Distribution of Di- to Hexa- microsatellite motifs in <i>Anisogramma</i><i>anomala</i> by number of repeats.

<p>Distribution of Di- to Hexa- microsatellite motifs in <i>Anisogramma</i><i>anomala</i> by number of repeats.</p

Experimental screening of microsatellite loci in <i>Anisogramma</i><i>anomala</i>.

<p>For polymorphic loci, the expected size difference between alleles is multiples of the unit size (e.g. 2x, 3x, and 4x for di-, tri- and tetra- nucleotide motifs, respectively), assuming the motifs are perfect and there are no size differences in the flanking regions.</p

Frequency distribution of mono-, di-, and tri- microsatellite motifs in <i>Anisogramma</i><i>anomala</i> by motif sequences.

<p>Frequency distribution of mono-, di-, and tri- microsatellite motifs in <i>Anisogramma</i><i>anomala</i> by motif sequences.</p

Flow chart describing the bioinformatics pipeline used to construct a genome-wide microsatellite marker database for <i>Anisogramma</i><i>anomala</i>.

<p>See text for detail. </p

Frequency distribution of microsatellite loci in <i>Anisogramma anomala</i> by motif length.

<p>Frequency distribution of microsatellite loci in <i>Anisogramma anomala</i> by motif length.</p

Consensus UPGMA tree of 30 <i>Anisogramma</i><i>anomala</i> isolates characterized by 10 microsatellite loci.

<p>Isolates are named by the city and state from where they were collected, and followed by a number if multiple isolates were obtained from the same location. Isolates were grouped into two clades in accordance with their geographic origins. UPGMA trees were generated by PowerMarker version 3.25 [36] using frequency-based genetic distance of shared alleles [39]. Consensus tree was computed from 100 bootstrap replicates using “consense” program in software package PHYLIP version 3.695 (<a href="http://evolution.genetics.washington.edu/phylip.html" target="_blank">http://evolution.genetics.washington.edu/phylip.html</a>). Numbers by the branches are bootstrap support values in 100 replicates. Branches with less than 50% bootstrap support were collapsed. </p