Anti-phosphorylcholine antibodies of the T15 idiotype are optimally protective against Streptococcus pneumoniae

Since their initial discovery (1) immunoglobulin idiotypes have proven to be useful in studies examining antibody diversity, inheritance of variable region expression, regulation of specific antibody responses, and the possible use of antibody heavy chain variable (Vrz) 1 regions in T cell antigen receptors. In the present report, we present evidence that there is a close relationship between the idiotype of an anti-phosphorylcholine (PC) antibody and its ability to protect mice from pneumococcal infection. These findings extend idiotypic analysis into the area of microbial pathogenesis and provide added relevance to previous studies of the regulation of idiotype expression, and the evolution of immunoglobulin (Ig) V region genes. Murine anti-PC antibodies and myelomas fall into three discrete families bearing either the T15, M603, or M511 idiotype (2-4). Antibodies within each of these families have fine specificities for PC analogues and PC-carrier conjugates that are characteristic of their respective families (3, 4). These three idiotype families, however, are very closely related, and their heavy chains are thought to be transcribed from the same rearranged Vn, D, and J region genes (5). Each of the three idiotype families i

The effects of idiotype on the ability of IgG 1 antiphosphorylcholine antibodies to protect mice from fatal infection with Streptococcus pneumoniae

Anti-phosphorylcholine (PC) antibodies of the mouse are found in three different idiotype families: T15, M603 and M511. These subgroups exhibit different specificities for PC analogs and utilize light chains of different V L subgroups. In this study we have found that IgG 1 antibodies of the T15 idiotype are much more protective against pneumococcal infection than IgG 1 antibodies of the M511 or M603 idiotypes. This finding provides additional evidence that the T15 V H and V L genes may have evolved to protect mice from infection with PC-bearing pathogens.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/37959/1/1830141112_ftp.pd

Mouse IgG3 antibodies are highly protective against infection with Streptococcus pneumoniae

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/62976/1/294088a0.pd