Arguments prove nothing unless verified. A commentary on Kaufman's “Epidemiologic analysis of racial/ethnic disparities: Some fundamental issues and a cautionary example”

http://dx.doi.org/10.1016/j.socscimed.2007.11.04

Identification of Faint Chandra X-ray Sources in the Core-Collapsed Globular Cluster NGC 6752

We have searched for optical identifications for 39 Chandra X-ray sources that lie within the 1.9 arcmin half-mass radius of the nearby (d = 4.0 kpc), core-collapsed globular cluster, NGC 6752, using deep Hubble Space Telescope ACS/WFC imaging in B435, R625, and H alpha. Photometry of these images allows us to classify candidate counterparts based primarily on color-magnitude and color-color diagram location. The color-color diagram is particularly useful for quantifying the H alpha line equivalent width. In addition to recovering 11 previously detected optical counterparts, we propose 20 new optical IDs. In total, there are 16 likely or less certain cataclysmic variables (CVs), nine likely or less certain chromospherically active binaries, three galaxies, and three active galactic nuclei (AGNs). The latter three sources, which had been identified as likely CVs by previous investigations, now appear to be extragalactic objects based on their proper motions. As we previously found for NGC 6397, the CV candidates in NGC 6752 fall into a bright group that is centrally concentrated relative to the turnoff-mass stars and a faint group that has a spatial distribution that is more similar to that of the turnoff-mass stars. This is consistent with an evolutionary scenario in which CVs are produced by dynamical interactions near the cluster center and diffuse to larger radius orbits as they age.Comment: 26 pages, 18 figure

Selective iodination and polypeptide composition of pinocytic vesicles

We describe a method for the specific radioiodination of pinocytic vesicles (PVs) based upon the simultaneous endocytosis of lactoperoxidase (LPO) and glucose oxidase (GO). Initial experiments indicated that LPO was interiorized by the macrophage cell line J774 by fluid phase pinocytosis and without detectable binding to the plasma membrane (PM). Interiorization varied linearly with enzyme concentration and exposure time, was temperature dependent, and was undetectable at 4 °C. Employing EM cytochemistry, LPO activity was restricted to PVs after a 3- to 5-min pulse at 37° C. These results formed the basis of the method for iodinating the luminal surface of PVs: 5-min exposure to both LPO and GO at 37 °C followed by washes and iodination (addition of \u27 25 1 and glucose) at 4°C. Enzyme-dependent incorporation of iodide into the polypeptides of both PV membrane and contents occurred. Several lines of evidence indicated that there was selective labeling of PV as opposed to PM. Iodination did not occur if the pinocytic uptake of LPO and GO was inhibited by low temperature. EM autoradiography showed a cytoplasmic localization of grains, whereas a clear PM association was evident with surface labeling. LPO was iodinated only after PV labeling and was present within organelles demonstrating latency. After PV iodination, \u3e75% of several labeled membrane antigens could be immunoprecipitated by monoclonal antibodies only after cell lysis. In contrast, all labeled antigens were accessible to antibody on intact cells after surface labeling. The polypep tide compositions of PM and PV membrane were compared by SDS polyacrylamide gel electrophoresis and by quantitative immune precipitation using a panel of anti-1774 monoclonal antibodies. The electrophoretic profiles of iodinated proteins (15-20 bands) were strikingly similar in NP-40 lysates of both PV and PM iodinated cells. In addition, eight membrane antigens examined by immune precipitation, including the trypsin-resistant immunoglobulin (Fc) receptor and the H-2Dd histocompatibility antigen, were found to be iodinated to the same relative extents by both labeling procedures. We conclude that PV membrane is formed from a representative sample of PM polypeptide components

Identification Of Faint Chandra X-Ray Sources In The Core-Collapsed Globular Cluster NGC 6397: Evidence For A Bimodal Cataclysmic Variable Population

We have searched for optical identifications for 79 Chandra X-ray sources that lie within the half-mass radius of the nearby, core-collapsed globular cluster NGC 6397, using deep Hubble Space Telescope Advanced Camera for Surveys Wide Field Channel imaging in H alpha, R, and B. Photometry of these images allows us to classify candidate counterparts based on color-magnitude diagram location. In addition to recovering nine previously detected cataclysmic variables (CVs), we have identified six additional faint CV candidates, a total of 42 active binaries (ABs), two millisecond pulsars, one candidate active galactic nucleus, and one candidate interacting galaxy pair. Of the 79 sources, 69 have a plausible optical counterpart. The 15 likely and possible CVs in NGC 6397 mostly fall into two groups: a brighter group of six for which the optical emission is dominated by contributions from the secondary and accretion disk and a fainter group of seven for which the white dwarf dominates the optical emission. There are two possible transitional objects that lie between these groups. The faintest CVs likely lie near the minimum of the CV period distribution, where an accumulation is expected. The spatial distribution of the brighter CVs is much more centrally concentrated than those of the fainter CVs and the ABs. This may represent the result of an evolutionary process in which CVs are produced by dynamical interactions, such as exchange reactions, near the cluster center and are scattered to larger orbital radii, over their lifetimes, as they age and become fainter.NASA HST-GO-10257ANSF REU AST-0452975NSERCCIFARAstronom

Epidemiologic analysis of racial/ethnic disparities: A rejoinder to Frank and Exner

http://dx.doi.org/10.1016/j.socscimed.2007.11.04

Temperature Effects on Threshold Counterion Concentration to Induce Aggregation of fd Virus

We seek to determine the mechanism of like-charge attraction by measuring the temperature dependence of critical divalent counterion concentration ($\rm{C_{c}}$) for the aggregation of fd viruses. We find that an increase in temperature causes $\rm{C_c}$ to decrease, primarily due to a decrease in the dielectric constant ($\epsilon$) of the solvent. At a constant $\epsilon$, $\rm{C_c}$ is found to increase as the temperature increases. The effects of $T$ and $\epsilon$ on $\rm {C_{c}}$ can be combined to that of one parameter: Bjerrum length ($l_{B}$). $\rm{C_{c}}$ decreases exponentially as $l_{B}$ increases, suggesting that entropic effect of counterions plays an important role at the onset of bundle formation.Comment: 12 pages, 3 figure