1,289 research outputs found
Sedimentology and kinematics of a large, retrogressive growth-fault system in Upper Carboniferous deltaic sediments, western Ireland
Growth faulting is a common feature of many deltaic environments and is vital in determining local sediment dispersal and accumulation, and hence in controlling the resultant sedimentary facies distribution and architecture. Growth faults occur on a range of scales, from a few centimetres to hundreds of metres, with the largest growth faults frequently being under-represented in outcrops that are often smaller than the scale of feature under investigation. This paper presents data from the exceptionally large outcrops of the Cliffs of Moher, western Ireland, where a growth-fault complex affects strata up to 60 m in thickness and extends laterally for 3 km. Study of this Namurian (Upper Carboniferous) growth-fault system enables the relationship between growth faulting and sedimentation to be detailed and permits reconstruction of the kinematic history of faulting. Growth faulting was initiated with the onset of sandstone deposition on a succession of silty mudstones that overlie a thin, marine shale. The decollement horizon developed at the top of the marine shale contact for the first nine faults, by which time aggradation in the hangingwall exceeded 60 m in thickness. After this time, failure planes developed at higher stratigraphic levels and were associated with smaller scale faults. The fault complex shows a dominantly landward retrogressive movement, in which only one fault was largely active at any one time. There is no evidence of compressional features at the base of the growth faults, thus suggesting open-ended slides, and the faults display both disintegrative and non-disintegrative structure. Thin-bedded, distal mouth bar facies dominate the hangingwall stratigraphy and, in the final stages of growth-fault movement, erosion of the crests of rollover structures resulted in the highest strata being restricted to the proximity of the fault. These upper erosion surfaces on the fault scarp developed erosive chutes that were cut parallel to flow and are downlapped by the distal hangingwall strata of younger growth faults
Refined measurement of SecA-driven protein secretion reveals that translocation is indirectly coupled to ATP turnover
The universally conserved Sec system is the primary method cells utilize to transport proteins across membranes. Until recently, measuring the activity—a prerequisite for understanding how biological systems work—has been limited to discontinuous protein transport assays with poor time resolution or reported by large, nonnatural tags that perturb the process. The development of an assay based on a split superbright luciferase (NanoLuc) changed this. Here, we exploit this technology to unpick the steps that constitute posttranslational protein transport in bacteria. Under the conditions deployed, the transport of a model preprotein substrate (proSpy) occurs at 200 amino acids (aa) per minute, with SecA able to dissociate and rebind during transport. Prior to that, there is no evidence for a distinct, rate-limiting initiation event. Kinetic modeling suggests that SecA-driven transport activity is best described by a series of large (∼30 aa) steps, each coupled to hundreds of ATP hydrolysis events. The features we describe are consistent with a nondeterministic motor mechanism, such as a Brownian ratchet
Particle dynamics of a cartoon dune
The spatio-temporal evolution of a downsized model for a desert dune is
observed experimentally in a narrow water flow channel. A particle tracking
method reveals that the migration speed of the model dune is one order of
magnitude smaller than that of individual grains. In particular, the erosion
rate consists of comparable contributions from creeping (low energy) and
saltating (high energy) particles. The saltation flow rate is slightly larger,
whereas the number of saltating particles is one order of magnitude lower than
that of the creeping ones. The velocity field of the saltating particles is
comparable to the velocity field of the driving fluid. It can be observed that
the spatial profile of the shear stress reaches its maximum value upstream of
the crest, while its minimum lies at the downstream foot of the dune. The
particle tracking method reveals that the deposition of entrained particles
occurs primarily in the region between these two extrema of the shear stress.
Moreover, it is demonstrated that the initial triangular heap evolves to a
steady state with constant mass, shape, velocity, and packing fraction after
one turnover time has elapsed. Within that time the mean distance between
particles initially in contact reaches a value of approximately one quarter of
the dune basis length
Research encounters, reflexivity and supervision
Reflexivity in qualitative and ethnographic social science research can provide a rich source of data, especially regarding the affective, performative and relational aspects of interviews with research subjects. This paper explores by means of three case examples different ways of accessing and using such reflexivity. The examples are drawn from an empirical psycho-social study into the identity transitions of first-time mothers in an inner-city multicultural environment. Fieldnotes and supervision were used to engage with researcher subjectivity, to enhance the productive use of reflexivity and to address the emotional work of research. The methodology of the supervision was psychoanalytic, in its use of a boundaried frame and of psychoanalytic forms of noticing oneself, of staying engaged emotionally as well as creating a reflective distance. The examples illustrate how this can enhance the knowledge gained about the research subjects
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