182 research outputs found
'Singing Songs, Making Places, Creating Selves:' Football Songs & Fan Identity at Sydney FC
The Australian A-League soccer competition was established in 2004. The creation of a new national soccer league precipitated many changes within Australiaâs football culture. These changes were particularly difficult for the supporters because, with a single exception, all the A-League teams were completely new âfranchisesâ. The reinvented competition required soccer fans to adopt a new team, to develop new loyalties, new rituals, new places, and consequently a new fan identity. Vital to this act of re-creation has been the collective authorship of a ânewâ repertoire of football songs. Football songs and communal singing are central to the traditions and performance of soccer fandom. Football song plays a key, perhaps even determining, role in the creation of fan identity. In this paper I examine the way football songs are used create a fan identity for Sydneyâs new A-League side: Sydney FC. I argue that the result of Sydney fansâ conscious act of cultural creation is a repertoire of songs and chants that, although derived from an increasingly globalised and commodified football culture, is able to articulate a local identity. Moreover, these songs may even be thought to articulate a local and a global fan identity simultaneously, as fans connect local and distant spaces within global soccer culture
SecA â a new twist in the tail
A paper published in this issue of the Journal of Bacteriology (D. Huber, M. Jamshad, R. Hanmer, D. Schibich, K. Döring, I. Marcomini, G. Kramer, and B. Bukau, J Bacteriol 199:e0622-16, 2017, https://doi.org/10.1128/JB.00622-16) provides us with a timely reminder that all is not as clear as we had previously thought in the general bacterial secretion system. The paper describes a new mode of secretion through the Sec systemââuncoupled cotranslocationââfor the passage of proteins across the bacterial inner membrane and suggests that we might rethink the nature and mechanism of the targeting and transport steps toward protein export
Application of enhanced thermal ratings to primary substation transformers
The use of thermal modelling to increase the permissible load-carrying capability of distribution system transformers is attracting increasing interest. Many reported approaches calculate the rating of transformers in real-time in response to system conditions. In this paper, we describe an experiment to validate and tune the parameters of such a thermal model, and explain how the results have been used to inform the inclusion of model-based seasonal âenhancedâ ratings in the network planning process
Protein translocation:whatâs the problem?
We came together in Leeds to commemorate and celebrate the life and achievements of Prof. Stephen Baldwin. For many years we, together with Sheena Radford and Roman Tuma (colleagues also of the University of Leeds), have worked together on the problem of protein translocation through the essential and ubiquitous Sec system. Inspired and helped by Steve we may finally be making progress. My seminar described our latest hypothesis for the molecular mechanism of protein translocation, supported by results collected in Bristol and Leeds on the tractable bacterial secretion processâcommonly known as the Sec system; work that will be published elsewhere. Below is a description of the alternative and contested models for protein translocation that we all have been contemplating for many years. This review will consider their pros and cons
Refined measurement of SecA-driven protein secretion reveals that translocation is indirectly coupled to ATP turnover
The universally conserved Sec system is the primary method cells utilize to transport proteins across membranes. Until recently, measuring the activityâa prerequisite for understanding how biological systems workâhas been limited to discontinuous protein transport assays with poor time resolution or reported by large, nonnatural tags that perturb the process. The development of an assay based on a split superbright luciferase (NanoLuc) changed this. Here, we exploit this technology to unpick the steps that constitute posttranslational protein transport in bacteria. Under the conditions deployed, the transport of a model preprotein substrate (proSpy) occurs at 200 amino acids (aa) per minute, with SecA able to dissociate and rebind during transport. Prior to that, there is no evidence for a distinct, rate-limiting initiation event. Kinetic modeling suggests that SecA-driven transport activity is best described by a series of large (âŒ30 aa) steps, each coupled to hundreds of ATP hydrolysis events. The features we describe are consistent with a nondeterministic motor mechanism, such as a Brownian ratchet
Widespread tissue hypoxia dysregulates cell and metabolic pathways in SMA
Open Access via the Wiley Jisc Agreement Acknowledgments: SHP, EHâG, INF, SDâA, and JMC were funded by SMA Europe (SMA UK and Prinses Beatrix Spierfonds). Thanks to Prof Andy Welch for helpful discussions on imaging.Peer reviewedPublisher PD
Recommended from our members
Interplay between Mitochondrial Protein Import and Respiratory Complexes Assembly in Neuronal Health and Degeneration.
The fact that >99% of mitochondrial proteins are encoded by the nuclear genome and synthesised in the cytosol renders the process of mitochondrial protein import fundamental for normal organelle physiology. In addition to this, the nuclear genome comprises most of the proteins required for respiratory complex assembly and function. This means that without fully functional protein import, mitochondrial respiration will be defective, and the major cellular ATP source depleted. When mitochondrial protein import is impaired, a number of stress response pathways are activated in order to overcome the dysfunction and restore mitochondrial and cellular proteostasis. However, prolonged impaired mitochondrial protein import and subsequent defective respiratory chain function contributes to a number of diseases including primary mitochondrial diseases and neurodegeneration. This review focuses on how the processes of mitochondrial protein translocation and respiratory complex assembly and function are interlinked, how they are regulated, and their importance in health and disease
- âŠ