Allergenicity of egg white and yolk from different chicken breeds.

<p>Specific IgE levels to native and heat-treated egg white (A and B) and yolk (C and D) from Araucana (black circles), Maran (white circles), and conventional laying hen hybrid breeds (grey circles) were determined by ELISA. Circles represent values obtained from individual patients' sensitized to egg white (n = 25) and yolk (n = 12). Mean values are shown as solid line and whiskers indicate the standard error of the mean. Statistical analysis was performed using the Wilcoxon signed rank test. **<0.01; *<0.02.</p

Demographics and immunological characterization of hen's egg allergic patients.

<p>SPT were performed with commercially available egg white extracts and are presented as wheel area (mm²). Specific IgE antibodies were measured by ELISA with coated native egg white (W), denatured egg white (W^d), native yolk (Y), and denatured yolk (Y^d) extracts; results are expressed as specific IgE concentrations in µg/ml. IgE reactivity to <i>Gallus domesticus</i> allergens were determined by microarray using Immuno solid-phase allergen chips (ISAC) and given in as kU_A/l. DBPCFC, double blind placebo controlled food challenge; AD, atopic dermatitis; AE, angioedema; AN, anaphylaxis; AS, asthma; CO, conjunctivitis; GI, gastrointestinal symptoms (including vomiting, diarrhea, and pain); n.p., not performed; OAS, oral allergy syndrome; RH, rhinitis; UR, generalized urticaria.</p

Identification of allergenic egg components.

<p>IgE-reactive egg white (A) and yolk (B) extract components from Araucana (A), Maran (M), and conventional laying hen hybrid (H) breeds were detected by IgE immunoblotting (left charts). IgE-reactive bands (A1–5 and Y1–5) were excised from polyacrylamide gels (right charts), and subjected to mass spectrometry analysis. Corresponding proteins, their molecular weights (MW), and SwissProt/TrEMBL accession numbers are given in the tables.</p

Characteristics of ancient and modern hybrid chicken breeds.

<p>*Weights were determined and arithmetic means and standard deviations were calculated from 10 individual eggs.</p

Pomegranate Cultivars: Identification of the New IgE-Binding Protein Pommaclein and Analysis of Antioxidant Variability

The consumption of pomegranate is increasing as it is considered a health-promoting food. Nevertheless, it can trigger allergic reactions, sometimes severe. The LTP Pun g 1 is the only pomegranate allergen so far reported. Based on preliminary clinical observations, the main aim of this study was the investigation of still unknown allergens contained in this fruit. Pommaclein, a homologue of peamaclein, the peach allergen Pru p 7, was isolated, identified by protein sequencing, and characterized as an IgE-binding protein by different test systems. RP-HPLC protein profiles revealed significant variations of LTP and pommaclein content in the red pulp of selected cultivars and accessions. Conversely, the mesocarp appeared free of proteins and much richer in antioxidants. In conclusion, a new allergen has been identified, and it could contribute to improving allergy diagnosis. The study highlights that pomegranate mesocarp could represent a rich and safe source of nutraceuticals also for allergic subjects

Bivariate analysis of reciprocal relationships of IgE to profilin, PR-10, and tropomyosin panallergens.

<p>The number of IgE reactive subjects and the Pearson coefficient are shown for paired allergens within the three panallergen groups. Pearson r values are reported below. Data were statistically significant in all cases (p<0.001). Panel A: Profilins; Panel B: PR-10; Panel C: Tropomyosins.</p

List, characteristics, and Allergome reference code for the three panallergen groups, ranked by prevalence of IgE reactivity among and within groups.

*<p>Natural purified molecules, all others are <i>E.coli</i>-produced recombinants. Biochemical, immunological and clinical details on each allergen are available at <a href="http://www.allergome.org" target="_blank">www.allergome.org</a>.</p><p>°Generated using AllergomeAligner (<a href="http://www.allergome.org" target="_blank">www.allergome.org</a>) assuming the first ranked IgE reactive allergen as reference sequence ( = 100%).</p>#<p>Complete Pen i 1 sequence is not available. From peptide fragment matching and IgE reactivity it is assumed to be close to Pen m 1.</p

Age group distribution of the ISAC IgE panallergens-reactive population; n = 3,113.

<p>Black bars: Total 23,077 original allergic population; White Bars: Total 3,113 panallergens allergic population; Dark grey bars: PR-10; Hatched bars: Profilin; Light grey bars: Tropomyosin.</p