Results of binary logistic regression analyses, dependent variable = hearing improvement > 10 dB, independent variables = GPIIIa receptor density and fibrinogen, n = 55^#.

<p>Measure of effect modification on additive scale: relative excess risk due to interaction, RERI (95% CI)  =  0.61 (−1.11 – 2.34)</p><p>Measure of effect modification on multiplicative scale: ratio of ORs (95% CI) = 1.16 (0.61– 2.20), p = 0.650</p><p>ORs are adjusted for smoking and vertigo, # = 4 datasets missing due to missing covariates.</p

Treatment outcome.

1<p>Analysis of variance.</p>2<p>Transcranial direct current stimulation.</p>3<p>Tinnitus Questionnaire, total score.</p>4<p>Tinnitus Questionnaire, subscale emotional + cognitive distress.</p>5<p>Tinnitus Handicap Questionnaire, total score.</p>6<p>Tinnitus Handicap Inventory, total score.</p><p>*Statistically significant.</p

Comparison of study participants with and without data on glycoprotein receptor density, n = 127.

<p>&  =  data missing on 3 patients; &&  =  data missing on 6 patients; CC  =  wild type, TC  =  heterozygous; TT  =  homozygous; §  =  Mantel-Haenszel Chi-square test.</p

Transcranial direct current stimulation (tDCS).

<p>Time course of direct current strength in the different tDCS conditions.</p

Test calibration cytogram with cursor settings in gated fluorescence histogram (a) and platelet rich plasma cytogram with GpIa immune-labelling cursor settings (b).

<p>A single color flow cytometric analysis of the platelet glycoproteins GpIa, GpIb and GpIIIa was used (Biocytex, Marseille, France). The number of antigenic sites is determined by converting the fluorescence intensity into the corresponding number of sites per platelet based on calibrated bead standards (a). In all experiments, more than 90% of the platelets of the PRP were gated for the experiment, as shown here for GpIa (b).</p

Changes in Tinnitus Handicap Questionnaire (THQ) values during and after treatment.

<p>Changes in THQ total scores relative to the baseline scores as functions of time (after tDCS¹ + TMNMT² vs. after TMNMT only vs. obs3 vs. obs31) and tDCS condition (anodal group vs. cathodal group vs. sham group). Bars represent means, error bars denote standard errors of the mean. Negative values reflect improvement. Dashed black lines indicate significant post-hoc tests. *p<.05, **p<.01; ¹Transcranial direct current stimulation; ²Tailor-made notched music training.</p

Results of binary logistic regression analyses, dependent variable = hearing improvement >10 dB, independent variables = GPIa receptor density and fibrinogen, n = 49^#.

<p>Measure of effect modification on additive scale: relative excess risk due to interaction, RERI (95% CI)  =  −0.90 (−1.97 – 0.17)</p><p>Measure of effect modification on multiplicative scale: ratio of ORs (95% CI) = 0.45 (0.21 – 0.94), p = <b>0.034</b></p><p>ORs are adjusted for smoking and vertigo, #  =  4 datasets missing due to missing covariates.</p

Music spectra.

<p>Schematic frequency spectra of original music (solid line) and flattened notched music (dashed dotted line).</p

Study design.

<p>For each participant, the study took 45 days (4 days of pre-treatment waiting, 10 days of treatment, and 31 days of post-treatment observation). During the initial 5 days of treatment, transcranial direct current stimulation (tDCS) and the tailor-made notched music training (TMNMT) were applied simultaneously; during the remaining 5 days of treatment, only TMNMT was applied. Throughout the study, perceived tinnitus-related distress data were sampled repeatedly.</p

Number of GpIa (a), GpIb (b) and GpIIIa (c) receptor molecules per platelet in patients (left) and controls (right).

<p>The values are shown as boxplots, separated for T allele carriers (phenotype TT or CT) and CC allele carriers. The boundary of the box closest to zero indicates the 25^th percentile, the line within the box marks the median, the square indicates the mean, and the boundary of the box farthest from zero indicates the 75^th percentile. Whiskers above and below the box indicate the 90^th and 10^th percentiles. The points represent the 1^st and 99^th percentiles. P indicates the level of significance according to the Mann-Whitney-U-test. *, P<0.05; ***, P<0.001.</p