Recovery of CD4⁺ and CD8⁺ T-cell subsets after PBSCT with or without pre-transplant ATG-F.

<p>Circulating immune cells phenotypes were assessed for 31/34, 26/30, 28/29 and 20/25 disease-free survivors in the ATG-F cohort on days 40, 100, 180 and 365 after PBSCT, respectively; and for 6/23, 15/17, 9/15 and 7/9 disease-free survivors in the control cohort on days 40, 100, 180 and 365 after PBSCT, respectively. A-F) Absolute cell counts in the peripheral blood of ATG-F (blue box) and control (white box) patients are shown. Box and whisker plots display the median, 25^th and 75^th percentiles of the distribution (box) and whiskers extend to 5^th and 95^th percentiles. The grey horizontal line and shaded grey area show the median and normal range (from 5^th to 95^th percentile) in 22 age-matched healthy controls. G-H) Relative proportions of naïve and memory subsets among CD4+ (G) and CD8+ (H) T compartments were assessed, according to pre-transplant ATG-F or not. Mean and standard deviation are shown. *<i>p</i> <0.05; ** <i>p</i><0.01.</p

Multivariate analysis of factors impacting immune cell levels after myeloablative PBSCT.

<p>Tested variables included: use of pre-transplant ATG-F, patient’s age, donor’s age, type of donor (related or unrelated), donor/patient HLA-match (HLA-matched or HLA-mismatched), donor/patient CMV serostatus (-/-or all other combinations), CD34+ cell dose (log-transformed), postgrafting immunosuppression (cyclosporine+methotrexate, tacrolimus+methotrexate or cyclosporine/tacrolimus alone) and acute GVHD (if it occurred before the day of immune cell assessment). To minimize chance of spurious associations because of multiple comparisons, p <0.01 was considered significant. Only significant variables are shown.</p><p>Multivariate analysis of factors impacting immune cell levels after myeloablative PBSCT.</p

Circulating recent thymic emigrants after PBSCT with or without pre-transplant ATG-F.

<p>Levels of circulating RTE in the peripheral blood of ATG-F (blue box) and control (white box) patients are shown. A) RTE were assessed by sjTRECs quantification assay for 30/30 and 23/25 disease-free survivors in the ATG-F cohort on days 100 and 365 after PBSCT, respectively; and for 11/17 and 7/9 disease-free survivors in the control cohort on days 100 and 365 after PBSCT, respectively. B) RTE were assessed by flow cytometry for 26/30 and 20/25 disease-free survivors in the ATG-F cohort on days 100 and 365 after PBSCT, respectively; and for 15/17 and 7/9 disease-free survivors in the control cohort on days 100 and 365 after PBSCT, respectively. Box and whisker plots display the median, 25^th and 75^th percentiles of the distribution (box) and whiskers extend to 5^th and 95^th percentiles. The grey horizontal line and shaded grey area show the median and normal range (from 5^th to 95^th percentile) in 22 age-matched healthy controls.</p

Cumulative incidence of infections after PBSCT with or without ATG-F.

<p>Cumulative incidences of bacterial (A), viral (B) and fungal (C) infections in ATG-F (solid line) and control (broken line) patients are shown.</p

Patients characteristics.

<p>CIf indicates cumulative incidence function; NA, not applicable.</p><p>* No statistical test is provided due to small sample size.</p><p>^a classified according to Armand et al. [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0130026#pone.0130026.ref033" target="_blank">33</a>].</p><p>^b Absolute neutrophil counts >0.5 x10⁹/L for at least consecutive 3 days.</p><p>Patients characteristics.</p

Lymphoid cell recovery after PBSCT with or without pre-transplant ATG-F.

<p>Levels of circulating lymphoid cells in the peripheral blood of ATG-F (blue box) and control (white box) patients are shown. Cell phenotypes were assessed for 27/34, 27/33, 27/31, 26/30, 24/30, 26/29 and 19/25 disease-free survivors in the ATG-F cohort on days 40, 60, 80, 100, 120, 180 and 365 after PBSCT, respectively; and for 17/23, 11/19, 10/17, 12/17, 11/17, 8/15 and 5/9 disease-free survivors in the control cohort on days 40, 60, 80, 100, 120, 180 and 365 after PBSCT, respectively. Box and whisker plots display the median, 25^th and 75^thpercentiles of the distribution (box) and whiskers extend to 5^th and 95^th percentiles. The grey horizontal line and shaded grey area show the median and normal range (from 5^th to 95^th percentile) in 22 age-matched healthy controls. *<i>p</i> <0.05; ** <i>p</i><0.01.</p