46 research outputs found
G Matrix for Tetrahedral X4 Y 4 Molecules
Since the development of \u27 the so called G F matrix method1 force constants; calculations for many molecules of different structure have be.en performed. It is not difficult to obtain the elements of the G matrix for a molecule directly from s vectors, or from the general tables of g matrix elements?. However, the final G ;k elemoots are Teipo,rted ror most moleCUilar stvuctures. To complete ithe materlial we fist the Gik for tetrahedral X 4 Y 4 , one -0f a few simplestructuir. es .so far not !being discU!Ssed. Thi:s S;tructure is reahzed, e.g. in boron tetrachloride
A monoclonal antibody to the rat Crry/p65 antigen, a complement regulatory membrane protein, stimulates adhesion and proliferation of thymocytes
A murine monoclonal antibody (mAb), 3F10, was produced by fusion of spleen cells obtained from mice immunized with a rat cortical thymic epithelial cell line (R-TNC.1) stimulated with interferon-gamma and P3X myeloma cells. 3F10 recognized an antigen expressed both on thymocytes and non-lymphoid cells in the thymus. Flow cytometry showed that 3F10 stained more than 98% thymocytes and 90% R-TNC.1 cells. Immunoprecipitation and Western blot studies demonstrated that 3F10 reacted with molecules of 55000 and 65000 MW from both thymocyte and R-TNC.1 cell lysates. 3F10 recognized the same antigen on Chinese hamster ovary cells transfected with rat Crry as did 5I2 mAb, confirming the specificity of 3F10 mAb for the rat homologue of mouse Crry/p65, a membrane-bound complement regulatory protein. 3F10 mAb induced homotypic aggregation of thymocytes and exhibited an additive effect on the aggregation evoked by phorbol myristate acetate. The aggregation was dependent on active cell metabolism, intact cytoskeleton, divalent cations and activation of protein phosphatases 1 and 2A (as assessed by use of okadaic acid). In contrast, H-7, HA1004 and genistein partially inhibited, whereas staurosporine potentiated the aggregation of thymocytes triggered by 3F10. 3F10 mAb also stimulated binding of thymocytes to the R-TNC.1 line. Both homotypic and heterotypic adhesive interactions are mediated by leucocyte function-associated antigen-1 (LFA-1). In addition, 3F10 stimulated proliferation of thymocytes induced by suboptimal concentrations of concanavalin A. These data suggest that rat Crry/p65 might be involved in the regulation of both cell adhesion and activation of thymocytes. This is a novel, non-complement-dependent function of Crry/p65
Properties of the Strange Axial Mesons in the Relativized Quark Model
We studied properties of the strange axial mesons in the relativized quark
model. We calculated the decay constant in the quark model and showed how
it can be used to extract the mixing angle
() from the weak decay . The ratio is the most sensitive
measurement and also the most reliable since the largest of the theoretical
uncertainties factor out. However the current bounds extracted from the
TPC/Two-Gamma collaboration measurements are rather weak: we typically obtain
at 68\% C.L. We also calculated the
strong OZI-allowed decays in the pseudoscalar emission model and the flux-tube
breaking model and extracted a mixing angle of . Our analysis also indicates that the heavy quark limit does not give a
good description of the strange mesons.Comment: Revised version to be published in Phys. Rev. D. Minor changes. Latex
file uses revtex version 3 and epsfig, 4 postcript figures are attached. The
full postcript version with embedded figures is available at
ftp://ftp.physics.carleton.ca/pub/theory/godfrey/ocipc9512.ps.
Optimization of a free separation of 30 free amino acids and peptides by capillary zone electrophoresis with indirect absorbance detection: a potential for quantification in physiological fluids
This report describes a rapid, single-run procedure, based on the optimization of capillary electrophoresis (CE) and indirect absorbance detection capabilities, which was developed for the separation and quantification of 30 underivatized physiological amino acids and peptides, usually present in biological fluids. p-Aminosalicylic acid buffered with sodium carbonate at pH 10.2 +/- 0.1 was used as the running electrolyte. Electrophoresis, carried out in a capillary (87 cmx75 mum) at 15 kV potential (normal polarity), separated the examined compounds within 30 min. Limits of detection ranged from 1.93 to 20.08 mumol/l (median 6.71 mumol/l). The method was linear within the 50-200 mumol/l concentration range (r ranged from 0.684 to 0.989, median r=0.934). Within run migration times precision was good (median C.V.=0.7%). Less favorable within run peak area precision (median C.V.=6.6%) was obtained. The analytical procedure presented was successfully tested for separation and quantification of amino acids in physiological fluids, such as plasma or supernatant of macrophage cultures. Sample preparations require only a protein precipitation and dilution step