21 research outputs found
Genetic and antigenic analysis of Babesia bigemina isolates from five geographical regions of Brazil
Antigenic characterization of Brazilian isolates of Anaplasma marginale
Antigenic characterization of Anaplasma marginale isolates, by
identifying conserved and variable epitopes of major surface proteins
(MSP), is an important tool for vaccine development against this
rickettsia. The B cell epitopes of A. marginale isolates from three
microregions of the State of Pernambuco and one from the State of Mato
Grosso do Sul, Brazil, were characterized by indirect fluorescent
antibody technique (IFAT) and Western blot (WB) with 15 monoclonal
antibodies (MAbs). The epitope recognized by MAb ANA22B1 (MSP-1a) was
conserved by IFAT and WB (73-81 kDa). MSP-2 epitopes recognized by MAbs
ANAO58A2 and ANAO70A2 were conserved by IFAT, while ANAO50A2 and
ANA66A2 epitopes were polymorphic; in the WB, the MAbs ANAO50A2 and
ANAO70A2 identified bands of 45 kDa only in the Pernambuco-Mata
isolate. None of the isolates reacted with MAb ANAR75C2 (MSP-3). The
MSP-4 epitope recognized by MAb ANAR76A1 was conserved by IFAT, as well
as the MSP-5 epitope recognized by MAb ANAF16C1 by IFAT and WB (16
kDa). The MAbs ANAR17A6, ANAR83B3, ANAR94C1, ANAO24D5 and ANAR19A6
identified conserved epitopes by IFAT. MSP-1, MSP-2 and MSP-4, which
previously showed partial protection in experimental trials, are also
potential immunogens to be employed in Brazil, due to the B cell
epitope conservation
Serological survey of Babesia bovis, Babesia bigemina, and Anaplasma marginale antibodies in cattle from the semi-arid region of the state of Bahia, Brazil, by enzyme-linked immunosorbent assays
The objectives of this work were to determine the prevalence of Babesia
bovis , Babesia bigemina , and Anaplasma marginale
detecting antibodies in cattle raised in the semi-arid region
of the state of Bahia, Brazil, through indirect enzyme linked
immunosorbent assays (ELISA) and to compare the performances of
indirect enzyme-linked immunosorbent assays with crude
(I-ELISA-CrAnaAg) and recombinant major surface protein-5
(I-ELISA-MSP-5Ag), as antigens to detect antibodies against A.
marginale. An stable enzootic area was found in Senhor do Bonfim and
Euclides da Cunha for B. bovis that showed 86 and 95.5% of prevalence,
respectively, and for B. bigemina with 90.8 and 91.3%. On the other
hand, Uauá and Juazeiro, were characterized as enzootically
unstable areas, since prevalences were: B. bovis - 63.7 and 56.4% and
B. bigemina - 53 and 54.8%, respectively. The prevalence of A.
marginale in the four municipalities was above 97% with I-ELISA-CrAnaAg
and 94.8% with I-ELISA-MSP-5Ag which is an indication of stable
enzootic condition for the rickettsia. The I-ELISA-CrAnaAg and
I-ELISA-MSP-5Ag showed a highly significant association (r = 0.977),
which means that both ELISA tests are suitable for epidemiological
studies of A. marginal