7 research outputs found

    Dataset supporting the illustration of the accuracy of quantification using the Array-DASH process (Figure 9.zip)

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    This data set represents the estimation of the amount of the LSCAN 1 target in the mixtures based on both the peak height and peak area of the C allele signal in the Array-DASH profile from positions 8 and 25. See text file in the package for a further explanation.Please note the addition of a figure to the manuscript has caused the figure number to be increased by 1 compared to those in the file name. </div

    Dataset supporting the illustration of the semi-quantitive nature of the Array-DASH approach (Figure 8.zip)

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    This data set contains ArrayDASH data from mixtures of synthetic oligonucleotides that differ at two specific locations such that one carries T bases and the other carries G bases resulting in an AC genotype call. The oligonucleotide carrying the G base is gradually decreased in the mixtures across the set to construct a standard curve to test the sensitivity of the system for this base combination to show the potential of the system.Please note the addition of a figure to the manuscript has caused the figure number to be increased by 1 compared to those in the file name. </div

    Dataset supporting the illustration of the effect of a heterozygous position on the Array-DASH profiles of adjacent bases (Figure 6.zip).

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    This data set contains ArrayDASH data from mixtures of synthetic oligonucleotides that differ at two specific locations such that one carries T bases and the other carries G bases resulting in an AC genotype call. This is used to investigate the effect of a heterozygous position on the Array-DASH profiles from adjacent positions.Please note the addition of a figure to the manuscript has caused the figure number to be increased by 1 compared to those in the file name. </div

    Data set supporting the illustration of basic base calling using the Array-DASH process. (Figure 2.zip)

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    This dataset relates to the first in a series of a proof of principal for the ArrayDASH process. It shows the results from a test oligo nucleotide sequence (LSCAN 1) that was the perfect reverse compliment of the reference sequence used to design the array. Please see included text file for more information

    Dataset supporting the illustration of the effect of a non-reference base position on adjacent bases calls, in Array-DASH based re-sequencing (Figure 4.zip)

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    This is a dataset relating to the use of the Array-DASH method to two test oligonucleotide sequences that differed at 3 known positions. LSCAN 1 was the perfect reverse compliment of the reference sequence used to design the array while LSCAN 2 contained 3 known changes. See text files for more details.Please note the addition of a figure to the manuscript has caused the figure number to be increased by 1 compared to those in the file name. </div

    Dataset supporting the illustration of the effect of a heterozygous position on an Array-DASH profile (Figure 5.zip)

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    This data set consists of data obtained via the ArrayDASH from 6 mixtures of synthetic oligonucleotide sequences, which were the perfect reverse compliment to the reference sequence used to design the array except at 3 positions where the base was substituted to allow the constriction of mixtures that could represent all 6 possible di-allelic genotypes possible in a normal diploid genomePlease note the addition of a figure to the manuscript has caused the figure number to be increased by 1 compared to those in the file name. </div

    Dataset supporting the re-sequencing of a HIV clone and a patient sample via Array-DASH, and the comparison of the results to those obtained from NGS (Figure 11.zip)

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    This data set contains ArrayDASH results obtained from the Protease and reverse transcriptase genes amplified from a control clone of HIV and a HIV patient.Please note the addition of a figure to the manuscript has caused the figure number to be increased by 1 compared to those in the file name. </div
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