkit for grade 3-5 teachers containing a range of instructional tools and strategies that target the needs of struggling writers challenged to master the CCSS writing standards

Imagining International Justice in Post-Genocide Cambodia (abstract)

Through an innovative student-faculty collaborative research externship program supported by the Ohio University Center for Law, Justice & Culture, several undergraduate students spent the summer of 2014 in Cambodia conducting independent ethnographic research on issues of law, memory, and justice in the aftermath of the Khmer Rouge genocide. Utilizing the students’ research in Cambodia, this proposed panel session presents three case studies for a conversation regarding how ethnographic methods can inform transitional justice mechanisms by emphasizing local experiences. Much of the research is in light of the Extraordinary Chambers in the Courts of Cambodia (ECCC), a hybrid tribunal that began in 2007 to try senior leaders of the Khmer Rouge and those deemed most responsible for crimes committed between 1975 and 1979. Collectively, the projects explore connections between international justice and contemporary Cambodian society, including how international justice mechanisms produce global legal consciousness, how this consciousness is claimed and contested by local actors, and how legal categories shape collective identity, memories of the past, and imaginations of the future. The research projects reveal a spectrum of issues related to law, memory, and justice. One of the projects discusses the politics of the word “genocide” as it is used in the Cambodian case, drawing upon the experiences and perceptions of the Cham Muslim minority and Case 002/02 of the ECCC. Another project explores representations of victimhood at the Tuol Sleng Museum of Genocidal Crimes as they manifest in debates surrounding a new, ECCC reparations-related memorial on the site. The last project investigates the politics of the cultural production, representation, and translation in genocide exhibitions at three contrasting memorial museums across Cambodia

Perceptual processing deficits underlying reduced FFOV efficiency in older adults

Older adults are known to perform more poorly on measures of the functional field of view (FFOV) than younger adults. Specific contributions by poor bottomup and or top-down control of visual attention to the reduced FFOV of older adults were investigated. Error rates of older and younger adults were compared on a FFOV task in which a central identification task, peripheral localization task, and peripheral distractors were presented in high and low contrast. Older adults made more errors in all conditions. The effect of age was independent of the contrast of the peripheral target or distractors. The performance cost of including the central task was measured and found to be negligible for younger adults. For older adults performance costs were present in all conditions, greater with distractors than without, and greater for a low rather than high contrast central stimulus when the peripheral target was high contrast. These results are consistent with older adults compensating for reduced sensory input or bottom-up capture of attention by relying more heavily on topdown control for which they are resource limited

Inactivation of the Huntington's Disease Gene (Hdh) Impairs Anterior Streak Formation and Early Patterning of the Mouse Embryo

Background: Huntingtin, the HD gene encoded protein mutated by polyglutamine expansion in Huntington's disease, is required in extraembryonic tissues for proper gastrulation, implicating its activities in nutrition or patterning of the developing embryo. To test these possibilities, we have used whole mount in situ hybridization to examine embryonic patterning and morphogenesis in homozygous Hdhex4/5 huntingtin deficient embryos. Results: In the absence of huntingtin, expression of nutritive genes appears normal but E7.0–7.5 embryos exhibit a unique combination of patterning defects. Notable are a shortened primitive streak, absence of a proper node and diminished production of anterior streak derivatives. Reduced Wnt3a, Tbx6 and Dll1 expression signify decreased paraxial mesoderm and reduced Otx2 expression and lack of headfolds denote a failure of head development. In addition, genes initially broadly expressed are not properly restricted to the posterior, as evidenced by the ectopic expression of Nodal, Fgf8 and Gsc in the epiblast and T (Brachyury) and Evx1 in proximal mesoderm derivatives. Despite impaired posterior restriction and anterior streak deficits, overall anterior/posterior polarity is established. A single primitive streak forms and marker expression shows that the anterior epiblast and anterior visceral endoderm (AVE) are specified. Conclusion: Huntingtin is essential in the early patterning of the embryo for formation of the anterior region of the primitive streak, and for down-regulation of a subset of dynamic growth and transcription factor genes. These findings provide fundamental starting points for identifying the novel cellular and molecular activities of huntingtin in the extraembryonic tissues that govern normal anterior streak development. This knowledge may prove to be important for understanding the mechanism by which the dominant polyglutamine expansion in huntingtin determines the loss of neurons in Huntington's disease

Developmental expression patterns ofTbx1,Tbx2,Tbx5, andTbx20 inXenopus tropicalis

T-box genes have diverse functions during embryogenesis and are implicated in several human congenital disorders. Here, we report the identification, sequence analysis, and developmental expression patterns of four members of the T-box gene family in the diploid frog Xenopus tropicalis. These four genes—Tbx1, Tbx2, Tbx5, and Tbx20—have been shown to influence cardiac development in a variety of organisms, in addition to their individual roles in regulating other aspects of embryonic development. Our results highlight the high degree of evolutionary conservation between orthologs of these genes in X. tropicalis and other vertebrates, both at the molecular level and in their developmental expression patterns, and also identify novel features of their expression. Thus, X. tropicalis represents a potentially valuable vertebrate model in which to further investigate the functions of these genes through genetic approaches

Personal health and nutrition information-seeking attitudes and behaviours of first year Canadian and United States veterinary students

Objective: To identify the primary sources of information first year Canadian and US veterinary students relied on for their personal health and nutrition information, and to explore their attitudes towards, and perceptions of, health information resources. Background: Though the animal health information-seeking behaviours (HISB) of veterinary students have been explored, research regarding personal HISB of this professional student population is limited. Evidentiary value: Participants were first year veterinary students (n=322) at the five Canadian veterinary schools and five randomly selected US veterinary schools. An online questionnaire was used to gather students’ demographic information, sources of health and nutrition information, and information-seeking attitudes and perceptions. This study may impact practice at the institutional level for veterinary educators. Methods: STATA 15.1© was used for quantitative analysis; involving multivariate logistic regression models, univariate analyses, and measures of frequency. Results: Results indicated high reliance on the Internet for personal health 213/322 (66%) and nutrition 196/322 (61%) information. While respondents revealed high trust levels in dietary recommendations from family doctors, 132/322 (41%) of students revealed their doctor did not provide any information on healthy diets. Students who reported the use of peer-reviewed journal articles for personal nutrition information were at greater odds of having confidence in knowing where to find nutrition information (Odds Ratio [OR] = 6.61, p<0.001). Conclusion: Participating students reported a high reliance on the Internet search engine Google, and a general lack of guidance from medical professionals regarding general health needs. Application: Veterinary schools should consider this information to enhance student information literacy skills, particularly to facilitate personal HISB, and consequently help in management of personal health throughout the growing demands of the programme

Bioluminescence imaging of G protein-coupled receptor activation in living mice

G protein-coupled receptors (GPCRs), a superfamily of cell-surface receptors involved in virtually all physiological processes, are the major target class for approved drugs. Imaging GPCR activation in real time in living animals would provide a powerful way to study their role in biology and disease. Here, we describe a mouse model that enables the bioluminescent detection of GPCR activation in real time by utilizing the clinically important GPCR, sphingosine-1-phosphate receptor 1 (S1P1). A synthetic S1P1 signaling pathway, designed to report the interaction between S1P1 and β-arrestin2 via the firefly split luciferase fragment complementation system, is genetically encoded in these mice. Upon receptor activation and subsequent β-arrestin2 recruitment, an active luciferase enzyme complex is produced, which can be detected by in vivo bioluminescence imaging. This imaging strategy reveals the dynamics and spatial specificity of S1P1 activation in normal and pathophysiologic contexts in vivo and can be applied to other GPCRs

Confocal microscopy analysis reveals that only a small proportion of extracellular vesicles are successfully labelled with commonly utilised staining methods

Assessing genuine extracellular vesicle (EV) uptake is crucial for understanding the functional roles of EVs. This study measured the bona fide labelling of EVs utilising two commonly used fluorescent dyes, PKH26 and C5-maleimide-Alexa633. MCF7 EVs tagged with mEmerald-CD81 were isolated from conditioned media by size exclusion chromatography (SEC) and characterised using Nanoparticle Tracking Analysis (NTA), Transmission Electron Microscopy (TEM), MACsPlex immunocapture assay and immunoblots. These fluorescently tagged EVs were subsequently stained with C5-maleimide-Alexa633 or PKH26, according to published protocols. Colocalisation of dual-labelled EVs was assessed by confocal microscopy and quantified using the Rank-Weighted Colocalisation (RWC) algorithm. We observed strikingly poor colocalisation between mEmerald-CD81-tagged EVs and C5-Maleimide-Alexa633 (5.4% ± 1.8) or PKH26 (4.6% ± 1.6), that remained low even when serum was removed from preparations. Our data confirms previous work showing that some dyes form contaminating aggregates. Furthermore, uptake studies showed that maleimide and mEmerald-CD81-tagged EVs can be often located into non-overlapping subcellular locations. By using common methods to isolate and stain EVs we observed that most EVs remained unstained and most dye signal does not appear to be EV associated. Our work shows that there is an urgent need for optimisation and standardisation in how EV researchers use these tools to assess genuine EV signals