NOTES: RANGE EXTENSION OF THE VIRGINIA OPOSSUM (DIDELPHIS VIRGINIANA) IN NORTH DAKOTA

The Virginia opossum (Didelphis virginiana) is broadly distributed across North America from Costa Rica in the south to southern Ontario in the north and from the southern Great Plains in the west to the eastern United States. The Virginia opossum also was introduced multiple times to thePacific Coast beginning in the late 1800s and has established populations in that region (Gardner and Sunquist 2003). This species is a habitat generalist known to frequent wetland and hardwood habitats but also can be found in grasslands, along forest edges, and in agricultural and suburban settings throughout its range (Gardner and Sunquist 2003, Beatty et al. 2014). However, the Virginia opossum is adapted poorly to winter, limiting its northern distribution to more tolerable warmer climates. It does not hibernate or exhibit torpor, and it will remain in its den rather than forage on nights when temperatures are below freezing or when there is deep snow, risking starvation if more than 54 days of winter are too harsh to forage (Brocke 1970). Despite these limitations, the Virginia opossum has expanded north in recent decades (Myers et al. 2009) and has been documented in novel areas of the Upper Midwest and New England (e.g., Dice 1927, Goodwin 1935, Jackson 1961). Both climate change and human land use alteration have been identified as contributing factors to their current range expansion. A recent study conducted across Michigan and Wisconsin identified reduced days of snow on the ground and increased agricultural land as two key factors facilitating the opossum’s expansion in the Midwest (Walsh and Tucker 2017). As generalist omnivores, opossums benefit from increased road kill and resources provided by agricultural practices (Beatty et al. 2014). Humans are further ameliorating winter conditions by providing shelter and easily accessible food, as evidenced by opossums in urban areas weighing more than individuals in adjacent natural habitats (Kanda 2005, Wright et al. 2012)

The Mass-Radius Relation Of Young Stars. I. Usco 5, An M4.5 Eclipsing Binary In Upper Scorpius Observed By K2

We present the discovery that UScoCTIO 5, a known spectroscopic binary in the Upper Scorpius star-forming region (P = 34 days, M-tot sin(i) = 0.64M(circle dot)), is an eclipsing system with both primary and secondary eclipses apparent in K2 light curves obtained during Campaign 2. We have simultaneously fit the eclipse profiles from the K2 light curves and the existing RV data to demonstrate that UScoCTIO 5 consists of a pair of nearly identical M4.5 stars with M-A = 0.329 +/- 0.002 M-circle dot, R-A = 0.834 +/- 0.006 R-circle dot, M-B = 0.317 +/- 0.002 M-circle dot, and R-B = 0.810 +/- 0.006 R-circle dot. The radii are broadly consistent with pre-main-sequence ages predicted by stellar evolutionary models, but none agree to within the uncertainties. All models predict systematically incorrect masses at the 25%-50% level for the HR diagram position of these mid-M dwarfs, suggesting significant modifications to mass-dependent outcomes of star and planet formation. The form of the discrepancy for most model sets is not that they predict luminosities that are too low, but rather that they predict temperatures that are too high, suggesting that the models do not fully encompass the physics of energy transport (via convection and/or missing opacities) and/or a miscalibration of the SpT-T-eff scale. The simplest modification to the models (changing T-eff to match observations) would yield an older age for this system, in line with the recently proposed older age of Upper Scorpius (tau similar to 11 Myr).NASA Science Mission directorateW. M. Keck FoundationAstronom

Pilus distribution among lineages of group b <i>streptococcus</i>: an evolutionary and clinical perspective

&lt;b&gt;Background&lt;/b&gt;&lt;p&gt;&lt;/p&gt; Group B Streptococcus (GBS) is an opportunistic pathogen in both humans and bovines. Epidemiological and phylogenetic analyses have found strains belonging to certain phylogenetic lineages to be more frequently associated with invasive newborn disease, asymptomatic maternal colonization, and subclinical bovine mastitis. Pilus structures in GBS facilitate colonization and invasion of host tissues and play a role in biofilm formation, though few large-scale studies have estimated the frequency and diversity of the three pilus islands (PIs) across diverse genotypes. Here, we examined the distribution of pilus islands (PI) 1, 2a and 2b among 295 GBS strains representing 73 multilocus sequence types (STs) belonging to eight clonal complexes. PCR-based RFLP was also used to evaluate variation in the genes encoding pilus backbone proteins of PI-2a and PI-2b.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Results&lt;/b&gt;&lt;p&gt;&lt;/p&gt; All 295 strains harbored one of the PI-2 variants and most human-derived strains contained PI-1. Bovine-derived strains lacked PI-1 and possessed a unique PI-2b backbone protein allele. Neonatal strains more frequently had PI-1 and a PI-2 variant than maternal colonizing strains, and most CC-17 strains had PI-1 and PI-2b with a distinct backbone protein allele. Furthermore, we present evidence for the frequent gain and loss of genes encoding certain pilus types.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Conclusions&lt;/b&gt;&lt;p&gt;&lt;/p&gt; These data suggest that pilus combinations impact host specificity and disease presentation and that diversification often involves the loss or acquisition of PIs. Such findings have implications for the development of GBS vaccines that target the three pilus islands

Integrated orbital servicing study for low-cost payload programs. Volume 2: Technical and cost analysis

Orbital maintenance concepts were examined in an effort to determine a cost effective orbital maintenance system compatible with the space transportation system. An on-orbit servicer maintenance system is recommended as the most cost effective system. A pivoting arm on-orbit servicer was selected and a preliminary design was prepared. It is indicated that orbital maintenance does not have any significant impact on the space transportation system

Optimal search strategies for hidden targets

What is the fastest way of finding a randomly hidden target? This question of general relevance is of vital importance for foraging animals. Experimental observations reveal that the search behaviour of foragers is generally intermittent: active search phases randomly alternate with phases of fast ballistic motion. In this letter, we study the efficiency of this type of two states search strategies, by calculating analytically the mean first passage time at the target. We model the perception mecanism involved in the active search phase by a diffusive process. In this framework, we show that the search strategy is optimal when the average duration of "motion phases" varies like the power either 3/5 or 2/3 of the average duration of "search phases", depending on the regime. This scaling accounts for experimental data over a wide range of species, which suggests that the kinetics of search trajectories is a determining factor optimized by foragers and that the perception activity is adequately described by a diffusion process.Comment: 4 pages, 5 figures. to appear in Phys. Rev. Let

Targeted surveillance detected novel beaked whale circovirus in ten new host cetacean species across the Pacific basin

The first cetacean circovirus, beaked whale circovirus (BWCV), was recently reported in a Longman’s beaked whale (Indopacetus pacificus) stranded in Hawai‘i and represents an emergent disease with unknown population impacts. In other species, circovirus infection may cause mortality or opportunistic co-infection by other pathogens. We report on a targeted surveillance of stranded cetaceans in the Pacific basin, including archived beaked whale species, strandings where pathological findings suggested disease presence, mass stranded animals, and additional individuals to represent a broad range of Hawaiian cetacean species. Archived tissues primarily from the brain, kidney, liver, lung, spleen, and lymph nodes of individuals stranded between 2000 and 2020 (n=30) were tested by PCR for the presence of BWCV. Suspect positive tissue amplicons were confirmed as BWCV through sequencing. Of the screened individuals, 15 animals tested positive in one or more tissues, with a single striped dolphin (Stenella coeruleoalba) testing positive in all six tissues. The highest rate of detection among positive cases was found in the brain (69%), followed by lymph (67%) and lung tissues (64%). Additionally, co-infections of cetacean morbillivirus (n=3), Brucella ceti (n=1), and Toxoplasma gondii (n=1) were found among the positive cases. These results expand the potential host range for BWCV into ten additional odontocete species. New host species include a dwarf sperm whale (Kogia sima) that stranded on O‘ahu in 2000, predating the initial case of BWCV. The results broaden the known geographic range of BWCV to Saipan in the Western Pacific, and American Samoa in the South Pacific, where stranded Cuvier’s beaked whales (Ziphius cavirostris) tested positive. Although the clinical significance is currently unknown, this study demonstrates that BWCV has a high prevalence within targeted cetacean screening efforts. Infectious diseases pose a major threat to cetaceans and BWCV may represent an important emerging disease within populations spanning the central, Western, and South Pacific

Estimating oceanic turbulence dissipation from seismic images

Author Posting. © American Meteorological Society, 2013. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Atmospheric and Oceanic Technology 30 (2013): 1767–1788, doi:10.1175/JTECH-D-12-00140.1.Seismic images of oceanic thermohaline finestructure record vertical displacements from internal waves and turbulence over large sections at unprecedented horizontal resolution. Where reflections follow isopycnals, their displacements can be used to estimate levels of turbulence dissipation, by applying the Klymak–Moum slope spectrum method. However, many issues must be considered when using seismic images for estimating turbulence dissipation, especially sources of random and harmonic noise. This study examines the utility of seismic images for estimating turbulence dissipation in the ocean, using synthetic modeling and data from two field surveys, from the South China Sea and the eastern Pacific Ocean, including the first comparison of turbulence estimates from seismic images and from vertical shear. Realistic synthetic models that mimic the spectral characteristics of internal waves and turbulence show that reflector slope spectra accurately reproduce isopycnal slope spectra out to horizontal wavenumbers of 0.04 cpm, corresponding to horizontal wavelengths of 25 m. Using seismic reflector slope spectra requires recognition and suppression of shot-generated harmonic noise and restriction of data to frequency bands with signal-to-noise ratios greater than about 4. Calculation of slope spectra directly from Fourier transforms of the seismic data is necessary to determine the suitability of a particular dataset to turbulence estimation from reflector slope spectra. Turbulence dissipation estimated from seismic reflector displacements compares well to those from 10-m shear determined by coincident expendable current profiler (XCP) data, demonstrating that seismic images can produce reliable estimates of turbulence dissipation in the ocean, provided that random noise is minimal and harmonic noise is removed.This work was funded by NSF Grants 0452744, 0405654, and 0648620, and ONR/DEPSCoR Grant DODONR40027.2014-02-0

Heart Rate Responses to Unaided Orion Side Hatch Egress in the Neutral Buoyancy Laboratory

NASA is developing the Orion capsule as a vehicle for transporting crewmembers to and from the International Space Station (ISS) and for future human space exploration missions. Orion and other commercial vehicles are designed to splash down in the ocean where nominally support personnel will assist crewmembers in egressing the vehicle. However, off-nominal scenarios will require crewmembers to egress the vehicle unaided, deploy survival equipment, and ingress a life raft. PURPOSE: To determine the heart rate (HR) responses to unaided Orion side hatch egress and raft ingress as a part of the NASA Crew Survival Engineering Team's evaluation of the PORT Orion mockup in the Neutral Buoyancy Laboratory (NBL). METHODS: Nineteen test subjects, including four astronauts (N=19, 14 males/5 females, 38.6+/-8.4 y, 174.4+/-9.6 cm, 75.7+/-13.1 kg), completed a graded maximal test on a cycle ergometer to determine VO2peak and HRpeak and were divided into five crews of four members each; one subject served on two crews. Each crew was required to deploy a life raft, egress the Orion vehicle from the side hatch, and ingress the life raft with two 8 kg emergency packs per crew. Each crew performed this activity one to three times; a total of ten full egresses were completed. Subjects wore a suit that was similar in form, mass, and function to the Modified Advanced Crew Escape Suit (MACES) including helmet, gloves, boots, supplemental O2 bottles, and a CO2-inflated life preserver (approx.18 kg); subjects began each trial seated supine in the PORT Orion mockup with seat belts and mockup O2 and communication connections and ended each trial with all four crewmembers inside the life raft. RESULTS: VO2peak was 40.8+/-6.8 mL/kg/min (3.1+/-0.7 L/min); HRpeak was 181+/-10 bpm. Total egress time across trials was 5.0+/-1.6 min (range: 2.8-8.0 min); all subjects were able to successfully complete all trials. Average maximum HR at activity start, at the hatch opening, in the water, and in the raft, was 108, 137, 147, and 153 bpm, respectively; these values corresponded to 59+/-10%, 73+/-8%, 82+/-3%, and 84+/-6% of HRpeak, respectively. The highest HRs were seen after raft ingress and ranged from 72-99% HRpeak. Across all trials, cumulative averages of 5.4, 3.0, 1.1, and 0.2 min were spent at HRs >60%, >70%, >80%, and >90% HRpeak, respectively. CONCLUSION: Unaided Orion side hatch egress in the NBL is a relatively short-duration activity that elicits a high HR response for several min. Although all crewmembers successfully completed this activity, additional factors such as high seas, poor visibility, an incapacitated crewmember, neurovestibular perturbation, and neuromuscular deconditioning characteristic of a true operational environment may increase the physiologic demand (or decrease crewmembers' physiologic capacity) of unaided Orion side hatch egress. Additionally, landing conditions may require the crewmembers to egress from the top hatch, which is expected to be even more physiologically demanding; this condition will be evaluated in subsequent collaborative testing with the NASA Crew Survival Engineering Team