31 research outputs found
Atypical BSE (BASE) Transmitted from Asymptomatic Aging Cattle to a Primate
BACKGROUND: Human variant Creutzfeldt-Jakob Disease (vCJD) results from foodborne transmission of prions from slaughtered cattle with classical Bovine Spongiform Encephalopathy (cBSE). Atypical forms of BSE, which remain mostly asymptomatic in aging cattle, were recently identified at slaughterhouses throughout Europe and North America, raising a question about human susceptibility to these new prion strains. METHODOLOGY/PRINCIPAL FINDINGS: Brain homogenates from cattle with classical BSE and atypical (BASE) infections were inoculated intracerebrally into cynomolgus monkeys (Macacca fascicularis), a non-human primate model previously demonstrated to be susceptible to the original strain of cBSE. The resulting diseases were compared in terms of clinical signs, histology and biochemistry of the abnormal prion protein (PrPres). The single monkey infected with BASE had a shorter survival, and a different clinical evolution, histopathology, and prion protein (PrPres) pattern than was observed for either classical BSE or vCJD-inoculated animals. Also, the biochemical signature of PrPres in the BASE-inoculated animal was found to have a higher proteinase K sensitivity of the octa-repeat region. We found the same biochemical signature in three of four human patients with sporadic CJD and an MM type 2 PrP genotype who lived in the same country as the infected bovine. CONCLUSION/SIGNIFICANCE: Our results point to a possibly higher degree of pathogenicity of BASE than classical BSE in primates and also raise a question about a possible link to one uncommon subset of cases of apparently sporadic CJD. Thus, despite the waning epidemic of classical BSE, the occurrence of atypical strains should temper the urge to relax measures currently in place to protect public health from accidental contamination by BSE-contaminated products
Synthesis of 3-Amino-3-Aminomethyl-2H-1-Benzopyran and Spiro[1-Benzopyran-3(2H),2'- Piperazine] Derivatives
International audienc
New efficient access to thieno[3,2-b]pyridine derivatives via regioselective lithiation of 3-methylthiopyridine
International audienc
Toolbox for Regioselective Lithiations of Furo[2,3- c ]pyridine
International audienc
Synthesis of N-Substituted Imidoethyl-3-aminomethyl-2,3-dihydro-1,4-dioxino[2,3-b]pyridines
International audienc
One-pot double functionalisation of π-deficient heterocyclic lithium reagents
International audienc
Enantioselective synthesis of (+)-3-oxabicyclo[3.2.0]hept-6-en-2-one
International audienc
A New Synthesis of cis-Cyclobut-3-ene-1,2-dicarboxylic Anhydride
International audienc
Successive Regioselective Metalations of Fused Heterocycles: Synthesis of Polyfunctionalized Furo[3,2- b ]pyridines
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