A conserved strategy for inducing appendage regeneration

Can limb regeneration be induced? Few have pursued this question, and an evolutionarily conserved strategy has yet to emerge. This study reports a strategy for inducing regenerative response in appendages, which works across three species that span the animal phylogeny. In Cnidaria, the frequency of appendage regeneration in the moon jellyfish Aurelia was increased by feeding with the amino acid L-leucine and the growth hormone insulin. In insects, the same strategy induced tibia regeneration in adult Drosophila. Finally, in mammals, L-leucine and sucrose administration induced digit regeneration in adult mice, including dramatically from mid- phalangeal amputation. The conserved effect of L-leucine and insulin/sugar suggests a key role for energetic parameters in regeneration induction. The simplicity by which nutrient supplementation can induce appendage regeneration provides a testable hypothesis across animals

Regeneration data - Drosophila

Abrams et al., 2020 (https://doi.org/10.1101/2020.11.21.392720). This dataset contains the raw videos analyzed and quantified for generating Figure 5 and the associated supplementary figures. See the source file Drosophila Data.xls for magnification information.Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/</p>Exp0619.zip 9.9 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0619.zip" > <i class="download icon"></i> Download </a></p> Exp0623-1.zip 4.6 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0623-1.zip" > <i class="download icon"></i> Download </a></p> Exp0623-2.zip 3.8 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0623-2.zip" > <i class="download icon"></i> Download </a></p> Exp0626.zip 9.1 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0626.zip" > <i class="download icon"></i> Download </a></p> Exp0629.zip 3.9 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0629.zip" > <i class="download icon"></i> Download </a></p> Exp0630.zip 4.3 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0630.zip" > <i class="download icon"></i> Download </a></p> Exp0706.zip 4.6 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0706.zip" > <i class="download icon"></i> Download </a></p> Exp0708.zip 4.0 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0708.zip" > <i class="download icon"></i> Download </a></p> Exp0709.zip 4.3 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0709.zip" > <i class="download icon"></i> Download </a></p> Exp0713.zip 8.1 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0713.zip" > <i class="download icon"></i> Download </a></p> Exp0719.zip 1.9 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0719.zip" > <i class="download icon"></i> Download </a></p> Exp0720.zip 6.0 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0720.zip" > <i class="download icon"></i> Download </a></p> Exp0722.zip 7.6 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0722.zip" > <i class="download icon"></i> Download </a></p> Exp0730-2.zip 7.1 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0730-2.zip" > <i class="download icon"></i> Download </a></p> Exp0730.zip 3.6 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0730.zip" > <i class="download icon"></i> Download </a></p> Exp0802.zip 2.0 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0802.zip" > <i class="download icon"></i> Download </a></p> Exp0806.zip 1.4 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0806.zip" > <i class="download icon"></i> Download </a></p> Exp0829.zip 4.3 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0829.zip" > <i class="download icon"></i> Download </a></p> Exp0903.zip 5.5 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0903.zip" > <i class="download icon"></i> Download </a></p> Exp0908.zip 6.0 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp0908.zip" > <i class="download icon"></i> Download </a></p> Exp1858.zip 5.6 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp1858.zip" > <i class="download icon"></i> Download </a></p> Exp3867.zip 5.5 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/Exp3867.zip" > <i class="download icon"></i> Download </a></p> ExpAAS.zip 1.9 GB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/ExpAAS.zip" > <i class="download icon"></i> Download </a></p> ExpZC.zip 634.3 MB <a role="button" class="ui compact mini button" href="https://renc.osn.xsede.org/ini210004tommorrell/0_lgoentor/ExpZC.zip" > <i class="download icon"></i> Download </a></p&gt

Response to comment on 'A conserved strategy for inducing appendage regeneration in moon jellyfish, Drosophila, and mice'

Previously we reported evidence that a regenerative response in the appendages of moon jellyfish, fruit flies, and mice can be promoted by nutrient modulation (Abrams et al., 2021). Sustar and Tuthill subsequently reported that they had not been able to reproduce the induced regenerative response in flies (Sustar and Tuthill, 2023). Here we discuss that differences in the amputation method, treatment concentrations, age of the animals, and stress management explain why they did not observe a regenerative response in flies. Typically, 30–50% of treated flies showed response in our assay

Response to comment on 'A conserved strategy for inducing appendage regeneration in moon jellyfish, Drosophila, and mice'.

Previously we reported evidence that a regenerative response in the appendages of moon jellyfish, fruit flies, and mice can be promoted by nutrient modulation (Abrams et al., 2021). Sustar and Tuthill subsequently reported that they had not been able to reproduce the induced regenerative response in flies (Sustar and Tuthill, 2023). Here we discuss that differences in the amputation method, treatment concentrations, age of the animals, and stress management explain why they did not observe a regenerative response in flies. Typically, 30-50% of treated flies showed response in our assay

A conserved strategy for inducing appendage regeneration in moon jellyfish, Drosophila, and mice

Can limb regeneration be induced? Few have pursued this question, and an evolutionarily conserved strategy has yet to emerge. This study reports a strategy for inducing regenerative response in appendages, which works across three species that span the animal phylogeny. In Cnidaria, the frequency of appendage regeneration in the moon jellyfish Aurelia was increased by feeding with the amino acid L-leucine and the growth hormone insulin. In insects, the same strategy induced tibia regeneration in adult Drosophila. Finally, in mammals, L-leucine and sucrose administration induced digit regeneration in adult mice, including dramatically from mid-phalangeal amputation. The conserved effect of L-leucine and insulin/sugar suggests a key role for energetic parameters in regeneration induction. The simplicity by which nutrient supplementation can induce appendage regeneration provides a testable hypothesis across animals

A conserved strategy for inducing appendage regeneration in moon jellyfish, Drosophila, and mice

Can limb regeneration be induced? Few have pursued this question, and an evolutionarily conserved strategy has yet to emerge. This study reports a strategy for inducing regenerative response in appendages, which works across three species that span the animal phylogeny. In Cnidaria, the frequency of appendage regeneration in the moon jellyfish Aurelia was increased by feeding with the amino acid L-leucine and the growth hormone insulin. In insects, the same strategy induced tibia regeneration in adult Drosophila. Finally, in mammals, L-leucine and sucrose administration induced digit regeneration in adult mice, including dramatically from mid-phalangeal amputation. The conserved effect of L-leucine and insulin/sugar suggests a key role for energetic parameters in regeneration induction. The simplicity by which nutrient supplementation can induce appendage regeneration provides a testable hypothesis across animals