118 research outputs found
Complete genome sequence of bovine viral diarrhea virus subgenotype 2a strain CN10.2015.821, isolated in Piedmont, Italy
Incubation of canine dermal fibroblasts with serum from dogs with atopic dermatitis activates extracellular matrix signalling and represses oxidative phosphorylation
The aim of this study was to investigate the effects on gene expression in canine fibroblasts after incubation with a medium
enriched with atopic dermatitis canine serum (CAD) compared with healthy canine serum (CTRL) and fetal bovine serum
(FBS). Differential Expression and Pathway analysis (iDEP94) in R package (v0.92) was used to identify differentially
expressed genes (DEGs) with a False Discovery Rate of 0.01. DEGs from fibroblasts incubated with CAD serum were
significantly upregulated and enriched in the extracellular matrix (ECM) and focal adhesion signalling but downregulated
in the oxidative phosphorylation pathway. Genes involved in profibrotic processes, such as TGFB1, INHBA, ERK1/2, and
the downward regulated genes (collagens and integrins), were significantly upregulated after fibroblasts were exposed to
CAD serum. The observed downregulation of genes involved in oxidative phosphorylation suggests metabolic dysregulation
toward a myofibroblast phenotype responsible for fibrosis. No differences were found when comparing CTRL with FBS.
The DEGs identified in fibroblasts incubated with CAD serum suggest activation of signalling pathways involved in gradual
differentiation through a myofibroblast precursors that represent the onset of fibrosis. Molecular and metabolic knowledge
of fibroblast changes can be used to identify biomarkers of the disease and new potential pharmacological targets
Design of multiplexing lateral flow immunoassay for detection and typing of foot-and-mouth disease virus using pan-reactive and serotype-specific monoclonal antibodies: Evidence of a new hook effect
Challenging the Hypothesis of in Utero Microbiota Acquisition in Healthy Canine and Feline Pregnancies at Term: Preliminary Data
Development of molecular and antigenic-based rapid tests for the identification of African swine fever virus in different tissues
Investigation of the “Antigen Hook Effect” in Lateral Flow Sandwich Immunoassay: The Case of Lumpy Skin Disease Virus Detection
Possible human-to-dog transmission of SARS-CoV-2, Italy, 2020
We detected severe acute respiratory syndrome coronavirus 2 in an otherwise healthy poodle living with 4 family members who had coronavirus disease. We observed antibodies in serum samples taken from the dog, indicating seroconversion. Full-length genome sequencing showed that the canine and human viruses were identical, suggesting human-to-animal transmission
Escherichia coli induces apoptosis and proliferation of mammary cells
Mammary cell apoptosis and proliferation were assessed after injection of Escherichia coli into the left mammary quarters of six cows. Bacteriological analysis of foremilk samples revealed coliform infection in the injected quarters of four cows. Milk somatic cell counts increased in these quarters and peaked at 24 h after bacterial injection. Body temperature also increased, peaking at 12 h postinjection, The number of apoptotic cells was significantly higher in the mastitic tissue than in the uninfected control. Expression of Bax and interleukin-1 beta converting enzyme increased in the mastitic tissue at 24 h and 72 h postinfection, whereas Bcl-2 expression decreased at 24 h but did not differ significantly from the control at 72 h postinfection, Induction of matrix metalloproteinase-g, stromelysin-1 and urokinase-type plasminogen activator was also observed in the mastitic tissue. Moreover, cell proliferation increased in the infected tissue, These results demonstrate that Escherichia coli-induced mastitis promotes apoptosis and cell proliferation
Long-term persistence of neutralizing SARS-CoV-2 antibodies in pets
We monitored the severe acute respiratory syndrome coronavirus 2 antibody response in seven dogs and two cats by using two multispecies ELISA tests, plaque reduction neutralisation test and virus neutralization. SARS-CoV-2 neutralizing antibodies in pets persisted up to 10 months since the first positive testing, thus replicating observations in COVID-19 human patients
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