ROC curve analysis.

<p>The ROC curve area for CD8⁺CD28^-CD57⁺ (AUC) was 0.79 and the cut-off >8.87% has a sensitivity of 75,61% (CI: 59.7% to 87.64%), specificity of 78.85% (CI: 65.30% to 88.94%) and a likelihood ratio of 3.57 (p<0.0001).</p

T cells phenotype in HIV infected subjects.

<p>CD4⁺ and CD8⁺ immune activation (A, B) and immunesenescence (C, D) in HIV-infected subjects according to the presence of bone and vascular diseases: presence of two comorbidties (bone and endovascular damage), only one comorbidity (bone or endovascular damage), no comorbidity. Values are expressed as a percentage. The horizontal bars represent the median. The upper and lower whisker indicate the third quartile +1.5 [Inter Quartile Range (IQR)] and first quartile -1.5(IQR).</p

c-IMT and BMD in HIV infected subjects.

<p><b>A. A</b> higher c-IMT in HIV positive patients with a low BMD than in patients with a normal BMD was found. Horizontal bars represent the median. The upper and lower whisker indicate the third quartile +1.5 [Inter Quartile Range (IQR)] and first quartile -1.5(IQR). <b>B. A</b> linear regression analysis showed a negative correlation between c-IMT and BMD.</p

OPG plasma levels in HIV positive subjects.

<p>OPG plasma levels (pmol/l) in HIV-infected subjects according to the presence of bone and endovascular damage: presence of two comorbidties (bone and endovascular damage), only one comorbidity (bone or endovascular damage), no comorbidity. The horizontal bars represent median. The upper and lower whisker indicate the third quartile +1.5 [Inter Quartile Range (IQR)] and first quartile -1.5(IQR).</p

Clinical Characteristics.

<p>Clinical Characteristics.</p

Multivariate Analysis.

<p>Multivariate Analysis.</p

IFN-γ concentrations in TB patients according to the results of QFT-G assay at the end of treatment.

<p><b>Group A:</b> TB patients who had a QFT-G reversion (positive to negative) at the end of therapy.</p><p><b>Group B:</b> TB patients who remained QFT-G positive at the end of therapy.</p

Cytofluorimetric analysis of phenotypic profile of T cells from two representative TB patients at the end of treatment.

<p>A representative sample of one TB patient with QFT-G reversion (positive to negative) at the end of therapy (panel A) and one TB patient with persistent QFT-G positive result (panel B) is shown. The percentage of CD4+ and CD8+ T cells that expressed CD45RO and lymphotropic chemokine receptor CCR7 was assessed in diluted whole blood after 6 days of in vitro stimulation with intact recombinant ESAT-6 and CFP-10 proteins. Effector memory (EM) cells (CD45RO+/CCR7−) are shown in the upper left quadrants of both panels; central/memory (CM) cells (CD45RO+/CCR7+) are showed in the upper right quadrants of both panels.</p

Baseline characteristics of the subjects included in the study.

<p><b>Abbreviations:</b></p><p>BCG: Bacillus Calmette and Guerin; TB: tuberculosis.</p

IFN-γ responses to mycobacterial antigens, PPD and PHA assessed by short or prolonged incubation-based assays.

<p><b>Abbreviations:</b></p><p>PPD: purified protein derivative; PHA: phytohemagglutinin; rESAT-6: recombinant ESAT-6; rCFP-10: recombinant CFP-10.</p><p><b>Group A:</b> TB patients who had a QFT-G reversion (positive to negative) at the end of therapy.</p><p><b>Group B:</b> TB patients who remained QFT-G positive at the end of therapy.</p