56 research outputs found
Compușii fenolici în reziduurile de Lavandula angustifolia Mill.
Catedra de farmacognozie și botanică farmaceuticăBackground. Lavandula angustifolia Mill. is a species of the family Lamiaceae, native to the
Mediterranean Basin, which shows antibacterial, antifungal, carminative, sedative and antidepressant
effects, based on acyclic monoterpenoids: (linalool (30-35%), linalyl acetate (10-14.2%), lavandulol,
geraniol) and polyphenol content. Objective of the study. Determination of total polyphenol
compounds and total flavonoids in 2 residues: Lavandulae R-1 (average particle size of 0.5 mm) and
Lavandulae R-2 (average particle size of 15-20 mm). Material and methods. The dry residue from the
aerial parts of Lavender, obtained after extraction of the essential oil by hydrodistillation, was divided
into 2 batches and subjected to the extraction of phenolic compounds with 96% ethanol in the Soxhlet
apparatus, in 5 cycles. The total content of polyphenols was determined spectrophotometrically after
Folin-Ciocalteu, and the total flavonoids were dosed with 25 g/L aluminum chloride solution. Results.
The L. angustifolia species provides significant products to the pharmaceutical and cosmetic industry,
but and lavender residues have not been used so far, after the extraction of volatile oil, although they
contain various chemical compounds. The results show that the Lavandulae R-1 residue, obtained from
a vegetal products with smaller dimensions has a higher total content of polyphenols, expressed in gallic
acid (2.19%) and total flavonoids expressed in rutoside (2, 03%) , followed by Lavandulae R-2 residue,
with a total content of polyphenols (1.12%) and flavonoids (1.43%). Conclusion. The results confirm
that the residues of Lavander can be used as a source of polyphenols and total polyphenol content and
total flavonoids, depend on the method of extraction and the degree of crushing of the vegetal products.Introducere. Lavandula angustifolia Mill. este o specie din familia Lamiaceae, originară din Bazinul
Mediteranean ce manifestă efecte antibacteriene, antifungice, carminative, sedative și antidepresive, în
baza monoterpenoidelor aciclice: (linalool (30-35%), acetat de linalil (10-14.2%), lavandulol, geraniol)
și conținut de polifenoli. Scopul lucrării. Determinarea totalului de polifenoli și de flavonoide în 2
reziduuri: Lavandulae R-1 (dimensiuni medii a particulelor de 0,5 mm) și Lavandulae R-2 (dimensiuni
medii a particulelor de 15-20 mm). Material și Metode. Reziduul uscat din părți aeriene de levănțică,
obținut după extragerea uleiului volatil prin hidrodistilare, a fost împărțit în 2 loturi și supus extragerii
compușilor fenolici cu etanol 96% în aparatul Soxhlet, în 5 cicluri. Conținutul total de polifenoli a fost
determinat spectrofotometric după Folin-Ciocalteu, iar totalul de flavonoide s-a dozat cu soluție de
clorură de aluminiu 25 g/L. Rezultate. Specia L. angistifolia furnizează produse semnificative industriei
farmaceutice și cosmetice, iar reziduurilor de levănțică, după extragerea uleiului volatil, n-au fost utiliate
până în prezent, deși conțin diferiți compuși chimici. Rezultatele denotă, că reziduul Lavandulae R-1,
obținut din produs vegetal cu dimensiuni mai mici are un conținut total mai mare de polifenoli, exprimat
în acid galic (2,19 %) și totalul de flavonoide exprimat în rutozidă (2, 03%), urmat de reziduu
Lavandulae R-2, cu un conținut total de polifenoli (1,12 %) și flavonoide (1,43%). Concluzii.
Rezultatele confirmă faptul că reziduurile de levănțică pot fi utilizate ca sursă de polifenoli, iar
conținutul total de polifenoli și flavonoide depinde de metoda de extragere și de gradul de fărămițare a
produselor vegetale
Compuși fenolici în reziduuri de Lavandula angustifolia Mill.
Catedra de farmacognozie și botanică farmaceutică,
USMF “Nicolae Testemițanu”Introducere. USMF “Nicolae Testemițanu”
Lavandula angustifolia Mill., f. Lamiaceae, originară
din Bazinul Mediteranean, manifestă efecte
antibacteriene, antifungice, carminative, sedative și
antidepresive, în baza monoterpenoidelor aciclice:
(linalool (35%), acetat de linalil (14.%), lavandulol,
geraniol) și conținut de polifenoli.
Scopul studiului.
Determinarea totalului de polifenoli și de flavonoide
în 2 reziduuri: Lavandulae R-1 (dimensiuni medii a
particulelor de 0,5 mm) și Lavandulae R-2
(dimensiuni medii a particulelor de 15-20 mm).
Material și Metode.
Reziduul uscat din părți aeriene de levănțică, obținut
după extragerea UV prin hidrodistilare, divizat în 2
loturi, a fost supus extragerii compușilor fenolici cu
etanol 96% în aparatul Soxhlet, în 5 cicluri.
Conținutul total de polifenoli a fost determinat
spectrofotometric după Folin-Ciocalteu, iar totalul
de flavonoide s-a dozat cu soluție de clorură de
aluminiu 25 g/L.
Rezultate.
Rezultatele denotă, că reziduul Lavandulae (R-1),
obținut din PV cu dimensiuni mai mici (0,5 mm) are
un conținut mai mare de polifenoli, exprimat în acid
galic (2,19 %) și totalul de flavonoide exprimat în
rutozidă (2,03%), urmat de reziduu Lavandulae R-2
(dimensiunile PV 20 mm) cu un conținut de polifenoli
(1,12%) și flavonoide (1,43%).
Concluzii. Reziduurile de levănțică pot fi utilizate ca
sursă de polifenoli, iar conținutul total de polifenoli
și flavonoide depinde de metoda de extragere și de
gradul de fărămițare a produselor vegetale
The weight of vegetal products and phyitodrugs in the List of Autorized Products of Republic of Moldova
Catedra Farmacognozie şi Botanică Farmaceutică, USMF „Nicolae Testemişanu”
Agenţia MedicamentuluiThis study shows that the weight of vegetal products are 15,51%, medicinal species are
3,47%, phytodrugs of single-species are 23,38% and phytodrugs of combinations are 57,64%,
according to the List of Authorized Products of Republic of Moldova.
To mention the necessity of this drugs, we have to extend the range of the phytodrugs to
the List of Autorized Products on the basis of local productions.
S-a realizat un studiu mai profund, prin analiza produselor vegetale, speciilor medicinale
şi fitopreparatelor, cota parte a produselor menţionate în Nomenclatorul de Stat al
Medicamentelor, cât şi clasificarea lor conform indicelui farmacoterapeutic şi conţinutului de
principii active. S-a evaluat prezentarea topului plantelor medicinale utilizate mai frecvent, cât şi
a efectelor farmacoterapeutice
Vegetal products, medicinal species and phytodrugs fabricated in Republic of Moldova
Catedra Farmacognozie şi Botanică Farmaceutică, USMF „Nicolae Testemiţanu”
Centrul Ştiinţific în Domeniul MedicamentuluiThis study shows that the weight of vegetal products fabricated in Republic of Moldova are
75 or 6,48%, medicinal species are 10 or 0,88%, phytodrugs are 153 or 13,23%, 918 (79,41%)
are industrial drugs, according to the List of Authorized Products of Republic of Moldova.
To mention the necessity to passing from quality control system to the quality assurance
system in result of implementation of Good Manufacturing Practice (GMP) in Republic of
Moldova, including fabrication of vegetable products, medicinal species and phytodrugs and
the necessity to extend the range of the phytodrugs to the List of Authorized Products on the
basis of local productions.
S-a realizat un studiu al produselor vegetale (PV), speciilor medicinale (SM) şi
fitopreparatelor (FT) fabricate în Republica Moldova, cota parte a produselor menţionate în
Nomenclatorul de Stat al Medicamentelor, cât şi clasificarea lor conform indicelui
farmacoterapeutic şi conţinutului de principii active. Rezultatele denotă că cota parte a
fitopreparatelor fabricate în ţară constituie 153 sau 13,23%, produselor vegetale în număr de 75
sau 6,48%, iar a speciilor medicinale - 10 produse sau 0,88%, raportate la numărul total de
medicamente fabricate în Republica Moldova.
Totodată, se menţionează necesitatea aprobării şi implementării Regulilor de Bună Practică
de Fabricaţie (GMP) în Republica Moldova, inclusiv pentru fabricarea produselor vegetale,
speciilor medicinale şi fitopreparatelor
Simulation of a Pneumatically Driven Robotic Gripper Used for Handling Cylindrical Workpieces
The paper presents the geometrical model of a robotic gripper with 3
fingers, accomplished by using SolidWorks software, used for grasping
cylindrical workpieces of the same length, but with different diameters.
Applying an action force to the gripper’s pneumatic cylinder piston, for
a certain cylindrical workpiece, the contact forces between the gripper
and the workpiece are determined in the SolidWorks Motion module.
Also, in the same module, a study by finite element method is
accomplished in order to obtain the highest value of von Mises stress
that occurs within the gripper’s finger. This value is compared to the
yield strength value of finger’s material, for the verification of its
structural integrity in operation
Histopatological evaluation extracts of agrimoniae herba and cichorii herba in experimental induced hepatotoxicity
Background. Agrimonia eupatoria L. (Rosaceae) and Cichorium intybus L. (Asteraceae) are
medicinal plants with a long tradition of use for a variety of therapeutic purposes: antiinflammatory, astingent, antioxidant, being rich in phenolic compounds (flavonoids, phenolic acids
and tannins). There is limited data available on their hepatoprotective effects, particularly in relation
to extracts obtained from their aerial parts.
Material ant methods. The plant products derived from the two species were collected throughout
the flowering period and dried extracts of the aerial parts were obtained with 60% (w/w) ethanol.
Toxic hepatitis was induced an in vivo model with CCl4 (0,4 g/100 g) in rats and the protective
effects of the two extracts, in doses of 100, 200, 400 mg of (Agrimoniae herba and Cichorii herba)
were evaluated by biochemical and histological investigations. For histological analysis of the
samples, hematoxylin-euzine staining was performed and the samples were visualized by light
microscopy (tipe Olympus).
Results. The histological analysis (H-Ex90) of the samples determined that in the liver we have
swelling of the hepatocyte cytoplasm with granular inclusions, dilated centrilobular vein, dilated
dysse spaces and interstitial oedema in all groups treated with CCl4; for the maximum dose of
Agrimonia herba extracts (400 mg)- cytoplasmic swelling of hepatocytes with granular inclusions,
dilated centrilobular vein, dilated dysse spaces, interstitial oedema and perilobular vesicular and
hydropic dystrophy were observed, indicating an aggressive process on the liver parenchyma.
Conclusions. The Cichorii herba extract in doses: 100, 200, 400 mg and Agrimoniae herba extract
(100, 200 mg) can be used in further studies for the development of new pharmaceutical forms
Clinical case of relapse in Plasmodium falciparum malaria
Introducere. Malaria rămâne cea mai răspândită maladie
parazitară. Cazurile raportate în republică sunt în rezultatul
călătoriilor în zonele endemice. Malaria tropică tratată eficient cu o combinație pe bază de Artemisinină poate provoca
recrudescență, un nou episod detectabil de parazitemie în
stadiul sanguin. Scopul lucrării. Prezentarea unui caz clinic
la un pacient tratat cu Artesun de recidivă a malariei cu Pl.
falciparum, peste 3 săptămâni, asociată cu trombocitopenie
secundară. Material și metode. Datele au fost prelevate din
fișa medicală a pacientului internat în SCBI Toma Ciorbă,
confirmat parazitologic, prin depistarea agentului patogen
în picătura groasă și frotiul de sânge. Rezultate. Bărbat 29
ani, internat cu accese malarice zilnice timp de 3 zile, asociate cu stare de rău, oboseală, mialgii. S-a întors din Africa cu
11 zile înainte de internare, aflându-se timp de 3 săptămâni,
chimioprofilaxie antimalarică nu a administrat. A fost confirmat prin microscopie cu Pl. falciparum – trofozoiți inelari
4+, tratat cu Artesun în asociere cu Doxiciclina. Externat în
stare satisfăcătoare, cu 3 rezultate negative la Pl. falciparum.
Se internează repetat peste 3 săpt., cu febră 39.0C timp de
3 zile, frisoane, transpirații, vome repetate, diaree, pierdere
ponderală. De la ultima manifestare a malariei nu a călătorit
în afara țării. Confirmat prin microscopie Pl. falciparum-trofozoiți inelari 4+, parazitemie 12365/1mkl sânge. Tratat cu
Cuartem 3 zile, externat cu 3 rezultate negative la malarie.
Concluzie. Recidiva de infecție a fost cauzată de prezența Pl.
în eritrocite dintr-o infecție anterioară cu parazitemie nedetectabilă microscopic. Infecția cu Pl. falciparum nu trebuie
exclusă la pacienții cu simptome de malarie, chiar și după
tratament.Introduction. Malaria remains the most widespread parasitic disease. Cases reported in the republic are the result of
travel to endemic areas. Tropical malaria treated effectively
with an artemisinin can cause recrudescence, a new detectable episode of parasitemia in blood stages. Objective. To
present a clinical case of a patient treated with Artesun for
relapse of malaria with Pl. falciparum, over 3 weeks, associated with secondary thrombocytopenia. Material and
methods. Data were taken from the medical record of the
patient admitted to Toma Ciorbă CHID, confirmed parasitologically, by detection of the pathogen in the thick drop
and blood smear. Results. Male 29 years old, admitted with
daily malarial attacks for 3 days, associated with malaise, fatigue, myalgia. Returned from Africa 11 days before admission, staying for 3 weeks, antimalarial chemoprophylaxis
not administrated. It was confirmed by microscopy with Pl.
falciparum, ring form trophozoides 4+, treated with Artesun
in combination with Doxycycline. Discharged in satisfactory
condition, with 3 negative results for Pl. falciparum. Repeated admission over 3 wks with fever 39.0C for 3 days, chills,
sweats, repeated vomiting, diarrhea, weight loss. Since the
last malaria outbreak he has not travelled outside the country. Confirmed by microscopy Pl. falciparum, ring form trophozoites 4+, parasitaemia 12365/1mkl blood. Treated with
Cuartem 3 days, discharged with 3 negative malaria results.
Conclusion. Relapse of infection was caused by presence of
Pl. in erythrocytes from a previous infection with microscopically undetectable parasitemia. Infection with Pl. falciparum should not be excluded in patients with symptoms of
malaria, even after treatment
Acute toxicity studies of extracts from Lavandula angustifolia mill
Introduction. Fine lavender (Lavandula angustifolia Mill., LA) is a valuable plant, broadly
cultivated for essencial oil production. LA extracts display a broad spectrum of pharmacological
activities, including antioxidant, sedative, anti-inflammatory and antimicrobial properties. The use of
LA extracts as food additives requires a thorough investigation of their toxicity profile. We report in
the current comunication preclinical acute toxicity studies of a LA ethanolic extract.
Material și methods. Wistar rats (n=42) of both sexes, with a body weight of 200 - 400 g, aged
approximately 3 months, were used for the acute toxicity study. Acute toxicity was modeled by
intragastric administration of LA extract solution by gavage to outbred rats. The LA extract was
administered in doses of: control group, group I - 500 mg/kg; II - 1000 mg/kg; III - 1500 mg/kg;
IV - 3000 mg/kg; V - 4000mg/kg; VI – 5000 mg/kg. After 14 days, the animals were euthanized
and blood was collected with the study of biochemical indices (total protein, albumin, urea,
creatinine, AST, ALT).
Results. Acute toxicity studies demonstrated that after internal administration of LA extract to
rats in doses of 500 mg/kg, 1000 mg/kg; 1500 mg/kg, 3000 mg/kg, 4000 mg/kg, 5000 mg/kg no
death of animals. LA extract was established as a relatively harmless biologically active
substance (LD50 being >5000 mg/kg). the harmlessness of the LA extract was confirmed by the
study of the peripheral blood picture in the animals of the group treated with the LA extract with
the administrable dose of 500 mg/kg and 5000 mg/kg in which the level of biochemical indices
did not change.
Conclusion. LA extracts in doses of 500 mg/kg - 5000 mg/kg do not produce changes in
biochemical parameters and can be considered harmless.
Acknowledgements: This work has been funded by ANCD, project “New products with
preventive and therapeutic potential basing on natural products of vegetal origin and modern
methods of organic synthesis”, code 20.80009.8007.03
CURRENT ASPECTS OF ENVIRONMENTAL REGULATIONS AND INTERVENTIONS IN EUROPE - RESEARCH FOCUS ON HUMAN HEALTH
The article focuses on a synthetic presentation of environmental legislation and policies at national and European level, based on the analysis of government strategies that aim to improve living standards through education starting from an early level, through the implementation of waste management resulting from industrial and domestic activities, through the modernization of the rural environment as a result of the financing of water supply, access programme to alternative energy and specifically gas
Current aspects of environmental regulations and interventions in Europe - research focus on human health
The article focuses on a synthetic presentation of environmental legislation and policies at national and European level, based on the analysis of government strategies that aim to improve living standards through education starting from an early level, through the implementation of waste management resulting from industrial and domestic activities, through the modernization of the rural environment as a result of the financing of water supply, access programme to alternative energy and specifically gas.The article focuses on a synthetic presentation of environmental legislation and policies at national and European level, based on the analysis of government strategies that aim to improve living standards through education starting from an early level, through the implementation of waste management resulting from industrial and domestic activities, through the modernization of the rural environment as a result of the financing of water supply, access programme to alternative energy and specifically gas.The article focuses on a synthetic presentation of environmental legislation and policies at national and European level, based on the analysis of government strategies that aim to improve living standards through education starting from an early level, through the implementation of waste management resulting from industrial and domestic activities, through the modernization of the rural environment as a result of the financing of water supply, access programme to alternative energy and specifically gas
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