Usefulness of PKH fluorescent labelling to study leukemic cell proliferation with various cytostatic drugs or acetyl tetrapeptide – AcSDKP

BACKGROUND: PKH67 labelling was compared for classical proliferation assessment (using S phase evaluation) to analyse the cell proliferation of 29 AML patients treated or not with various drugs. Among these drugs, the effect of tetrapeptide AcSDKP or AcSDKP-NH2 on AML cells, stimulated or not by cytokines, was also evaluated in order to determine (i) if AcSDKP was able to inhibit blast cell proliferation as it inhibits haematopoietic progenitors (ii) if AcSDKP-NH2 was more stable than AcSDKP with FBS. METHODS: For PKH labeling, cells were suspended in Diluent C, and rapidly admixed with PKH67 solution at 20 μM PKH67. Staining was stopped by addition of FBS. RESULTS: A good correlation between PKH67 labelling and bromodeoxyuridine incorporation was obtained first with 6/9 patients for control cells, then for 11/17 AML patients treated with classical antileukemic drugs (among whom 4 were also treated with AcSDKP). The effect of AcSDKP was also studied on 7 patients. The discrepancy between both methods was essentially due to an accumulation of cells into different cycle phases measured by BrdUrd incorporation secondary to drug action and PKH67 labelling which measured the dynamic proliferation. This last method allows identifying resistant cells which still proliferate. AcSDKP or AcSDKP-NH2 induced a decrease of leukemic cell proliferation in 5/7 patients when cytokines were added (in order to stimulate proliferation) one day after tetrapeptide AcSDKP or AcSDKP-NH2. No effect on proliferation was noted when cytokines were added to AcSDKP-NH2. CONCLUSION: PKH67 labelling method is a powerful tool for cell proliferation assessment in patients with AML, even in cells treated by various drugs

FoxO3a overexpression prevents both glycogen overload and autophagic buildup in skeletal muscle of Pompe disease

FoxO3a overexpression prevents both glycogen overload and autophagic buildup in skeletal muscle of Pompe disease. 6eme congrès international de Myologi

Satellite cells fail to contribute to muscle repair but are functional in Pompe disease (glycogenosis type II)

Satellite cells fail to contribute to muscle repair but are functional in Pompe disease (glycogenosis type II). 6eme congrés international de Myologi

Bone Marrow Transplantation for Feline Mucopolysaccharidosis I

Severe mucopolysaccharidosis type I (MPS I) is a fatal neuropathic lysosomal storage disorder with significant skeletal involvement. Treatment involves bone marrow transplantation (BMT), and although effective, is suboptimal, due to treatment sequelae and residual disease. Improved approaches will need to be tested in animal models and compared to BMT. Herein we report on bone marrow transplantation to treat feline mucopolysaccharidosis I (MPS I). Five MPS I stably engrafted kittens, transplanted with unfractionated bone marrow (6.3 × 107–1.1 × 109 nucleated bone marrow cells per kilogram) were monitored for 13–37 months post-engraftment. The tissue total glycosaminoglycan (GAG) content was reduced to normal levels in liver, spleen, kidney, heart muscle, lung, and thyroid. Aorta GAG content was between normal and affected levels. Treated cats had a significant decrease in the brain GAG levels relative to untreated MPS I cats and a paradoxical decrease relative to normal cats. The α-l-iduronidase (IDUA) activity in the livers and spleens of transplanted MPS I cats approached heterozygote levels. In kidney cortex, aorta, heart muscle, and cerebrum, there were decreases in GAG without significant increases in detectable IDUA activity. Treated animals had improved mobility and decreased radiographic signs of disease. However, significant pathology remained, especially in the cervical spine. Corneal clouding appeared improved in some animals. Immunohistochemical and biochemical analysis documented decreased central nervous system ganglioside storage. This large animal MPS I study will serve as a benchmark of future therapies designed to improve on BMT

Cadeia produtiva do gengibre (Zingiber officinale ROSCOE) no estado do Paraná : análise e recomendações para melhoria da qualidade

Orientador : Raquel R.B.NegrelleCo-orientadores: Luiz Doni Filho, Neusa G.A. RuckerTese (doutorado) - Universidade Federal do Paraná. Setor de Ciências Agrárias. Curso de Pós-Graduação em Agronomia - área de concentração Produção Vegetal, Departamento de Fitotecnia e FitossanitarismoInclui bibliografia e glossárioResumo: O presente trabalho teve como objetivo o estudo prospectivo da cadeia produtiva do Zingiber officinale Roscoe no Estado do Paraná, identificando os principais agentes envolvidos e paralelamente proceder análise dos pontos de estrangulamentos, especificamente no que concerne à qualidade microbiológica deste produto em todos os níveis, desde a produção até a fase final da comercialização, visando identificar causas e propor soluções no sentido da melhoria do sistema como um todo. Este estudo foi desenvolvido no período de 2000 a 2002 englobando pesquisa de campo, entrevistas abertas com representantes de estabelecimentos de comercialização, produtores e demais atores da cadeia produtiva, além de análises laboratoriais do produto "in natura" disponível no mercado consumidor da Região Metropolitana de Curitiba - PR. Este documento está organizado em 9 capítulos, que apresentam vastas informações englobando aspectos botânicos, ecológicos, fisico-químicos e farmacológicos do gengibre, e que estão incluídas nos capítulos 1 e 2. O capítulo 3 apresenta características gerais da principal região produtora brasileira, Morretes - PR, englobando localização, aspectos sócio-econômicos, geológicos e geomorfológicos e caracterização climática. O capítulo 4 apresenta o estudo prospectivo da cadeia produtiva do gengibre no Estado do Paraná, que engloba panorama mundial, brasileiro e paranaense do volume de produção agrícola de gengibre. Inclui-se também caracterização da comunidade produtora agrícola do litoral paranaense, além da identificação e caracterização dos outros diferentes níveis da cadeia produtiva do gengibre e a detecção dos principais pontos de estrangulamento nos diferentes níveis desta cadeia produtiva. A caracterização do cultivo e beneficiamento do gengibre no litoral paranaense é apresentada no capítulo 5, evidenciando suas particularidades frente ao descrito na literatura especializada. No capítulo 6 avaliou-se as condições higiênico-sanitárias dos estabelecimentos produtores, do processo de beneficiamento pós-colheita (lavagem, limpeza, secagem e embalagem) e das condições de manipulação do gengibre "in natura" no litoral paranaense. De maneira geral, as condições de higiene e limpeza observadas foram consideradas precárias, determinando alta potencialidade de contaminação do gengibre com agentes que poderiam colocar em risco a saúde do consumidor. No capítulo 7 são apresentados os resultados dos estabelecimentos de comercialização da Região Metropolitana de Curitiba - PR, no que tange à qualidade e adequação às normas vigentes. Dos aspectos observados, as condições dos vestuários e dos equipamentos de proteção individual (manipuladores) foram considerados precários, o que pode potencializar a contaminação do produto e colocar em risco a saúde do consumidor. O capítulo 8 teve como objetivo caracterizar o perfil microbiológico do gengibre "in natura" comercializado na Região Metropolitana de Curitiba - PR, tendo como base a Resolução - CNPPA n. 12 - Brasil, 1978 e a Resolução - RDC n. 12 - Brasil, 2001. Para tanto, foram realizadas a determinação do número mais provável (NMP) de coliformes totais, coliformes a 45° C/g e Escherichia coli, e a presença de Salmonella sp em 25 gramas. Sintetizando, um conjunto de propostas e recomendações aos agentes econômicos que atuam e processam a cadeia produtiva do gengibre no Estado do Paraná, em especial o município de Morretes - PR, é apresentado no capítulo 9Abstract: Prospective studies of the productive chain of the Zingiber officinale Roscoe on Parana State identifying the main agents envolved and parallel procedure analysis of the strangulation points, in order to microbiology qualify this product in whole levels from the production until commercialization last phase, aiming identify causes and propose solutions to the improvement of the system in general. This survey developed from 2000 to 2002 involved field research, interviews with representative of the commercialization establishments, producers and others actors of the productive chain, besides made in laboratories of the quality of crude product available to the consumer market in Curitiba. This paper is organized in nine chapters that present information involving botanical, ecological, physique-chemical and pharmacological aspects of ginger that are included in chapters 1 and 2. The chapter 3 presents the characteristics of the main brazilian productive area, embody localization, social and economic aspects, geologycal, geomorphologycal and climate characterization. The chapter 4 the study of the productive chain of this product in Parana State, which includes the paranaense, the brazilian and the worldwide panoramic view of the production volume and commercialization of the ginger; characterization of the agricultural community; identification and characterization of others different levels of the productive chain and also, the main strangulation points on these different levels of the productive chain. In the chapter 5 the agricultural process of the ginger benefit and cultivation in Morretes Town, Paraná, Brazil was caracterized. In the chapter 6 the hygienic-sanitary conditions of the producers establishments, postharvest benefit system and manipulation conditions of crude ginger in the paranaense coastland, Brazil were evaluated with an aim to subsidy the ofert of the best quality product to consumer market. In general, the cleaning and hygienic conditions were precarious, determining a high potential of ginger contamination with agents that can put in risk the consumers' health. The chapter 7 presents the results of the commercialization establishments in the metropolitan area of Curitiba considering adequation to the current norm. Of the observed items, the manipulation conditions was considered precarious due to the inadequate conditions that contributes to contamination, bringing about serious problems to consumers' health. The chapter 8 aimed the microbiological quality of the ginger market in the metropolitan area of Curitiba based on the Brazilian legislation. The microbiological analysis found total coliforms, 45° C/g coliforms, Escherichia coli most probable number (MPN) and Salmonella sp presence. Synthetizing, a group of considerations and recommendations to economic agents that actuate and carry on the productive chain of the ginger in the Parana State, specially in Morretes Town, is presented in the chapter

Les vétérinaires et le vieillissement cérébral chez le chien (enquête nationale et réalisation d'un document pratique)

Le vieillissement cérébral chez le chien se manifeste par des troubles comportementaux et nerveux, qui sont des motifs de consultation de plus en plus fréquents. Dans une première partie, l'auteur présente l'élaboration et les résultats d'une enquête nationale réalisée auprès des praticiens vétérinaires. Cette étude évalue les connaissances, les pratiques et les sujets d'interrogation des vétérinaires sur le vieillissement cérébral chez le chien. La seconde partie de ce travail a pour objectif d'offrir une réponse didactique à ces interrogations, sous forme de fiches pratiques, comportant les données essentielles sur la pathogénie, la symptomatologie, la conduite diagnostique à tenir et les thérapeutiques utilisables lors du vieillissement cérébral chez le chien.NANTES-Ecole Nat.Vétérinaire (441092302) / SudocSudocFranceF

Etude clinique d'un modèle canin de mucopolysaccharidose de type 7

La mucopolysaccharidose de type 7 (MPS7) est une maladie de surcharge lysosomiale à l origine d une surcharge en glycosaminoglycanes responsable d un dysfonctionnement de la quasi-totalité des cellules de l organisme. Dans un modèle canin de MPS7 nous avons étudié sur 6 mois l évolution de la maladie en nous basant sur des examens cliniques généraux, des examens d imagerie, des analyses biochimiques et un suivi de l audition par méthode électrophysiologique. Le but de cette étude in fine était d identifier des critères cliniques, radiologiques ou biochimiques pertinents pouvant être utilisés comme indicateurs d efficacité thérapeutique. Ces examens ont permis de montrer que les animaux présentent des lésions dès la naissance et d autres qui apparaissent après quelques mois. Les principales lésions observées sont un retard de croissance, des malformations organiques sévères et des troubles ostéo-articulaires. Aucune anomalie électrophysiologique n a été détectée pour l oreille moyenne ou interne. Plusieurs scores cliniques ont été mis en place afin de pouvoir suivre au mieux l évolution de la maladie, ils pourront être utilisés pour de futures études thérapeutiques.NANTES-Ecole Nat.Vétérinaire (441092302) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF

Ophtalmologie des oiseaux de compagnie et des oiseaux sauvages

Les nouveaux animaux de compagnie, dont les oiseaux, connaissent un nombre croissant d'adeptes très demandeurs d'une médecine de pointe analogue à celle développée pour les carnivores. Cet ouvrage a pour but d'apporter au praticien les connaissances et les gestes essentiels nécessaires à la pratique d'une consultation de médecine ophtalmologique aviaire. Après avoir rappelé en introduction la classification des oiseaux de compagnie et celle des oiseaux sauvages, l'auteur souligne dans la première partie les particularités anatomiques, histologiques et physiologiques de l'oeil des oiseaux et de ses annexes. Dans une deuxième partie sont abordés la démarche pratique d'une consultation ophtalmologique aviaire et des cas cliniques. Enfin, la troisième partie, richement illustrée, expose les principales affections oculaires rencontrées en médecine aviaire.NANTES-Ecole Nat.Vétérinaire (441092302) / SudocSudocFranceF

Etude anatomo-clinique des affections neurologiques suspectées génétiques du Rottweiler (guide pratique à l'usage du vétérinaire)

Les affections neurologiques du Rottweiler supposées génétiques se caractérisent par des troubles nerveux centraux ou périphériques ou les deux. Six entités sont envisagées dans cette étude : la leucoencéphalomyélopathie, la dystrophie neuroaxonale, les amyotrophies neurogènes, les neuropathies distales sensorimotrices, le complexe polyneuropathie associée à une paralysie laryngée et la vacuolisation neuronale et la dégénérescence spinocérébelleuse. Le but de cette étude est de faire une revue de la littérature sur ces affections rares et mal connues, ainsi que d apporter au vétérinaire praticien des clés de diagnostic pour les identifier et mettre en place une thérapeutique appropriée.NANTES-Ecole Nat.Vétérinaire (441092302) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF

Etude clinico-pathologique de l'audition chez un modèle canin de mucopolysaccharidose de type 1

La mucopolysaccharidose de type 1 (MPS 1) provoque chez l'enfant une surdité mixte dont la pathogénie est peu connue. Dans un modèle canin de MPS 1 nous avons 1/ étudié les lésions histopathologiques de l'oreille et des voies nerveuses auditives chez 10 chiens MPS 1, 2/ réalisé un suivi auditif par mesure des potentiels évoqués auditifs (PEA) pendant 4 mois chez 3 chiens MPS 1, et 3/ recherché une corrélation entre les lésions et les éventuelles modifications des PEA. L'oreille des chiens MPS 1 présente une surcharge cellulaire identique à celle rapportée chez l'enfant, validant ce modèle pour l'exploration de la fonction auditive. Les voies nerveuses auditives présentent une atteinte sélective avec une susceptibilité accrue apparente des neurones gabaergiques. L'analyse des PEA montre une surdité progressive principalement rétrocochléaire corrélée aux lésions du tronc cérébral, permettant d'envisager l'utilisation des PEA comme suivi clinique non invasif.NANTES-BU Médecine pharmacie (441092101) / SudocTOULOUSE-EN Vétérinaire (315552301) / SudocSudocFranceF