3 research outputs found
Additional file 4: of Dendritic cells change IL-27 production pattern during childhood
Figure S4. Age related frequencies of IL-27-positive mDCs (top row) and pDCs (lower row)
Additional file 3: of Dendritic cells change IL-27 production pattern during childhood
Figure S3. Age-related cell frequency
Enhanced in Vivo Targeting of Murine Nonparenchymal Liver Cells with Monophosphoryl Lipid A Functionalized Microcapsules
A broad spectrum of infectious liver
diseases emphasizes the need
of microparticles for targeted delivery of immunomodulatory substances
to the liver. Microcapsules (MCs) are particularly attractive for
innovative drug and vaccine formulations, enabling the combination
of antigen, drugs, and adjuvants. The present study aimed to develop
microcapsules characterized by an enhanced liver deposition and accelerated
uptake by nonparenchymal liver cells (NPCs). Initially, two formulations
of biodegradable microcapsules were synthesized from either hydroxyethyl
starch (HES) or mannose. Notably, HES-MCs accumulated primarily in
the liver, while mannose particles displayed a lung preference. Functionalization
of HES-MCs with anti-CD40, anti-DEC205, and/or monophosphoryl lipid
A (MPLA) enhanced uptake of MCs by nonparenchymal liver cells in vitro.
In contrast, only MPLA-coated HES-MCs promoted significantly the in
vivo uptake by NPCs. Finally, HES-MCs equipped with MPLA, anti-CD40,
and anti-DEC205 induced the secretion of TNF-α, IL-6 by Kupffer
cells (KCs), and IFN-γ and IL-12p70 by liver dendritic cells
(DCs). The enhanced uptake and activation of KCs by MPLA-HES-MCs is
a promising approach to prevent or treat infection, since KCs are
exploited as an entry gate in various infectious diseases, such as
malaria. In parallel, loading and activating liver DCs, usually prone
to tolerance, bears the potential to induce antigen specific, intrahepatic
immune responses necessary to prevent and treat infections affecting
the liver