Can managed grasslands enhance pollinators in intensively farmed areas?

Wild flower strips is a common agri-environmental scheme used by farmers and land managers in order to improve biodiversity of pollinators. However, managed grasslands may also provide flower resources for flower visiting insects in agricultural landscapes. Botanically diverse grasslands on arable farms may support a range of wild pollinators, enhancing pollination services of crops. Intensively managed leys, on the other hand, typically contain only a few high-yielding, competitively strong species. One of the aims of the Multiplant project (2014-2018) was to test perennial seed mixtures targeted for bio-energy, feed protein and biodiversity, in order to develop multi-functional seed mixtures for grasslands. In the current study, we specifically investigated if yield (biomass production) and floral resources for pollinators could be simultaneously optimized by varying botanical composition of mixtures and cutting frequency. We tested four different perennial seed mixtures (3-, 5-, 11- and 13-species mixtures) at three sites varying in surrounding environment using three cutting strategies (no cutting, two cuts per year, four cuts per year). We measured flower production during the season, composition of flower-visitors (in functional groups), and biomass production of all plant species in the seed mixtures. The 11- and 13-species mixtures, which were designed to enhance pollinators, produced similar or higher yield than the 3- and 5- species mixtures under certain cutting regimes. The 3- and 5- species mixtures had a high accumulated flower abundance due to excessive flowering of lucerne under the two-cut strategy and white clover under the four-cut strategy. However, the 11- and 13 species mixtures presented a higher diversity of flowers during the flowering season. Interestingly, accumulated flower abundance was not significantly reduced under the two-cut strategy compared to no cut. Pollinator profiles (visits by different functional groups of insects) were plant-species specific, i.e. at all sites, plant species attracted similar types of insects. Legume species mainly attracted large bees (honey bees and bumblebees), while herbs attracted other insect groups, in particular syrphids and other flies. Our results suggest that multi-species grassland mixtures can be designed to support a higher diversity of pollinators without compromising herbage yield. In particular, adding forbs to the grass-legume mixtures and using a two-cut strategy rather than four cuts per year, may increase flower resources available for a larger range of wild pollinators

Soil nutrient levels define herbage yield but not root biomass in a multispecies grass-legume ley

The response of above- and below-ground biomass to soil nutrient availability is crucial for estimating belowground carbon input and predicting changes in soil carbon storage. However, the response is far from clear at plant community level, especially for grassland systems. Using a long-term field experiment initiated 123 years ago with varying soil nutrient levels (deficient, sub-optimal, optimal and over-optimal) established by use of two nutrient sources (animal manure or mineral fertiliser), we examined the effects of soil nutrient level and source on herbage yield and composition, root biomass and root-to-shoot (R/S) ratio of an unfertilised multispecies grass-legume ley. Increased nutrient levels enhanced herbage yield, but did not affect root biomass. The R/S ratio decreased from deficient to sub-optimal level, but remained constant from optimal to over-optimal level. Nutrient source did not influence herbage yield, root biomass or R/S ratio, but the legume proportion increased in soils previously receiving mineral fertiliser. The R/S ratio decreased with herbage yield, but did not vary with herbage composition. We conclude that soil nutrient level and herbage yield rather than nutrient source and herbage composition determine biomass allocation between aboveground and belowground in temperate grassland leys

Diversity promotes production of ryegrass-clover leys through inclusion of competitive forb species

Highly productive temporary grasslands in Europe are usually composed of only a few plant species, and are typically dominated by perennial ryegrass-clover mixtures. Including additional competitive forb species holds potential for enhancing productivity in temporary grasslands, but requires further demonstration. In a grassland biodiversity experiment, one or all of the three forb species: chicory (Cichorium intybus L.), caraway (Carum carvi L.) and plantain (Plantago lanceolata L.), were grown in different proportions with the perennial ryegrass (Lolium perenne L.)-red clover (Trifolium pratense L.)mixture under two slurry application levels (0 and 250 kg total N ha-1 year-1). Dry matter (DM) yield and botanical composition were determined in 2014 and 2015. Results showed that plantain-containing mixtures significantly increased DM yield by on average 9.5% (20% plantain in seed mixture) to 13.6% (60% plantain) compared to the ryegrass-clover mixture, while other mixtures with forb species produced yields similar to that of the ryegrass-clover mixture. These effects were independent of slurry application and consistent over two years. Moreover, plantain-containing mixtures produced higher yield than chicory- and caraway-containing mixtures, through greater biomass of plantain and/or complementary effects on red clover. These findings firstly demonstrate that increasing species diversity through including certain competitive forbs promotes production of ryegrass-clover mixtures

Differential Phase-contrast Interior Tomography

Differential phase contrast interior tomography allows for reconstruction of a refractive index distribution over a region of interest (ROI) for visualization and analysis of internal structures inside a large biological specimen. In this imaging mode, x-ray beams target the ROI with a narrow beam aperture, offering more imaging flexibility at less ionizing radiation. Inspired by recently developed compressive sensing theory, in numerical analysis framework, we prove that exact interior reconstruction can be achieved on an ROI via the total variation minimization from truncated differential projection data through the ROI, assuming a piecewise constant distribution of the refractive index in the ROI. Then, we develop an iterative algorithm for the interior reconstruction and perform numerical simulation experiments to demonstrate the feasibility of our proposed approach

Starburst in the Intragroup Medium of Stephan's Quintet

Based on new ISO mid-infrared observations and ground based $H_\alpha$ and near-infrared observations, we report the detection of a bright starburst in the intragroup medium (IGM) of the famous compact group of galaxies Stephan's Quintet (Source A in Fig.1). We demonstrate that this starburst is caused by a collision between a high velocity ($\delta$V$\sim$ 1000 km/sec) intruder galaxy (NGC7318b) and the IGM of the group. While this is the only starburst known today that is induced by a galaxy/cold-intergalactic-medium collision, it provides new constraints to the theory for interaction-induced starbursts, and may hint at a new mechanism for the star formation excess seen in more distant clusters.Comment: 17 pages, 2 PS figures. Accepted by Ap

Forbs enhance productivity of unfertilised grass-clover leys and support low-carbon bioenergy

Intensively managed grasslands are dominated by highly productive grass-clover mixtures. Increasing crop diversity by inclusion of competitive forbs may enhance biomass production and sustainable biofuel production. Here we examined if one or all of three forbs (chicory, Cichorium intybus L.; caraway, Carum carvi L.; plantain, Plantago lanceolata L.) included in ryegrass-red clover mixtures enhanced above- and below-ground productivity, and assessed their biofuel potentials, based on a three-year experiment with and without fertilisation as cattle slurry. We determined herbage yield, standing root biomass, and estimated methane energy output and greenhouse gas (GHG) emissions per energy unit using life cycle assessment. Results showed that plantain-containing grass-clover mixtures significantly increased herbage yield, while chicory- or caraway-containing mixtures maintained similar yields to the grass-clover mixture. Standing root biomass of the grass-clover mixture was enhanced by inclusion of caraway and plantain, with that of plantain further enhanced by fertilisation. The highest methane energy output was achieved in plantain-containing grass-clover mixtures. All unfertilised mixtures achieved the 60% reduction in GHG emissions compared to fossil fuel, whereas all fertilised mixtures did not meet the 60% reduction target. These findings suggest that including competitive forbs such as plantain in grass-clover mixtures enhances productivity, supporting low-carbon footprint bioenergy production

Kløvergræsblandinger med urter - kulstof, kvælstof og biodiversitet

Urter kan bidrage på en række punkter – bl.a. øget biodiversitet, kulstoflagring, bedre ressourceudnyttelse og øget udbytte. Men det er vigtigt at kontrollere andelen

Anaerobic co-digestion of grass and forbs – Influence of cattle manure or grass based inoculum

Anaerobic co-digestion of agricultural by-products or wastes with complementarity characteristics is commonly used to enhance methane yield. This study firstly explores the possibility of co-digesting grass and forb species (white clover, chicory and plantain) differing in nutrient composition in enhancing methane yield. This was examined with two inocula (a cattle manure-based inoculum and a grass-based inoculum) in a batch assay. Results showed that co-digesting grass and forbs synergistically enhanced methane yield potential on average by 31 L kg−1 volatile solids (+11%) and reduced lag phase time by 0.8 day in the grass-based inoculum, but not in the cattle manure-based inoculum. Mixtures containing plantain showed more consistent synergistic effect than chicory. Synergistic effects were attributed to more balanced nutrient composition (especially C/N ratio) in grass-forb mixtures. We demonstrate that anaerobic co-digestion of grass and forbs is feasible for enhancing methane yield, which promotes the utilization of multi-species grasslands for bioenergy production