Compușii fenolici în reziduurile de Lavandula angustifolia Mill.

Catedra de farmacognozie și botanică farmaceuticăBackground. Lavandula angustifolia Mill. is a species of the family Lamiaceae, native to the Mediterranean Basin, which shows antibacterial, antifungal, carminative, sedative and antidepressant effects, based on acyclic monoterpenoids: (linalool (30-35%), linalyl acetate (10-14.2%), lavandulol, geraniol) and polyphenol content. Objective of the study. Determination of total polyphenol compounds and total flavonoids in 2 residues: Lavandulae R-1 (average particle size of 0.5 mm) and Lavandulae R-2 (average particle size of 15-20 mm). Material and methods. The dry residue from the aerial parts of Lavender, obtained after extraction of the essential oil by hydrodistillation, was divided into 2 batches and subjected to the extraction of phenolic compounds with 96% ethanol in the Soxhlet apparatus, in 5 cycles. The total content of polyphenols was determined spectrophotometrically after Folin-Ciocalteu, and the total flavonoids were dosed with 25 g/L aluminum chloride solution. Results. The L. angustifolia species provides significant products to the pharmaceutical and cosmetic industry, but and lavender residues have not been used so far, after the extraction of volatile oil, although they contain various chemical compounds. The results show that the Lavandulae R-1 residue, obtained from a vegetal products with smaller dimensions has a higher total content of polyphenols, expressed in gallic acid (2.19%) and total flavonoids expressed in rutoside (2, 03%) , followed by Lavandulae R-2 residue, with a total content of polyphenols (1.12%) and flavonoids (1.43%). Conclusion. The results confirm that the residues of Lavander can be used as a source of polyphenols and total polyphenol content and total flavonoids, depend on the method of extraction and the degree of crushing of the vegetal products.Introducere. Lavandula angustifolia Mill. este o specie din familia Lamiaceae, originară din Bazinul Mediteranean ce manifestă efecte antibacteriene, antifungice, carminative, sedative și antidepresive, în baza monoterpenoidelor aciclice: (linalool (30-35%), acetat de linalil (10-14.2%), lavandulol, geraniol) și conținut de polifenoli. Scopul lucrării. Determinarea totalului de polifenoli și de flavonoide în 2 reziduuri: Lavandulae R-1 (dimensiuni medii a particulelor de 0,5 mm) și Lavandulae R-2 (dimensiuni medii a particulelor de 15-20 mm). Material și Metode. Reziduul uscat din părți aeriene de levănțică, obținut după extragerea uleiului volatil prin hidrodistilare, a fost împărțit în 2 loturi și supus extragerii compușilor fenolici cu etanol 96% în aparatul Soxhlet, în 5 cicluri. Conținutul total de polifenoli a fost determinat spectrofotometric după Folin-Ciocalteu, iar totalul de flavonoide s-a dozat cu soluție de clorură de aluminiu 25 g/L. Rezultate. Specia L. angistifolia furnizează produse semnificative industriei farmaceutice și cosmetice, iar reziduurilor de levănțică, după extragerea uleiului volatil, n-au fost utiliate până în prezent, deși conțin diferiți compuși chimici. Rezultatele denotă, că reziduul Lavandulae R-1, obținut din produs vegetal cu dimensiuni mai mici are un conținut total mai mare de polifenoli, exprimat în acid galic (2,19 %) și totalul de flavonoide exprimat în rutozidă (2, 03%), urmat de reziduu Lavandulae R-2, cu un conținut total de polifenoli (1,12 %) și flavonoide (1,43%). Concluzii. Rezultatele confirmă faptul că reziduurile de levănțică pot fi utilizate ca sursă de polifenoli, iar conținutul total de polifenoli și flavonoide depinde de metoda de extragere și de gradul de fărămițare a produselor vegetale

Compuși fenolici în reziduuri de Lavandula angustifolia Mill.

Catedra de farmacognozie și botanică farmaceutică, USMF “Nicolae Testemițanu”Introducere. USMF “Nicolae Testemițanu” Lavandula angustifolia Mill., f. Lamiaceae, originară din Bazinul Mediteranean, manifestă efecte antibacteriene, antifungice, carminative, sedative și antidepresive, în baza monoterpenoidelor aciclice: (linalool (35%), acetat de linalil (14.%), lavandulol, geraniol) și conținut de polifenoli. Scopul studiului. Determinarea totalului de polifenoli și de flavonoide în 2 reziduuri: Lavandulae R-1 (dimensiuni medii a particulelor de 0,5 mm) și Lavandulae R-2 (dimensiuni medii a particulelor de 15-20 mm). Material și Metode. Reziduul uscat din părți aeriene de levănțică, obținut după extragerea UV prin hidrodistilare, divizat în 2 loturi, a fost supus extragerii compușilor fenolici cu etanol 96% în aparatul Soxhlet, în 5 cicluri. Conținutul total de polifenoli a fost determinat spectrofotometric după Folin-Ciocalteu, iar totalul de flavonoide s-a dozat cu soluție de clorură de aluminiu 25 g/L. Rezultate. Rezultatele denotă, că reziduul Lavandulae (R-1), obținut din PV cu dimensiuni mai mici (0,5 mm) are un conținut mai mare de polifenoli, exprimat în acid galic (2,19 %) și totalul de flavonoide exprimat în rutozidă (2,03%), urmat de reziduu Lavandulae R-2 (dimensiunile PV 20 mm) cu un conținut de polifenoli (1,12%) și flavonoide (1,43%). Concluzii. Reziduurile de levănțică pot fi utilizate ca sursă de polifenoli, iar conținutul total de polifenoli și flavonoide depinde de metoda de extragere și de gradul de fărămițare a produselor vegetale

The weight of vegetal products and phyitodrugs in the List of Autorized Products of Republic of Moldova

Catedra Farmacognozie şi Botanică Farmaceutică, USMF „Nicolae Testemişanu” Agenţia MedicamentuluiThis study shows that the weight of vegetal products are 15,51%, medicinal species are 3,47%, phytodrugs of single-species are 23,38% and phytodrugs of combinations are 57,64%, according to the List of Authorized Products of Republic of Moldova. To mention the necessity of this drugs, we have to extend the range of the phytodrugs to the List of Autorized Products on the basis of local productions. S-a realizat un studiu mai profund, prin analiza produselor vegetale, speciilor medicinale şi fitopreparatelor, cota parte a produselor menţionate în Nomenclatorul de Stat al Medicamentelor, cât şi clasificarea lor conform indicelui farmacoterapeutic şi conţinutului de principii active. S-a evaluat prezentarea topului plantelor medicinale utilizate mai frecvent, cât şi a efectelor farmacoterapeutice

Vegetal products, medicinal species and phytodrugs fabricated in Republic of Moldova

Catedra Farmacognozie şi Botanică Farmaceutică, USMF „Nicolae Testemiţanu” Centrul Ştiinţific în Domeniul MedicamentuluiThis study shows that the weight of vegetal products fabricated in Republic of Moldova are 75 or 6,48%, medicinal species are 10 or 0,88%, phytodrugs are 153 or 13,23%, 918 (79,41%) are industrial drugs, according to the List of Authorized Products of Republic of Moldova. To mention the necessity to passing from quality control system to the quality assurance system in result of implementation of Good Manufacturing Practice (GMP) in Republic of Moldova, including fabrication of vegetable products, medicinal species and phytodrugs and the necessity to extend the range of the phytodrugs to the List of Authorized Products on the basis of local productions. S-a realizat un studiu al produselor vegetale (PV), speciilor medicinale (SM) şi fitopreparatelor (FT) fabricate în Republica Moldova, cota parte a produselor menţionate în Nomenclatorul de Stat al Medicamentelor, cât şi clasificarea lor conform indicelui farmacoterapeutic şi conţinutului de principii active. Rezultatele denotă că cota parte a fitopreparatelor fabricate în ţară constituie 153 sau 13,23%, produselor vegetale în număr de 75 sau 6,48%, iar a speciilor medicinale - 10 produse sau 0,88%, raportate la numărul total de medicamente fabricate în Republica Moldova. Totodată, se menţionează necesitatea aprobării şi implementării Regulilor de Bună Practică de Fabricaţie (GMP) în Republica Moldova, inclusiv pentru fabricarea produselor vegetale, speciilor medicinale şi fitopreparatelor

Simulation of a Pneumatically Driven Robotic Gripper Used for Handling Cylindrical Workpieces

The paper presents the geometrical model of a robotic gripper with 3 fingers, accomplished by using SolidWorks software, used for grasping cylindrical workpieces of the same length, but with different diameters. Applying an action force to the gripper’s pneumatic cylinder piston, for a certain cylindrical workpiece, the contact forces between the gripper and the workpiece are determined in the SolidWorks Motion module. Also, in the same module, a study by finite element method is accomplished in order to obtain the highest value of von Mises stress that occurs within the gripper’s finger. This value is compared to the yield strength value of finger’s material, for the verification of its structural integrity in operation

Histopatological evaluation extracts of agrimoniae herba and cichorii herba in experimental induced hepatotoxicity

Background. Agrimonia eupatoria L. (Rosaceae) and Cichorium intybus L. (Asteraceae) are medicinal plants with a long tradition of use for a variety of therapeutic purposes: antiinflammatory, astingent, antioxidant, being rich in phenolic compounds (flavonoids, phenolic acids and tannins). There is limited data available on their hepatoprotective effects, particularly in relation to extracts obtained from their aerial parts. Material ant methods. The plant products derived from the two species were collected throughout the flowering period and dried extracts of the aerial parts were obtained with 60% (w/w) ethanol. Toxic hepatitis was induced an in vivo model with CCl4 (0,4 g/100 g) in rats and the protective effects of the two extracts, in doses of 100, 200, 400 mg of (Agrimoniae herba and Cichorii herba) were evaluated by biochemical and histological investigations. For histological analysis of the samples, hematoxylin-euzine staining was performed and the samples were visualized by light microscopy (tipe Olympus). Results. The histological analysis (H-Ex90) of the samples determined that in the liver we have swelling of the hepatocyte cytoplasm with granular inclusions, dilated centrilobular vein, dilated dysse spaces and interstitial oedema in all groups treated with CCl4; for the maximum dose of Agrimonia herba extracts (400 mg)- cytoplasmic swelling of hepatocytes with granular inclusions, dilated centrilobular vein, dilated dysse spaces, interstitial oedema and perilobular vesicular and hydropic dystrophy were observed, indicating an aggressive process on the liver parenchyma. Conclusions. The Cichorii herba extract in doses: 100, 200, 400 mg and Agrimoniae herba extract (100, 200 mg) can be used in further studies for the development of new pharmaceutical forms

Clinical case of relapse in Plasmodium falciparum malaria

Introducere. Malaria rămâne cea mai răspândită maladie parazitară. Cazurile raportate în republică sunt în rezultatul călătoriilor în zonele endemice. Malaria tropică tratată eficient cu o combinație pe bază de Artemisinină poate provoca recrudescență, un nou episod detectabil de parazitemie în stadiul sanguin. Scopul lucrării. Prezentarea unui caz clinic la un pacient tratat cu Artesun de recidivă a malariei cu Pl. falciparum, peste 3 săptămâni, asociată cu trombocitopenie secundară. Material și metode. Datele au fost prelevate din fișa medicală a pacientului internat în SCBI Toma Ciorbă, confirmat parazitologic, prin depistarea agentului patogen în picătura groasă și frotiul de sânge. Rezultate. Bărbat 29 ani, internat cu accese malarice zilnice timp de 3 zile, asociate cu stare de rău, oboseală, mialgii. S-a întors din Africa cu 11 zile înainte de internare, aflându-se timp de 3 săptămâni, chimioprofilaxie antimalarică nu a administrat. A fost confirmat prin microscopie cu Pl. falciparum – trofozoiți inelari 4+, tratat cu Artesun în asociere cu Doxiciclina. Externat în stare satisfăcătoare, cu 3 rezultate negative la Pl. falciparum. Se internează repetat peste 3 săpt., cu febră 39.0C timp de 3 zile, frisoane, transpirații, vome repetate, diaree, pierdere ponderală. De la ultima manifestare a malariei nu a călătorit în afara țării. Confirmat prin microscopie Pl. falciparum-trofozoiți inelari 4+, parazitemie 12365/1mkl sânge. Tratat cu Cuartem 3 zile, externat cu 3 rezultate negative la malarie. Concluzie. Recidiva de infecție a fost cauzată de prezența Pl. în eritrocite dintr-o infecție anterioară cu parazitemie nedetectabilă microscopic. Infecția cu Pl. falciparum nu trebuie exclusă la pacienții cu simptome de malarie, chiar și după tratament.Introduction. Malaria remains the most widespread parasitic disease. Cases reported in the republic are the result of travel to endemic areas. Tropical malaria treated effectively with an artemisinin can cause recrudescence, a new detectable episode of parasitemia in blood stages. Objective. To present a clinical case of a patient treated with Artesun for relapse of malaria with Pl. falciparum, over 3 weeks, associated with secondary thrombocytopenia. Material and methods. Data were taken from the medical record of the patient admitted to Toma Ciorbă CHID, confirmed parasitologically, by detection of the pathogen in the thick drop and blood smear. Results. Male 29 years old, admitted with daily malarial attacks for 3 days, associated with malaise, fatigue, myalgia. Returned from Africa 11 days before admission, staying for 3 weeks, antimalarial chemoprophylaxis not administrated. It was confirmed by microscopy with Pl. falciparum, ring form trophozoides 4+, treated with Artesun in combination with Doxycycline. Discharged in satisfactory condition, with 3 negative results for Pl. falciparum. Repeated admission over 3 wks with fever 39.0C for 3 days, chills, sweats, repeated vomiting, diarrhea, weight loss. Since the last malaria outbreak he has not travelled outside the country. Confirmed by microscopy Pl. falciparum, ring form trophozoites 4+, parasitaemia 12365/1mkl blood. Treated with Cuartem 3 days, discharged with 3 negative malaria results. Conclusion. Relapse of infection was caused by presence of Pl. in erythrocytes from a previous infection with microscopically undetectable parasitemia. Infection with Pl. falciparum should not be excluded in patients with symptoms of malaria, even after treatment

Acute toxicity studies of extracts from Lavandula angustifolia mill

Introduction. Fine lavender (Lavandula angustifolia Mill., LA) is a valuable plant, broadly cultivated for essencial oil production. LA extracts display a broad spectrum of pharmacological activities, including antioxidant, sedative, anti-inflammatory and antimicrobial properties. The use of LA extracts as food additives requires a thorough investigation of their toxicity profile. We report in the current comunication preclinical acute toxicity studies of a LA ethanolic extract. Material și methods. Wistar rats (n=42) of both sexes, with a body weight of 200 - 400 g, aged approximately 3 months, were used for the acute toxicity study. Acute toxicity was modeled by intragastric administration of LA extract solution by gavage to outbred rats. The LA extract was administered in doses of: control group, group I - 500 mg/kg; II - 1000 mg/kg; III - 1500 mg/kg; IV - 3000 mg/kg; V - 4000mg/kg; VI – 5000 mg/kg. After 14 days, the animals were euthanized and blood was collected with the study of biochemical indices (total protein, albumin, urea, creatinine, AST, ALT). Results. Acute toxicity studies demonstrated that after internal administration of LA extract to rats in doses of 500 mg/kg, 1000 mg/kg; 1500 mg/kg, 3000 mg/kg, 4000 mg/kg, 5000 mg/kg no death of animals. LA extract was established as a relatively harmless biologically active substance (LD50 being >5000 mg/kg). the harmlessness of the LA extract was confirmed by the study of the peripheral blood picture in the animals of the group treated with the LA extract with the administrable dose of 500 mg/kg and 5000 mg/kg in which the level of biochemical indices did not change. Conclusion. LA extracts in doses of 500 mg/kg - 5000 mg/kg do not produce changes in biochemical parameters and can be considered harmless. Acknowledgements: This work has been funded by ANCD, project “New products with preventive and therapeutic potential basing on natural products of vegetal origin and modern methods of organic synthesis”, code 20.80009.8007.03

CURRENT ASPECTS OF ENVIRONMENTAL REGULATIONS AND INTERVENTIONS IN EUROPE - RESEARCH FOCUS ON HUMAN HEALTH

The article focuses on a synthetic presentation of environmental legislation and policies at national and European level, based on the analysis of government strategies that aim to improve living standards through education starting from an early level, through the implementation of waste management resulting from industrial and domestic activities, through the modernization of the rural environment as a result of the financing of water supply, access programme to alternative energy and specifically gas

Current aspects of environmental regulations and interventions in Europe - research focus on human health

The article focuses on a synthetic presentation of environmental legislation and policies at national and European level, based on the analysis of government strategies that aim to improve living standards through education starting from an early level, through the implementation of waste management resulting from industrial and domestic activities, through the modernization of the rural environment as a result of the financing of water supply, access programme to alternative energy and specifically gas.The article focuses on a synthetic presentation of environmental legislation and policies at national and European level, based on the analysis of government strategies that aim to improve living standards through education starting from an early level, through the implementation of waste management resulting from industrial and domestic activities, through the modernization of the rural environment as a result of the financing of water supply, access programme to alternative energy and specifically gas.The article focuses on a synthetic presentation of environmental legislation and policies at national and European level, based on the analysis of government strategies that aim to improve living standards through education starting from an early level, through the implementation of waste management resulting from industrial and domestic activities, through the modernization of the rural environment as a result of the financing of water supply, access programme to alternative energy and specifically gas