Origin and subculture history of <i>M</i>.<i>ulcerans</i> strains.

<p>Origin and subculture history of <i>M</i>.<i>ulcerans</i> strains.</p

Evolution of bacterial load over time after infection by <i>M</i>.<i>ulcerans</i>.

<p>ZN stainings of 5 micrometer thick slices of paraffin embedded footpad of a mouse infected <i>s</i>.<i>c</i>. with <i>M</i>. <i>ulcerans</i> NM20/02 4 weeks (upper), 10 weeks (middle) and 15 weeks (lower) after infection.</p

Comparison of macroscopic signs of infection in mice inoculated by various <i>M</i>. <i>ulcerans</i> strains.

<p>Suspensions of <i>M</i>. <i>ulcerans</i> from seven strains from Ghana and Cameroon were injected subcutaneous (<i>s</i>.<i>c</i>.) in the footpad of C57Bl/6 mice. Footpad swelling at week 4 after infection is shown.</p

Influence of mouse strain on the outcome of <i>M</i>.<i>ulcerans</i> infection.

<p>A solution of 6.6x10⁴ CFU of <i>M</i>. <i>ulcerans</i> was injected in the footpad of C57Bl/6 (A) and Balb/cJ mice (B). Footpad pictures are shown at week 8 after infection (upper panel). ZN staining of 5μm thick histology slices of paraffin embedded footpads show infiltration area (blue nuclei—middle panel) and bacterial load (pink rod-shape bacilli—lower panel) 12 week after infection in C57Bl/6 (A) and Balb/c (B) mice.</p

Infiltration of leukocytes within the footpad infected with <i>M</i>. <i>ulcerans</i>.

<p>Immunohistochemistry was performed on 5 micrometer paraffin embedded sections of footpad of mice infected with a suspension of <i>M</i>. <i>ulcerans</i> (NM20/02) in order to identify the nature of the leukocytic infiltrate present within and around the bacterial foci. B cells were stained with anti-CD45R antibody (A), T cells with anti-CD3 antibody (B), Macrophages with MOMA (C) and neutrophils with anti-Ly6G/Ly6C (D).</p

Lymph node cellularity of Balb/Jc vs C57Bl/6 mice.

<p>H&E staining was performed on 5 micrometer paraffin embedded sections of draining (right) and non-draining lymph nodes.</p

Macroscopic and microscopic signs of infection and inflammation in mice infected with <i>M</i>. <i>ulcerans</i>.

<p>The upper panel shows photograph of a C57Bl/6 mouse footpad 5 weeks after injection with <i>M</i>. <i>ulcerans</i> (NM20/02) (A) and the non-injected control footpad (B). Middle panel shows the corresponding 5micrometer thick paraffin embedded footpad slices stained with Ziehl-Neelsen (ZN) staining. Finally, the lower panel shows the same footpad slices stained with Hematoxylin Eosin (H&E). Arrows show the bacterial foci (ZN) and the inflamed tissue (H&E) of the lesion.</p

The mRNA of and is processed at its 5' end and is stabilized by both a polypurine sequence and translation initiation-0

Asmid pMV261, which carries the BCG promoter, and plasmid pMYS648, in which the 437–552 region has been substituted to the promoter region. The samples were run on a 5% denaturing polyacrylamide gel. Molecular weight markers (MWM) run in the same gel are indicated on the right. The about 300 bp transcript synthesized by pMV261 is indicated by an arrow.<p><b>Copyright information:</b></p><p>Taken from "The mRNA of and is processed at its 5' end and is stabilized by both a polypurine sequence and translation initiation"</p><p>http://www.biomedcentral.com/1471-2199/9/33</p><p>BMC Molecular Biology 2008;9():33-33.</p><p>Published online 4 Apr 2008</p><p>PMCID:PMC2358910.</p><p></p

The mRNA of and is processed at its 5' end and is stabilized by both a polypurine sequence and translation initiation-5

Asmid pMV261, which carries the BCG promoter, and plasmid pMYS648, in which the 437–552 region has been substituted to the promoter region. The samples were run on a 5% denaturing polyacrylamide gel. Molecular weight markers (MWM) run in the same gel are indicated on the right. The about 300 bp transcript synthesized by pMV261 is indicated by an arrow.<p><b>Copyright information:</b></p><p>Taken from "The mRNA of and is processed at its 5' end and is stabilized by both a polypurine sequence and translation initiation"</p><p>http://www.biomedcentral.com/1471-2199/9/33</p><p>BMC Molecular Biology 2008;9():33-33.</p><p>Published online 4 Apr 2008</p><p>PMCID:PMC2358910.</p><p></p

The mRNA of and is processed at its 5' end and is stabilized by both a polypurine sequence and translation initiation-1

to the promoter. The lines below the map correspond to different regions amplified by a pair of oligonucleotides. Fragment A: -689/-492; B: -81/91 (promoter); C: 374/557; D: 1155/1344. Below the lines, the values of the ratio of each fragment relative to the DNA.<p><b>Copyright information:</b></p><p>Taken from "The mRNA of and is processed at its 5' end and is stabilized by both a polypurine sequence and translation initiation"</p><p>http://www.biomedcentral.com/1471-2199/9/33</p><p>BMC Molecular Biology 2008;9():33-33.</p><p>Published online 4 Apr 2008</p><p>PMCID:PMC2358910.</p><p></p