4,600 research outputs found

    The Effects of Low and High Gravity on Neurospora Crassa

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    We studied the effects of different gravity levels on Neurospora crassa and its morphology. This research was conducted by simulating low and high gravity effects in different operating procedures. For the low gravity simulation, the experiment was conducted by using a clinostat to constantly disorient the samples at a very slow rate. For the high gravity simulation, N. crassa was subjected to different amounts of g forces by placing the samples in a centrifuge and subjected the samples to increasing amounts of revolutions per minute. The results of these experiments conclude that in low-gravity situations, the N. crassa showed sparse growth and some cytoplasmic failure. In the high gravity situations, the samples showed that at around 1,000 to 2,000 RPMs, there were no changes in the morphology, but at higher RPMs like 3,000 or 4,000, there were some signs of cytoplasmic failure but no noticeable changes to morphology

    Swimming against the tide: a study of a neighbourhood trying to rediscover its ‘reason for being’– the case of South Bank, Redcar and Cleveland.

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    Many of the programmes and initiatives to regenerate deprived neighbourhoods appear to have had limited lasting impact. It has been argued that one reason for this is that we still have little real understanding of the nature and scale of the problems some communities face (Bernt, 2009). This article attempts to add to our knowledge through close study of an area with multiple problems and a history of failed regeneration attempts. An in-depth case study, undertaken to explore the current situation and future prospects of South Bank, a small neighbourhood in the North East of England, highlights transferable knowledge which may be applied to other regeneration areas. The analysis considers the nature and consequences of industrial decline; entrenched deprivation; the stigmatization of communities; the value of community consultation and the potential impact of retail-led regeneration. We question whether negative stigma attached to places can be changed and we ask what the future may hold for deprived communities now that public sector funding has largely dried up, and we consider an alternative approach: the potential impacts of private sector retail-led regeneration in the absence of public sector funding

    Next Generation Differential GPS Architecture

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    The United States Coast Guard is engaged in a project to re-capitalize Reference Station (RS) and Integrity Monitor (IM) equipment used in the Nationwide Differential Global Position System (NDGPS). The Coast Guard in partnership with industry is developing a new software application to run on an open architecture platform as a replacement for legacy equipment. Present commercially available off-the-shelf Differential Global Positioning System (DGPS) RS and IM equipment lacks the open architecture required to support long term goals and future system improvements. The utility of the proposed new hardware architecture and software application is impressive - nearly every aspect of performance and supportability significantly exceeds that of the legacy architecture. The flexibility of the new hardware and software architectures complement each other to offer promising possibilities for the future. For example, the new hardware architecture uses Ethernet for internal and external site equipment communications. Each Local Area Network (LAN) will be equipped with a router and two 24 port switches. Various levels of password protection are provided to manage security both locally and remotely. While the new software application directly supports the legacy RS-232/422 interfaces to devices such as GPS receivers, a system design goal includes the ability to directly address each device from NCS. With the use of TCP/IP to RS-232/422 port server devices, the system can meet these forward reaching goals while supporting legacy equipment. New system capabilities include remote software management, remote hardware configuration management, and flexible options for management of licenses. The new configurable RS and IM architecture is a PCbased emulation of legacy reference station and integrity monitor equipment. It supports fluid growth and exploitation of new signals, formats, and technology as they become available, while remaining backward compatible with legacy architecture and user equipment. Examples of new capabilities include enhanced data management & anomaly analysis, universal On Change Reference Station Integrity Monitor (RSIM) message scheduling, improved satellite clock handling, additional observation interval modes, and Range Rate Correction monitoring in the IM. Engineering initiatives under development such as implementation of pre-broadcast integrity are also presented. This paper details challenges and goals that drove software and hardware design approaches destined to become the backbone of the Next Generation Differential GPS Architecture. Functional differences between legacy and next generation operation are explored. The new DGPS system architecture will allow the USCG radiobeacon system to continue to deliver and improve navigation and positioning services to our nation and its territories. Reprinted with permission from The Institute of Navigation (http://ion.org/) and The Proceedings of the 18th International Technical Meeting of the Satellite Division of The Institute of Navigation, (pp. 816-826). Fairfax, VA: The Institute of Navigation

    Neutrino flavour relaxation or neutrino oscillations?

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    We propose the new mechanism of neutrino flavour relaxation to explain the experimentally observed changes of initial neutrino flavour fluxes. The test of neutrino relaxation hypothesis is presented, using the data of modern reactor, solar and accelerator experiments. The final choice between the standard neutrino oscillations and the proposed neutrino flavour relaxation model can be done in future experiments

    Unknowns after the SNO Charged-Current Measurement

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    We perform a model-independent analysis of solar neutrino flux rates including the recent charged-current measurement at the Sudbury Neutrino Observatory (SNO). We derive a universal sum rule involving SNO and SuperKamiokande rates, and show that the SNO neutral-current measurement can not fix the fraction of solar νe\nu_e oscillating to sterile neutrinos. The large uncertainty in the SSM 8^8B flux impedes a determination of the sterile neutrino fraction.Comment: Version to appear in PRL; includes analysis with anticipated SNO NC measuremen

    Molecular dynamics simulations of lead clusters

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    Molecular dynamics simulations of nanometer-sized lead clusters have been performed using the Lim, Ong and Ercolessi glue potential (Surf. Sci. {\bf 269/270}, 1109 (1992)). The binding energies of clusters forming crystalline (fcc), decahedron and icosahedron structures are compared, showing that fcc cuboctahedra are the most energetically favoured of these polyhedral model structures. However, simulations of the freezing of liquid droplets produced a characteristic form of ``shaved'' icosahedron, in which atoms are absent at the edges and apexes of the polyhedron. This arrangement is energetically favoured for 600-4000 atom clusters. Larger clusters favour crystalline structures. Indeed, simulated freezing of a 6525-atom liquid droplet produced an imperfect fcc Wulff particle, containing a number of parallel stacking faults. The effects of temperature on the preferred structure of crystalline clusters below the melting point have been considered. The implications of these results for the interpretation of experimental data is discussed.Comment: 11 pages, 18 figues, new section added and one figure added, other minor changes for publicatio

    Surface-reconstructed Icosahedral Structures for Lead Clusters

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    We describe a new family of icosahedral structures for lead clusters. In general, structures in this family contain a Mackay icosahedral core with a reconstructed two-shell outer-layer. This family includes the anti-Mackay icosahedra, which have have a Mackay icosahedral core but with most of the surface atoms in hexagonal close-packed positions. Using a many-body glue potential for lead, we identify two icosahedral structures in this family which have the lowest energies of any known structure in the size range from 900 to 15000 lead atoms. We show that these structures are stabilized by a feature of the many-body glue part of the interatomic potential.Comment: 9 pages, 8 figure

    Kidney cell electrophoresis in space flight: Rationale, methods, results and flow cytometry applications

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    Cultures of human embryonic kidney cells consistently contain an electrophoretically separable subpopulation of cells that produce high levels of urokinase and have an electrophoretic mobility about 85 percent as high as that of the most mobile human embryonic kidney cells. This subpopulation is rich in large epithelioid cells that have relatively little internal structure. When resolution and throughput are adequate, free fluid electrophoresis can be used to isolate a broad band of low mobility cells which also produces high levels of plasminogen activators (PAs). In the course of performing this, it was discovered that all electrophoretic subpopulations of cultured human embryonic kidney cells produce some PAs and that separate subpopulations produce high quantities of different types of PA's. This information and the development of sensitive assays for this project have provided new insights into cell secretion mechanisms related to fibrinolysis. These advances would probably not have been made without the NASA program to explore fundamental questions of free fluid electrophoresis in space

    Entropic effects on the Size Evolution of Cluster Structure

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    We show that the vibrational entropy can play a crucial role in determining the equilibrium structure of clusters by constructing structural phase diagrams showing how the structure depends upon both size and temperature. These phase diagrams are obtained for example rare gas and metal clusters.Comment: 5 pages, 3 figure
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